Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,138 G→A 5.4% pseudogene (747/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,1380GA 5.4% 288.1 / 3.4 92pseudogene (747/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base G (44/42);  new base A (2/3);  total (46/46)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATC‑‑‑GACGGTGACAAGGCCA  >  NC_000913/1465991‑1466286
                                                                                                                                                   |                                                                                                                                                       
ggTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGa                                                                                                                                                        <  8:241915/149‑1 (MQ=255)
 gTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGa                                                                                                                                                        >  5:115084/1‑148 (MQ=255)
 gTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGa                                                                                                                                                        >  5:52699/1‑148 (MQ=255)
  tGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGaaa                                                                                                                                                      <  3:363881/149‑1 (MQ=255)
    aGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAg                                                                                                                                                     <  3:335391/148‑1 (MQ=255)
     gcgcAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTg                                                                                                                                                   <  1:229399/149‑1 (MQ=255)
           ctGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAg                                                                                                                                             <  4:214810/148‑1 (MQ=255)
           aTGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAg                                                                                                                                             <  4:388400/149‑1 (MQ=255)
           aTGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAg                                                                                                                                             <  5:394501/149‑1 (MQ=255)
           aTGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAg                                                                                                                                             >  6:263656/1‑149 (MQ=255)
                 cAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGc                                                                                                                                       >  7:337672/1‑149 (MQ=255)
                  aaCGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGc                                                                                                                                       >  8:416840/1‑148 (MQ=255)
                    cGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGAt                                                                                                                                    >  4:244094/1‑149 (MQ=255)
                    cGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGAt                                                                                                                                    <  8:118225/149‑1 (MQ=255)
                       aGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACAGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTg                                                                                                                                 >  2:12404/1‑149 (MQ=255)
                         tGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGa                                                                                                                               <  2:417976/149‑1 (MQ=255)
                         tGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGa                                                                                                                               >  1:365537/1‑149 (MQ=255)
                           gCAATATAATCACAATGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATg                                                                                                                             >  3:401542/1‑149 (MQ=255)
                           gCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATg                                                                                                                             >  3:68119/1‑149 (MQ=255)
                            cAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCAACGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGt                                                                                                                            >  6:33233/1‑149 (MQ=255)
                               tataATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGCCGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAg                                                                                                                         >  7:344906/1‑149 (MQ=255)
                               tataATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAg                                                                                                                         >  1:307035/1‑149 (MQ=255)
                                ataATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgc                                                                                                                        <  2:212089/149‑1 (MQ=255)
                                 taATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcg                                                                                                                       >  8:20621/1‑149 (MQ=255)
                                 taATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcg                                                                                                                       >  8:182731/1‑149 (MQ=255)
                                    tCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcg                                                                                                                    >  2:283016/1‑149 (MQ=255)
                                      acaACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcggc                                                                                                                  >  7:59085/1‑149 (MQ=255)
                                      acaACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcggc                                                                                                                  <  8:83445/149‑1 (MQ=255)
                                                 cTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAt                                                                                                       <  8:147665/149‑1 (MQ=255)
                                                    gTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAATGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTAtt                                                                                                                                                <  8:267444/105‑1 (MQ=255)
                                                    gTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAtt                                                                                                      <  5:263656/147‑1 (MQ=255)
                                                    gTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTAtt                                                                                                                                                >  7:267444/1‑105 (MQ=255)
                                                     ttattaCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCTCCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGa                                                                                                    <  4:394305/148‑1 (MQ=255)
                                                     ttattaCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGa                                                                                                    <  7:182731/148‑1 (MQ=255)
                                                       attaCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGata                                                                                                  <  7:375909/148‑1 (MQ=255)
                                                         taCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCa                                                                                               >  7:420013/1‑149 (MQ=255)
                                                          aCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAc                                                                                              >  3:330268/1‑149 (MQ=255)
                                                          aCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAc                                                                                              >  5:262386/1‑149 (MQ=255)
                                                            gggTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCg                                                                                            >  4:34518/1‑149 (MQ=255)
                                                              gTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAATGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCa                                                                                                                                              <  8:314340/97‑1 (MQ=255)
                                                              gTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGt                                                                                          <  7:107408/149‑1 (MQ=255)
                                                              gTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGt                                                                                          >  8:191520/1‑149 (MQ=255)
                                                              gTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCa                                                                                                                                              >  7:314340/1‑97 (MQ=255)
                                                                   aaTGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTg                                                                                                                                 <  6:109047/105‑1 (MQ=255)
                                                                    aTGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAgc                                                                                    >  4:74957/1‑149 (MQ=255)
                                                                         acaGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGAATCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGAc                                                                               >  3:276383/1‑149 (MQ=255)
                                                                           aGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACgg                                                                             >  5:289079/1‑149 (MQ=255)
                                                                            gTAAACAACAATGGCAAAACCACCGTTGATGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGt                                                                            >  4:297569/1‑149 (MQ=255)
                                                                               cacaacaATGGCAAACCCACCGTTGACGGTAAAGATTCCCCCGGTACGGACATCAACGGTCATAACGGGAAAGGGATTCAGGCCGGCGATCTGGATGGCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTgg                                                                          <  2:430282/147‑1 (MQ=14)
                                                                               aacaacAATGGCAAAACCACCGTTGACGGTAATGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAg                                                                                                                                                     <  8:371860/73‑1 (MQ=255)
                                                                               aacaacAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGTAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCa                                                                                               <  8:23684/127‑1 (MQ=25)
                                                                               aacaacAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCa                                                                                               >  7:23684/1‑127 (MQ=255)
                                                                               aacaacAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGa                                                                         >  3:147530/1‑149 (MQ=255)
                                                                               aacaacAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGa                                                                         >  3:327953/1‑149 (MQ=255)
                                                                                acaacaATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGAt                                                                        <  8:286424/149‑1 (MQ=255)
                                                                                   acaaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAAc                                                                     <  4:375407/149‑1 (MQ=255)
                                                                                      aTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAg                                                                  <  2:307035/149‑1 (MQ=255)
                                                                                      aTGGCAAAACAACCGTTGACGTTAAATATTCCACCGGTACGGAAATCAACGTTAATAACCGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTTATATCACCGGTGACAGAGCGACGGTGGATAACAAg                                                                  >  2:349669/1‑149 (MQ=14)
                                                                                         gCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGTGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAggg                                                                >  2:300068/1‑148 (MQ=255)
                                                                                          cAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCa                                                              <  6:52699/149‑1 (MQ=255)
                                                                                          cAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGGCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCa                                                              <  6:115084/149‑1 (MQ=255)
                                                                                           aaaaCCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCAc                                                             >  6:240098/1‑149 (MQ=255)
                                                                                           aaaaCCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCAc                                                             <  8:420013/149‑1 (MQ=255)
                                                                                            aaaCCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCAc                                                             >  6:191125/1‑148 (MQ=255)
                                                                                            aaaCCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCAc                                                             >  7:200080/1‑148 (MQ=255)
                                                                                                caccGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGTGGCGGTCACGGTATTGa                                                                                                    <  6:362458/105‑1 (MQ=255)
                                                                                                caccGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGTGGCGGTCACGGTATTGa                                                                                                    >  5:362458/1‑105 (MQ=255)
                                                                                                 accGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGAc                                                       <  5:240098/149‑1 (MQ=255)
                                                                                                           gTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACTGTATTGATATCACCGGTGACAGCGAGCCGGTGGATTACAAGGGCACCATGACCGTCACCGAt                                             >  6:41366/1‑149 (MQ=255)
                                                                                                              aaGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATcc                                           <  4:68119/148‑1 (MQ=40)
                                                                                                              aaGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCg                                          <  4:330268/149‑1 (MQ=40)
                                                                                                                gATTCCTCCGGTTCGGTTATCTACGGTAATAACGGGTAAGTGATTCAGGAGGGCGATCTGGATGTGAGGGGGGGGGGTCACGGTATTGTTTTCTGCGGTGACAGCGCGTGGGTGGATAAGAAGGGCACCATGACCGTCACCGATCCGGa                                        <  2:365537/149‑1 (MQ=14)
                                                                                                                gATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCg                                          >  3:153156/1‑147 (MQ=39)
                                                                                                                gATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGa                                        <  3:297569/149‑1 (MQ=39)
                                                                                                                 aTTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCAc                                                 <  6:228038/139‑1 (MQ=40)
                                                                                                                 aTTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCAc                                                 >  5:228038/1‑139 (MQ=40)
                                                                                                                     cACCGGTACGGAAATCAACGTTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTGAGCGGCGGCGGGCACGGGTTTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCTGAGTCCa                                   <  1:349669/149‑1 (MQ=14)
                                                                                                                     cACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATTTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACTGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTcc                                    <  5:33233/148‑1 (MQ=255)
                                                                                                                     cACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGATGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCa                                   <  7:416840/149‑1 (MQ=255)
                                                                                                                     cACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCa                                   >  2:176114/1‑149 (MQ=39)
                                                                                                                        cggtacggtaATCAACGGTAATAACGGGAAAGTGATTGAGGACGGCTATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATgg                                <  4:327953/149‑1 (MQ=255)
                                                                                                                        cGGTACGGAAATCAACGTTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATgg                                <  6:289079/149‑1 (MQ=255)
                                                                                                                        cGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATgg                                <  3:34518/149‑1 (MQ=40)
                                                                                                                                    cAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGt                                      <  7:325698/131‑1 (MQ=40)
                                                                                                                                    cAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGt                                      >  8:325698/1‑131 (MQ=40)
                                                                                                                                     aaCGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCg                                                                                            <  1:170290/76‑1 (MQ=39)
                                                                                                                                     aaCGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCg                                                                                            >  2:170290/1‑76 (MQ=39)
                                                                                                                                     aaCGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATC‑‑‑Ga                <  8:59085/149‑1 (MQ=255)
                                                                                                                                     aaCGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATC‑‑‑Ga                >  3:116249/1‑149 (MQ=255)
                                                                                                                                     aaCGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGAATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCCTGCCCGTCACCGATCCGGAGTCCATGGGTATCCAGATC‑‑‑gc                >  2:382883/1‑148 (MQ=255)
                                                                                                                                             taaCGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCAAGATC‑‑‑GACGGTGAc         >  1:299195/1‑148 (MQ=255)
                                                                                                                                               aCGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATC‑‑‑GACGGTGACaa       <  5:191125/148‑1 (MQ=255)
                                                                                                                                                 gggAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATC‑‑‑GACGGTGACAAGGc    >  3:160433/1‑149 (MQ=255)
                                                                                                                                                 gggAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCAATCCGGAGTCCATGGGTATCCAGATC‑‑‑GACGGTGACAAGGc    >  8:293095/1‑149 (MQ=255)
                                                                                                                                                  ggAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGGTGACGGTGACAAg      <  8:200080/149‑1 (MQ=255)
                                                                                                                                                   gAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATC‑‑‑GACGGTGACAAGGCCa  >  7:122160/1‑149 (MQ=255)
                                                                                                                                                   gAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACAGTATTGATATCACCGGTGACAGCGCGACGGTGTATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATC‑‑‑GACGGTGACAAGGCCa  <  5:202763/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                       
GGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATC‑‑‑GACGGTGACAAGGCCA  >  NC_000913/1465991‑1466286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: