BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,806,979	G→A	83.0%	D204N (GAT→AAT)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,979	0	G	A	83.0%	245.0 / 41.4	107	D204N (GAT→AAT)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand): ref base G (11/7); new base A (45/43); total (57/50)
Fisher's exact test for biased strand distribution p-value = 6.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

GCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCG  >  NC_000913/1806835‑1807123

gcgcAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACC‑AAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTc                                                                                                                                             >  5:101068/1‑149 (MQ=255)

  acAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTcc                                                                                                                                            >  8:25899/2‑149 (MQ=255)

   cAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCg                                                                                                                                           <  1:24079/149‑1 (MQ=255)

    aaaaaCAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCg                                                                                                                                           >  7:182278/1‑148 (MQ=255)

      aaaCAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGc                                                                                                                                          <  5:202644/147‑1 (MQ=255)

      aaaCAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGc                                                                                                                                          >  6:202644/1‑147 (MQ=255)

       accAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCaaa                                                                                                                                       <  7:341199/147‑1 (MQ=255)

       aaCAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCa                                                                                                                                         >  4:46713/1‑147 (MQ=255)

              gATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGCACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACCATGTCCGCAAAGCcgcg                                                                                                                                 >  4:208881/1‑148 (MQ=255)

                tCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAg                                                                                                                              <  1:132381/149‑1 (MQ=255)

                   gCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGa                                                                                                                            >  3:259946/1‑148 (MQ=255)

                    cTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGaaa                                                                                                                          >  1:170752/1‑149 (MQ=255)

                     tGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGACGGACAATGTCCGCAAAGCCGCGCAGGAAAt                                                                                                                         >  3:167488/1‑149 (MQ=255)

                         tcgtcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGtt                                                                                                                     <  1:324475/149‑1 (MQ=255)

                            tcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTa                                                                                                                    <  1:400356/147‑1 (MQ=255)

                            tcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTa                                                                                                                    >  2:400356/1‑147 (MQ=255)

                            tcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTaa                                                                                                                   >  8:367390/1‑148 (MQ=255)

                            tcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTaa                                                                                                                   >  2:390223/1‑148 (MQ=255)

                                   ttCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGc                                                                                                            >  3:394080/1‑148 (MQ=255)

                                   ttCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGc                                                                                                            >  8:310518/1‑148 (MQ=255)

                                   ttCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCA‑‑CAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAg                                                                                                         >  4:272591/1‑149 (MQ=255)

                                      tGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAgg                                                                                                        >  3:268211/1‑149 (MQ=255)

                                        gCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAg                                                                                                         <  5:382510/146‑1 (MQ=255)

                                        gCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAg                                                                                                         <  4:371759/146‑1 (MQ=255)

                                           aaGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCaaa                                                                                                   <  8:182278/149‑1 (MQ=255)

                                                    gTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTc                                                                                          <  7:25899/149‑1 (MQ=255)

                                                         gcaCTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTc                                                                                     <  8:217409/149‑1 (MQ=255)

                                                           aCTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAg                                                                                                         <  7:273946/127‑1 (MQ=255)

                                                           aCTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAg                                                                                                         >  8:273946/1‑127 (MQ=255)

                                                            cTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCt                                                                                  <  5:121341/149‑1 (MQ=255)

                                                               gCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCACAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTGTCCCTggg                                                                               >  3:254391/1‑149 (MQ=255)

                                                               gCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAATCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGTGTTGTCGTTTCCCTggt                                                                               >  6:18653/1‑148 (MQ=255)

                                                               gCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTggg                                                                               >  8:43000/1‑149 (MQ=255)

                                                               gCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTggg                                                                               >  6:87414/1‑149 (MQ=255)

                                                               gCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTggg                                                                               >  8:201820/1‑149 (MQ=255)

                                                               gCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTgg                                                                                <  8:236149/148‑1 (MQ=255)

                                                                   ttGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATAGCGAACTCACCCAGCCGGACAATGTCCGAAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTAca                                                                           >  5:236465/1‑149 (MQ=255)

                                                                     ggTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCcac                                                                          <  7:201820/148‑1 (MQ=255)

                                                                      gTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCTGAAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACaa                                                                        >  5:387736/1‑149 (MQ=255)

                                                                       tAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAg                                                                       <  1:418972/149‑1 (MQ=255)

                                                                       tAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAg                                                                       <  6:315999/149‑1 (MQ=255)

                                                                         aCATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAg                                                                       >  3:243213/1‑147 (MQ=255)

                                                                         aCATCGAGTTGGTTAAGCCTAACC‑AAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGa                                                                     <  6:101068/148‑1 (MQ=255)

                                                                           aTCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAgc                                                                   >  3:253840/1‑149 (MQ=255)

                                                                             ctttttGGTTAGGCTTAACCAAAAAGAACTCAGTGCGCTGGTGAAACCCGAACACCCCCCGCCGGACGAAGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGGGTCCCGGGGTCCACAAGGAgcgc                                                                 >  2:48844/5‑149 (MQ=255)

                                                                              tttttGGTTAGGCTTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCt                                                                >  2:112599/4‑149 (MQ=255)

                                                                              gAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCt                                                                                  <  1:194985/131‑1 (MQ=255)

                                                                              gAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCt                                                                                  >  2:194985/1‑131 (MQ=255)

                                                                                gTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTgg                                                              <  4:130791/149‑1 (MQ=255)

                                                                                   ggTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgt                                                            <  7:367390/148‑1 (MQ=255)

                                                                                    gTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgtgt                                                          <  4:253840/149‑1 (MQ=255)

                                                                                    gTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgtgt                                                          >  2:440450/1‑149 (MQ=255)

                                                                                    gTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgtgt                                                          >  1:384463/1‑149 (MQ=255)

                                                                                     ttAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTGCCTGGGTCCACAAGGAGCGCTGCGTGtt                                                         >  2:279365/1‑149 (MQ=255)

                                                                                     ttAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGtt                                                         >  8:69455/1‑149 (MQ=255)

                                                                                         gCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCCCCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGa                                                       >  2:311475/1‑147 (MQ=255)

                                                                                           cTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGt                                                   <  4:268211/149‑1 (MQ=255)

                                                                                            tAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGt                                                   >  3:335728/1‑148 (MQ=255)

                                                                                            tAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGt                                                   >  4:343502/1‑148 (MQ=255)

                                                                                            tAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGt                                                   >  1:190199/1‑148 (MQ=255)

                                                                                            tAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGt                                                   >  2:257966/1‑148 (MQ=255)

                                                                                              aCCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGGTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTg                                                  <  1:390223/147‑1 (MQ=255)

                                                                                                cAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaaa                                               >  6:158078/1‑148 (MQ=255)

                                                                                                  aaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGaaa                                                                                                                          <  2:284662/71‑1 (MQ=255)

                                                                                                  aaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACt                                            <  2:384463/149‑1 (MQ=255)

                                                                                                    aaGAACTCAGTGCGCTGGTGAATCGGGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTg                                           >  1:196852/1‑148 (MQ=255)

                                                                                                    aaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGt                                          <  4:259946/149‑1 (MQ=255)

                                                                                                        aCTCAGTTCGCTGGTGAATCGCGAACTCACCCAGCCTGACAATGTCCGCTAAGCCGCGCAGGAAATCGTTAATAGCTGCAAGGCCAAACGCGCTGTCGTTTCCCTTGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTc                                      <  8:123922/149‑1 (MQ=255)

                                                                                                        aCTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTc                                      <  3:343502/149‑1 (MQ=255)

                                                                                                        aCTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTc                                      <  7:43000/149‑1 (MQ=255)

                                                                                                         cTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTAtt                                       >  3:242671/1‑147 (MQ=255)

                                                                                                         cTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCa                                     <  2:190199/149‑1 (MQ=255)

                                                                                                          tCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAg                                    >  8:62891/1‑149 (MQ=255)

                                                                                                            aGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGATGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTc                                      <  3:359583/145‑1 (MQ=255)

                                                                                                            aGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGATGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTc                                      >  4:359583/1‑145 (MQ=255)

                                                                                                            aGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATa                                                     >  6:295436/1‑130 (MQ=255)

                                                                                                            aGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATa                                                     <  5:295436/130‑1 (MQ=255)

                                                                                                             gTGCGCTGGTGAATCGCGAACTCA‑‑CAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggtggt                               <  3:272591/149‑1 (MQ=255)

                                                                                                                       gAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggtg                                 <  8:429697/139‑1 (MQ=255)

                                                                                                                       gAATCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggtg                                 >  7:429697/1‑139 (MQ=255)

                                                                                                                        aaTCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCaccacc                      <  5:87414/149‑1 (MQ=255)

                                                                                                                         aTCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCg                     <  4:242671/149‑1 (MQ=255)

                                                                                                                         aTCGCGAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCg                     <  7:438723/149‑1 (MQ=255)

                                                                                                                          tCGCGCACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAACACTGTATTCAGGTGGTGCCCCCACCgg                    >  5:386452/1‑149 (MQ=255)

                                                                                                                            gcgAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGt                   <  1:112599/148‑1 (MQ=255)

                                                                                                                             cgAACTCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTg                  >  4:206531/1‑148 (MQ=255)

                                                                                                                                  tCACCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGc            >  4:351862/1‑149 (MQ=255)

                                                                                                                                    aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGcca                           <  5:25245/132‑1 (MQ=255)

                                                                                                                                    aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa          >  8:155374/1‑149 (MQ=255)

                                                                                                                                    aCCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGcc           <  2:101659/148‑1 (MQ=255)

                                                                                                                                    aCCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGc            <  7:310518/147‑1 (MQ=255)

                                                                                                                                    aCCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGc            <  4:394080/147‑1 (MQ=255)

                                                                                                                                    aCCCAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGGTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa          <  1:279365/149‑1 (MQ=255)

                                                                                                                                     cccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGc            >  1:72718/1‑146 (MQ=255)

                                                                                                                                      ccAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaa                                                <  2:51649/109‑1 (MQ=255)

                                                                                                                                      ccAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaa                                                >  1:51649/1‑109 (MQ=255)

                                                                                                                                      ccAGCCGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCaga        <  3:46713/149‑1 (MQ=255)

                                                                                                                                          ccGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTAc    <  5:75056/149‑1 (MQ=255)

                                                                                                                                          ccGGACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTAc    >  5:398859/1‑149 (MQ=255)

                                                                                                                                            ggACAATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCg  >  7:277245/1‑149 (MQ=255)

GCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCG  >  NC_000913/1806835‑1807123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`