Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,115,547 A→G 5.0% L117P (CTG→CCG)  wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,115,5470AG 5.0% 251.9 / 2.5 79L117P (CTG→CCG) wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (39/36);  new base G (2/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

GCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATTGT  >  NC_000913/2115400‑2115694
                                                                                                                                                   |                                                                                                                                                   
gCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCa                                                                                                                                                     <  6:154648/148‑1 (MQ=255)
gCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAg                                                                                                                                                    <  6:251670/149‑1 (MQ=255)
 cGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAgg                                                                                                                                                   <  8:55278/149‑1 (MQ=255)
 cGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAgg                                                                                                                                                   >  7:235086/1‑149 (MQ=255)
  gTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATGAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAggg                                                                                                                                                  <  5:104640/149‑1 (MQ=255)
  gTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATTGTGCAGTCAGAACCACGCACATCCAggg                                                                                                                                                  <  5:263330/149‑1 (MQ=255)
  gTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAggg                                                                                                                                                  >  5:108274/1‑149 (MQ=255)
     aTTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGt                                                                                                                                                 >  3:33162/1‑147 (MQ=255)
     aTTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGGCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGa                                                                                                                                               <  1:246746/149‑1 (MQ=255)
         gCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGtt                                                                                                                                           <  1:373775/149‑1 (MQ=255)
              gCTAACGGTCATGTTTTCTATGATCAGGTTTCTCATGACCCGTTTTTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGc                                                                                                                                       <  6:354169/148‑1 (MQ=255)
                tAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCGGGGTGATGTTATGCATa                                                                                                                                    >  7:158537/1‑149 (MQ=255)
                   cGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTg                                                                                                                                 <  5:73572/149‑1 (MQ=255)
                    ggTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCAGGGTGATGTTATGCATACTgcc                                                                                                                               >  7:138795/1‑149 (MQ=255)
                      tCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTgccg                                                                                                                              <  8:235086/149‑1 (MQ=255)
                      tCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTACAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTgccg                                                                                                                              >  1:95477/1‑149 (MQ=255)
                              tCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAg                                                                                                                                                    >  7:141174/1‑119 (MQ=255)
                              tCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAg                                                                                                                                                    <  8:141174/119‑1 (MQ=255)
                               cGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCAtcctcc                                                                                                                     >  7:340763/1‑149 (MQ=255)
                               cGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCAtcctcc                                                                                                                     >  6:34121/1‑149 (MQ=255)
                                gatgatCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTccc                                                                                                                    <  5:146197/149‑1 (MQ=255)
                                      cAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATg                                                                                                                                        >  6:302661/1‑123 (MQ=255)
                                      cAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATg                                                                                                                                        <  5:302661/123‑1 (MQ=255)
                                                aTGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGcc                                                                                                    >  5:355619/1‑149 (MQ=255)
                                                 tGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCg                                                                                                   >  7:307078/1‑149 (MQ=255)
                                                   aCCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTACAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTc                                                                                                 <  2:95477/149‑1 (MQ=255)
                                                      cGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTg                                                                                              <  4:345839/149‑1 (MQ=255)
                                                       gtttgtttTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTa                                                                                            <  8:138795/149‑1 (MQ=255)
                                                            tttttGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCGGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGAc                                                                                        <  8:158537/149‑1 (MQ=255)
                                                            tttttGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATa                                                                                                                                    >  5:145942/1‑105 (MQ=255)
                                                            tttttGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATa                                                                                                                                    <  6:145942/105‑1 (MQ=255)
                                                            tttttGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGAc                                                                                        <  8:212738/149‑1 (MQ=255)
                                                                    gccgATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGcc                                                                                <  4:33162/149‑1 (MQ=255)
                                                                    gccgATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGcc                                                                                <  8:307078/149‑1 (MQ=255)
                                                                     ccgATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGccc                                                                               >  7:237846/1‑149 (MQ=255)
                                                                       gATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCctc                                                                             <  6:31535/149‑1 (MQ=255)
                                                                             aaTCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCAcc                                                                                                            <  5:201077/112‑1 (MQ=255)
                                                                             aaTCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCAcc                                                                                                            >  6:201077/1‑112 (MQ=255)
                                                                              aTCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGtt                                                                      >  1:193130/1‑149 (MQ=255)
                                                                               tCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTg                                                                     >  8:434469/1‑149 (MQ=255)
                                                                               tCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTg                                                                     >  3:396671/1‑149 (MQ=255)
                                                                                   cGTCACCGGGCCAAAGCCGCGCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTg                                                                     <  4:7139/145‑1 (MQ=255)
                                                                                     tCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCcgcg                                                                >  6:64841/1‑148 (MQ=255)
                                                                                        ccGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAg                                                            >  5:51281/1‑149 (MQ=255)
                                                                                         cGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGc                                                           >  4:401147/1‑149 (MQ=255)
                                                                                           ggCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCtt                                                         >  6:217751/1‑149 (MQ=255)
                                                                                                aaGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTcc                                                       >  8:164945/1‑146 (MQ=255)
                                                                                                 aGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAg                                                    >  1:27188/1‑148 (MQ=255)
                                                                                                   ccGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAAt                                                 <  5:34121/149‑1 (MQ=255)
                                                                                                     gCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGAc                                                                                        <  6:163994/108‑1 (MQ=255)
                                                                                                     gCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGAc                                                                                        >  5:163994/1‑108 (MQ=255)
                                                                                                       tCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCGGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAAtgtg                                              >  5:183335/1‑148 (MQ=255)
                                                                                                               aGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGttt                                      >  6:61473/1‑148 (MQ=255)
                                                                                                               aGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGttt                                      >  1:428457/1‑148 (MQ=255)
                                                                                                                 cccTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTAc                                   >  3:352199/1‑149 (MQ=255)
                                                                                                                 cccTTAGATGGTGCAGTCAGAACCACGCACATCCAGGCAGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCAGTAACACGAATAGCCCTATGCCGTTGCCGCGCAAGCTTCAAAGAAAGTGTAACTTTTTAc                                   >  8:407149/1‑149 (MQ=255)
                                                                                                                   cTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCg                                 >  2:237369/1‑149 (MQ=255)
                                                                                                                        aTGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCgggagg                            <  4:352199/149‑1 (MQ=255)
                                                                                                                         tGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCgggaggg                           <  5:61473/149‑1 (MQ=255)
                                                                                                                           gTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGAt                         <  5:64841/149‑1 (MQ=255)
                                                                                                                              cAGTCAGAACCACGCACATCCGGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATaa                       <  6:183335/148‑1 (MQ=255)
                                                                                                                               aGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAg                     <  1:237369/149‑1 (MQ=255)
                                                                                                                                gTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGa                    <  6:355619/149‑1 (MQ=255)
                                                                                                                                 tCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGAt                   <  5:217751/149‑1 (MQ=255)
                                                                                                                                 tCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGAt                   >  3:236997/1‑149 (MQ=255)
                                                                                                                                  cAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAAGGTGTAACGTTTTACCGGGAGGGATAAAGATg                  >  1:222043/1‑149 (MQ=255)
                                                                                                                                    gAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGTATAAAGATGcc                <  3:401147/149‑1 (MQ=255)
                                                                                                                                       ccACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGgt             <  6:51281/149‑1 (MQ=255)
                                                                                                                                       ccACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTAACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCAGgt             >  5:343367/1‑149 (MQ=255)
                                                                                                                                        cACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGgtg            >  3:153569/1‑149 (MQ=255)
                                                                                                                                         aCGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTAc                                   >  2:345170/1‑125 (MQ=255)
                                                                                                                                         aCGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTAc                                   <  1:345170/125‑1 (MQ=255)
                                                                                                                                          cGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGtt          <  6:108274/149‑1 (MQ=255)
                                                                                                                                          cGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGtt          >  1:105863/1‑149 (MQ=255)
                                                                                                                                           gCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGtt          >  2:243977/1‑148 (MQ=255)
                                                                                                                                           gCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTg         <  7:434469/149‑1 (MQ=255)
                                                                                                                                             acaTCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGa        >  5:252370/1‑148 (MQ=255)
                                                                                                                                               aTCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGatat     >  1:295243/1‑149 (MQ=255)
                                                                                                                                                 ccAGGGTGATGTTATGCATACTGCCGCCCTCCTCCCCTGTCTCCTGGCTGCCGGCCTGTAAGACTAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGatat     <  1:150031/147‑1 (MQ=255)
                                                                                                                                                  cAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTTTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATTGt  >  6:325156/1‑149 (MQ=255)
                                                                                                                                                   aGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATTGt  >  4:191084/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATATAAATCTGCGTCACCGGGCCAAAGCCGCTCATAGTCAGCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATTGT  >  NC_000913/2115400‑2115694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: