Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,560,985 C→A 22.5% intergenic (+87/‑383) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,560,9850CA22.5% 108.3 / 9.9 65intergenic (+87/‑383)yffL/yffMCPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (25/24);  new base A (11/5);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCGGCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAA  >  NC_000913/2560837‑2561125
                                                                                                                                                    |                                                                                                                                             
gATCGGCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTc                                                                                                                                               <  8:191677/149‑1 (MQ=255)
    ggCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATTAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCaaaa                                                                                                                                           <  3:5433/149‑1 (MQ=255)
     gCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTCGGTCAAAAGTTGCCGTTACTGGTGGGTAAAAAg                                                                                                                                          >  1:257371/1‑149 (MQ=255)
      cAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGt                                                                                                                                         >  8:251890/1‑149 (MQ=255)
      cAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGtt                                                                                                                                        >  7:331972/1‑149 (MQ=255)
      cAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGtt                                                                                                                                        >  7:326029/1‑149 (MQ=255)
         aaaGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGc                                                                                                                                      <  7:212818/149‑1 (MQ=255)
              gAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTa                                                                                                                                 <  6:230846/149‑1 (MQ=255)
               aGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTaa                                                                                                                                <  1:177821/149‑1 (MQ=255)
               aGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGAGCTGGGGCAAAGGTTGCGGtg                                                                                                                                  >  5:381443/1‑146 (MQ=255)
                 tGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGTTGGGTAAAAAGTTGACTTTAAgg                                                                                                                              >  1:188193/1‑149 (MQ=255)
                 tGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAgg                                                                                                                              >  8:221023/1‑149 (MQ=255)
                   cAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGCGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGATATTGATGTGTAAAAATATGCAGTTAAgt                                                                                                                              >  2:272947/1‑146 (MQ=255)
                    aaCGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTgg                                                                                                                           <  6:411401/149‑1 (MQ=255)
                    aaCGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTgg                                                                                                                           >  5:411401/1‑149 (MQ=255)
                    aaCGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGCTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGCTTCAAAAGTTGACGTTAAGGTg                                                                                                                            >  6:116253/1‑148 (MQ=255)
                      cGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTATTGGTGGGTCAAAAGATGCCTTTAAggagtg                                                                                                                          >  8:85253/1‑144 (MQ=255)
                         aTACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAATTTGCGGTGTAGTTGGGTCa                                                                                                                       >  7:204607/1‑148 (MQ=255)
                          tACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTTATGTTGGGTCAAATTTTGCCGTTAAGGTGGGGCaaa                                                                                                                     >  5:20800/1‑149 (MQ=255)
                            ccAGTTATTATTCAATCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATTAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCTGTTAAGGTGGGTCaaaa                                                                                                                    <  3:344547/148‑1 (MQ=255)
                            ccAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAg                                                                                                                  >  3:225628/1‑149 (MQ=255)
                             cAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTATTGGTGGGTCAAAAGTTGCCGTTATGGTGGGTCAAAAGt                                                                                                                 >  5:238110/1‑149 (MQ=255)
                             cAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGTTGTGTAAAAATTTGACGTTAAGTTGGCTAAAATGt                                                                                                                 >  5:25858/1‑149 (MQ=255)
                               gTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTg                                                                                                               <  7:221023/149‑1 (MQ=255)
                                  attattCAATCCGTACAACATGAAATAACGGAATCCGTCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGcc                                                                                                             <  4:225628/148‑1 (MQ=255)
                                  attattCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGTTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCg                                                                                                            >  5:52014/1‑149 (MQ=255)
                                     attCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTgg                                                                                                                           <  7:240296/132‑1 (MQ=255)
                                     attCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTgg                                                                                                                           >  8:240296/1‑132 (MQ=255)
                                     aatCAAGATGTACATCTTGAAATAAAGGACTCTGTTCGGCAATGTTAAGTCATTTTCTTTTCCTTTATTCCATGTATTCAGGTGGGTAAAAAGTTGGCGTTATTGTTGGGTAAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGtt                                                                                                          <  6:52014/146‑1 (MQ=12)
                                      ttCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGACGtt                                                                                                          >  7:312791/1‑147 (MQ=255)
                                       tCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTTATGATGGGTAAAAATTTGAGGTTAAGTTAGGTACTAAGTTGccaatatt                                                                                                       >  6:286746/1‑143 (MQ=255)
                                         aaGCCGTACAACATGAAATAACGGAATCAGCCGTGCAATGCTCACGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGGCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAgg                                                                                                      <  5:116253/148‑1 (MQ=255)
                                               tACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTTGTGGTGGGTAAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGACGTTAAGGTgtggc                                                                                                >  3:122355/1‑144 (MQ=255)
                                                 caacaTGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTATTGGTGGGTCAAAAGTTGCAGTTTAGGATGGTCTAAAGTTGCAGTGAATGTCGGTaaa                                                                                              >  3:244658/1‑148 (MQ=255)
                                                    caTGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTATTGTTGGGTAAAAAGTTGCCGTTAAGGTGTGTCAAAATTTTAAGTAAATGAGGGTCaaaatt                                                                                          >  7:143089/1‑147 (MQ=255)
                                                       gAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGGGGGTAAAAAGTTGCCGTTAAGGGGGGGGAAAAGTTGCAGTATAGGTGGGGCTAAATTTga                                                                                       >  6:194906/1‑148 (MQ=255)
                                                       gAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGATAATGGTGGGTAAAAATATGCCGTTAAGGTGGGGCAAATTTTGAAGTTAACGTGTGAGAAAAGTTg                                                                                        >  1:164437/1‑148 (MQ=255)
                                                          aTAACGGAATCCGTCGGGCATTTATCCAGCCTTTTGTATGCCATAAATAAATGAATTCAGGGGGGAAAAAATATGCCGTTAGTGGTGGGTCAAAAGTTGCCTTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGa                                                                                    <  8:76655/149‑1 (MQ=18)
                                                                  atacgcagggCATTTTTGCAGCCTTTTCTATCCCATTATTAAATGAATTCAGGTGGGTTAAAAGTTGCCGTTAGTGGTGGGTCATTAGATGCCTTTTAGGTGGGTCAAAAGTTTCCTTTAAGGTGGGTCAAAAGTTGCCGAGTAAGaa                                                                             <  4:218419/141‑1 (MQ=19)
                                                                  aTCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAAt                                                                            <  7:107424/149‑1 (MQ=255)
                                                                          ggCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCaaaa                                                                                                                    <  1:340850/102‑1 (MQ=255)
                                                                          ggCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCaaaa                                                                                                                    >  2:340850/1‑102 (MQ=255)
                                                                           gCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTAAAATTTTGCCGTTTAGGTGGGTCAAAATTTGGAGTTAAGGTGTGTGTAATTTTGACGAGTAAGAATTCTACAAt                                                                    >  7:429969/1‑148 (MQ=25)
                                                                             aaTGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGATGCCGTTAGTGGGTGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTaa                                                                 <  7:197620/149‑1 (MQ=255)
                                                                               tGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTATTGGTGGGTCAAAATTTGCCTTTAAGGTGGGTCAAAAGTTGAAGTTAAGGTGTGGCAAAATTTTACTAGTAAGAATTCTAATATTatt                                                                >  8:315579/1‑146 (MQ=25)
                                                                               tGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCaaaa                                                                                                                    <  2:369083/97‑1 (MQ=255)
                                                                               tGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCaaaa                                                                                                                    >  1:369083/1‑97 (MQ=255)
                                                                                    cagCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGTGTAAAAAGTTGCCGTTAGTGGTGGGTAAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCt                                                          <  3:47580/147‑1 (MQ=255)
                                                                                      gCCTTTTCATTGCCTAAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTgg                                                                                                   <  3:218257/106‑1 (MQ=255)
                                                                                              tttGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAATGTTGGGTCAAAATTTGCCGTTAAGGAGTGTCAAATTTTGAAGTAAACGTGTCTCAAAATTTGACAACTAAAAATACTAGTATTGAGAATACTAGGctagtg                                                 >  8:109374/1‑143 (MQ=16)
                                                                                                    ttAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTTTTGGTGGGTAAAAATTTGCAGTTAAGGGGTGTGAAAATTTTGAGGTTAAGGGGGGGAAAATTTTAGGAGTAAAGATTGTAAAATTAAGAATTAAAGGGGATGGGAGttta                                          >  2:201306/1‑148 (MQ=255)
                                                                                                       aattcATGAATTCAGGTGGGCCCAAATTTGCCGTTATTGGTGGCTAAAAAGTTGCCTTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTa                                       <  8:312791/145‑1 (MQ=37)
                                                                                                         tCCAGGAATTCCGGGGGGCCCAAAGAAGCCCTTAGGGGTGGGTCAAAAGCGGCCGTTCCGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGc                                     <  7:251890/149‑1 (MQ=25)
                                                                                                               aaTTCAGGTGGCTAAAAATTTGGGTTTCTGGGTGGGTCAAATCTTGCCGTTAAGGTGGGTCAAATGTTTCATTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATAAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTccc                                <  8:317555/148‑1 (MQ=25)
                                                                                                                          gTCAAAAGATGCCCGTAGTGGTGCCTCCACAGCAGCCGTGACGGGGTCCCAAATCTGCCCTTCAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATaga                   <  7:23711/149‑1 (MQ=255)
                                                                                                                           tCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTTAGGGGGGTCAAAAGTTTGCGTGAAGGTGGGGCAAAAGTTGCCTAGGAAGGAGTCTACAATTAATAATTATACGCGACGTGAGTGTTTTTGCGTAAcggtaggtgaaa                     >  8:428735/1‑143 (MQ=16)
                                                                                                                           tCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTTGGGAAAAATTTGCCGTTAAGGTGGGTGAAATGTTTGTGAGTAAGATTTGTACAATTAAGAATACTAGGCGACTGGAGTTTTGTAGCCTAGCGGTCGGTCa                       >  2:161769/1‑145 (MQ=25)
                                                                                                                           tCAAAAGCTGCCGTTAGTGGTGGGTCAAAAGGAGCCGTTAAGGTGGGTCAAAAGTTGCCCTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATaga                   <  7:85253/149‑1 (MQ=255)
                                                                                                                           gcAAAAGTTGCCGTTAGTGTTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGATGGGAGTTTTTTAGCCTCCCGGTCGGTCATaga                   <  7:109374/148‑1 (MQ=255)
                                                                                                                                      ccttAGTGGTGGGTCAAAAGTTGCCGTAAAGGTGGGTCAAAAGGTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATagaga                 <  7:344057/138‑1 (MQ=255)
                                                                                                                                      cGTTAGTGGTGGGTCAAAAGTTGCCGTTTAGGTGGGTAAAAAGTTGCAGTTAAAGTGGGTCGAAAGTTGGAGAGTAAGCATTCTTCAATTTAGAATTCTAGGATACGGGACTTTTTTTGCCTCCCGGTCGGTAATagaga                 >  8:344057/1‑140 (MQ=25)
                                                                                                                                      cGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGcc           >  7:406915/1‑146 (MQ=255)
                                                                                                                                       gTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTa         >  4:219791/1‑147 (MQ=255)
                                                                                                                                       gTTAGGGTTGGTTCAAACCCTGCCGTTAAGTTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTaga       <  7:18984/149‑1 (MQ=38)
                                                                                                                                       cttcgtggtgGGTAAATAGTTGCGGTTAAGGTGGGTCAAAAGTTGCCGTTCAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTaga       <  3:48564/145‑1 (MQ=38)
                                                                                                                                            tggtggGTATATAGTAGCCGTTTAGTTGGGTCAATAGTTTCCTTTAAGGTGGGTCATAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCTTAGAGAGTCGCCTAGAGTTaa  <  6:383304/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GATCGGCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAA  >  NC_000913/2560837‑2561125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: