New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 248798229 (0.840)5 (0.160) 5/238 3.0 15.2% intergenic (+15/+41) evgS/yfdE hybrid sensory histidine kinase in two‑component regulatory system with EvgA/acetyl‑CoA:oxalate CoA‑transferase
?NC_000913 = 2487991 29 (0.910)intergenic (+24/+32) evgS/yfdE hybrid sensory histidine kinase in two‑component regulatory system with EvgA/acetyl‑CoA:oxalate CoA‑transferase
Rejected: Frequency below/above cutoff threshold.

TTAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCA  >  NC_000913/2487845‑2488135
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ttAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAAtg                                                                                                                                                <  5:20713‑M2/149‑3 (MQ=255)
             cTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTa                                                                                                                                   >  4:69035/1‑149 (MQ=255)
             cTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTa                                                                                                                                   >  3:15886/1‑149 (MQ=255)
                    aGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAAtgtg                                                                                                                                              <  5:41941‑M2/131‑5 (MQ=255)
                    aGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAAtgtg                                                                                                                                              >  6:41941‑M2/1‑127 (MQ=255)
                                  cTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTatg                                                                                                              <  6:20713/149‑1 (MQ=255)
                                   tGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTatga                                                                                                             >  4:105211/1‑149 (MQ=255)
                                   tGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTatga                                                                                                             >  2:187844/1‑149 (MQ=255)
                                     aaaTTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTatgat                                                                                                            <  1:193201/148‑1 (MQ=255)
                                     aaaTTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTatgat                                                                                                            <  3:69035/148‑1 (MQ=255)
                                                             tAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTc                                                                                     <  4:15886/147‑1 (MQ=255)
                                                                aaGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATg                                                                               >  1:118287/1‑149 (MQ=255)
                                                                     cacaTTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTcc                                                                                    >  6:153463/1‑140 (MQ=255)
                                                                     cacaTTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTcc                                                                                    <  5:153463/140‑1 (MQ=255)
                                                                      acaTTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGt                                                                          <  1:187844/149‑1 (MQ=255)
                                                                      acaTTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTc                                                                         <  2:118287/149‑1 (MQ=255)
                                                                         ttGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTc                                                                         >  1:185055/1‑147 (MQ=255)
                                                                                  tGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCg                                                              >  7:59151/1‑149 (MQ=255)
                                                                                       cAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTccc                                                         >  3:18083/1‑149 (MQ=255)
                                                                                         ggAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCgg                                                       <  3:105211/149‑1 (MQ=255)
                                                                                                           tCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAAtgtgggagccgctatttagtcatttttctgacagaaaacagcaatctcctggtccagctctgcaatgtgttcttttacagagttcagcaactgaagaatttcaggctta                                     <  5:30103‑M2/149‑110 (MQ=255)
                                                                                                                        tAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAtt                        >  4:7313/1‑149 (MQ=255)
                                                                                                                          aaTAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAtttt                      >  1:176305/1‑149 (MQ=255)
                                                                                                                                 gCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGa               <  3:7313/149‑1 (MQ=255)
                                                                                                                                 gCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGa               <  2:185055/149‑1 (MQ=255)
                                                                                                                                   tCCCACAATGTTCAAAtgtgggagccgctatttagtcatttttctgacagaaaacagcaatctcctggtccag                                                                                         >  2:194501‑M2/1‑16 (MQ=255)
                                                                                                                                   tCCCACAATGTTCAAAtgtgggagccgctatttagtcatttttctgacagaaaacagcaatctcctggtccag                                                                                         <  1:194501‑M2/73‑58 (MQ=255)
                                                                                                                                   tCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGAt              >  1:30825/1‑148 (MQ=255)
                                                                                                                                    cccACAATGTTCAAAtgtgggagccgctatttagtcatttttctgacagaaaacagcaatctcctggtccagctctgcaatgtgttcttttacagagttcagcaactgaagaatttcaggcttactgtcatctgaaa                        <  2:26727‑M2/137‑123 (MQ=255)
                                                                                                                                    cccACAATGTTCAAAtgtgggagccgctatttagtcatttttctgacagaaaacagcaatctcctggtccagctctgcaatgtgttcttttacagagttcagcaactgaagaatttcaggcttactgtcatctgaaa                        >  1:26727‑M2/1‑15 (MQ=255)
                                                                                                                                     ccACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCAt                                                                                >  4:55377/1‑80 (MQ=255)
                                                                                                                                     ccACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCAt                                                                                <  3:55377/80‑1 (MQ=255)
                                                                                                                                      cacaATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGc                                                                <  3:95047/95‑1 (MQ=255)
                                                                                                                                      cacaATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGc                                                                >  4:93035/1‑95 (MQ=255)
                                                                                                                                      cacaATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGc                                                                >  4:95047/1‑95 (MQ=255)
                                                                                                                                      cacaATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGc                                                                <  3:93035/95‑1 (MQ=255)
                                                                                                                                              tCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGcatca  >  8:6858/1‑149 (MQ=255)
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TTAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCA  >  NC_000913/2487845‑2488135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.