New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4347375113 (1.290)4 (0.050) 4/244 NT 3.6% intergenic (‑49/+29) fumB/dcuB anaerobic class I fumarate hydratase (fumarase B)/C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor
?NC_000913 = 4347385 111 (1.360)intergenic (‑59/+19) fumB/dcuB anaerobic class I fumarate hydratase (fumarase B)/C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor
Rejected: Frequency below/above cutoff threshold.

CGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4347221‑4347375
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgtgccgcCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCTGGAAGCTATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTACGT  <  NC_000913/4347385‑4347241
                                                                                                                                                                                                                                                                                                            
CGTCGAAGTCGGAAACGCTAACGTAATCGGAAGTGAGTAGATAGTTTTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCG                                                                                                                                                         <  3:169934/149‑1
 ggCGAAGGCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGT                                                                                                                                                        <  6:282698/147‑1
    GAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCC                                                                                                                                                     >  7:256294/1‑149
                                  GAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCC                                                                                                                                                     >  6:61645/1‑119
                                  GAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCC                                                                                                                                                     <  5:61645/119‑1
                                              TTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGC                                                                                                                                                   <  3:221234/109‑1
                                              TTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGC                                                                                                                                                   >  4:221234/1‑109
                                                                     GGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATA                                                                                                             >  7:215399/1‑124
                                                                     GGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATA                                                                                                             <  8:215399/124‑1
                                                                                                                                           GAAAATGGCGTGCCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCTGGAAGCTATGTCAAACAAACCCTTT                                                                      <  4:241612/93‑1
                                                                                                                                           GAAAATGGCGTGCCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCGTGAAGCTATGGCAAACAAACCCTTT                                                                      >  3:241612/1‑93
                                                                                                                                                   CGTGCCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCTGGAAGCTATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGAT       <  4:35588/148‑1
                                                                                                                                                   CGTGCCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCTGGAAGCTATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGAT       >  6:362386/1‑148
                                                                                                                                                   CGTGCCGCCAGTGACTACGCGGCATGCCATTTTCGAATAACAAATACAGAGTTACAGGCTTTAAGCTATGTCAACCAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATT      <  4:233591/149‑1
                                                                                                                                                    GTGCCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCTGGAAGCTATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTA     >  5:215059/1‑149
                                                                                                                                                    GTGCCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCTGGAAGCTATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTA     >  2:383953/1‑149
                                                                                                                                                     TGCCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCTGGAAGCTATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTAC    <  7:303456/149‑1
                                                                                                                                                     TGCCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCTGGAAGCTATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTAC    <  1:100100/149‑1
                                                                                                                                                       CCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCTGGAAGCTATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTACGT  <  1:186342/149‑1
                                                                                                                                                       CCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCTGGAAGCTATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTACG   <  5:213809/148‑1
                                                                                                                                                        CGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACATATTTACAGGCTGGAAGCTATGTCAAACAAACCCTTTATCTACAAGGCACCTTTCCCGATGTTGAAAGACAATACCGAATACTATCTACTCACTTCCGATTACGT  >  6:243079/1‑148
                                                                                                                                                        CGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCTGGAAGCTATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTACGT  >  8:81195/1‑148
                                                                                                                                                                                                                                                                                                            
CGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4347221‑4347375
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgtgccgcCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCTGGAAGCTATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTACGT  <  NC_000913/4347385‑4347241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.