New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4353155 =	140 (1.600)	4 (0.050)	4/232	NT	3.4%	intergenic (+74/+45)	ghoT/lysU	toxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible
	?	NC_000913	4353189 =	104 (1.350)					intergenic (+108/+11)	ghoT/lysU	toxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible
Rejected: Frequency below/above cutoff threshold.

GTCTGCCGCCAACCGCTGGTCTGGGTATTGGTATCGACCGAATGATTATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCGTTCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4353315‑4353155 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gactgcttcgttcatTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACG  >  NC_000913/4353189‑4353335                                                                                                                                                                                                                                                                                                                      GTCTGCCGCCAACCGCTGGTCTGGGTATTGGTATCGACCGAATGATTATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGAC                                                                                                                                                                 <  8:134124/149‑1  TCTGCCGCCAACCGCTGGTCTGGGTATTGGTATCGACCGAATGATTATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACT                                                                                                                                                                <  7:327761/149‑1     GCCGCCAACCGCTGGTCTGGGTATTGGTATCGACCGAATGATTATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCT                                                                                                                                                             >  5:16737/1‑149       CGCCAACCGCTGGTCTGGGTATTGGTATCGACCGAATGATTATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTC                                                                                                                                                           <  1:202481/149‑1       CGCCAACCGCTGGTCTGGGTATTGGTATCGACCGAATGATTATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTC                                                                                                                                                           <  3:280135/149‑1        GCCAACCGCTGGTCTGGGTATTGGTATCGACCGAATGATTATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCG                                                                                                                                                          >  8:205582/1‑149         CCAACCGCTGGTCTGGGTATTGGTATCGACCGAATGATTATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCGT                                                                                                                                                         >  4:350554/1‑149         CCAACCGCTGGTCTGGGTATTGGTATCGACCGAATGATTATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCGT                                                                                                                                                         <  4:209220/149‑1         CCAACCGCTGGTCTGGGTATTGGTATCGACCGAATGATTATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCGT                                                                                                                                                         >  1:253420/1‑149           AACCGCTGGTCTGGGTATTGGTATCGACCGAATGATTATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCGTTC                                                                                                                                                       <  4:75225/149‑1             CCGCTGGTCTGGGTATTGGTATCGACCGAATGATTATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCGTTCAT                                                                                                                                                     >  7:142180/1‑149                                               TATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCGT                                                                                                                                                         >  8:259651/1‑111                                               TATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCGT                                                                                                                                                         <  7:259651/111‑1                                                                                      TCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGC                                                                                                                            >  5:131509/1‑101                                                                                      TCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGC                                                                                                                            <  6:131509/101‑1                                                                                                       GCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGCAGCGAATAACGTCGCGAATAGTATGGCGGTTATTAAACCTCATAATCA                                                                >  2:105601/1‑144                                                                                                       GCCCAAAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGAGCATCGCCGTGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCA                                                                <  1:105601/144‑1                                                                                                                                                   GACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTC               >  7:9365/1‑149                                                                                                                                                   GACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTC               <  7:400938/149‑1                                                                                                                                                     CTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCCGCGGTTGGCGGCAGACCATATTCCA             <  5:19313/149‑1                                                                                                                                                       GCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGC           >  1:155347/1‑149                                                                                                                                                        CTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCG          >  8:119952/1‑149                                                                                                                                                        CTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGC           <  6:110773/148‑1                                                                                                                                                         TTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCG          >  7:105850/1‑148                                                                                                                                                            GTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGT      >  8:58813/1‑149                                                                                                                                                                ATTAAAGTGAAATTTATTTGTGTGGGCGCATCGCCGGGAAGAGAATACCGTCGCGAATAGTATGGCTGGTAGTAAACAGCATAATCATTCGGTCGATACCAATACGCAGACCAGCGGTTGGCGGCAGCCCCTTTTCCAGCGCAGTCAgg  >  2:106630/1‑147                                                                                                                                                                ATTAAAGGGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCAC   <  4:272788/148‑1                                                                                                                                                                 TTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCCGCCCATATTCCAGCGCAGTCACG  >  4:147984/1‑148                                                                                                                                                                                                                                                                                                                      GTCTGCCGCCAACCGCTGGTCTGGGTATTGGTATCGACCGAATGATTATGCTGTTTACTAACAGCCATACTATTCGCGACGTTATTCTCTTCCCGGCGATGCGCCCACAGAAATAAATTTCACTTTAATGAACGAAGCAGTCAGGCGACTGCTTCGTTCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4353315‑4353155 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gactgcttcgttcatTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACG  >  NC_000913/4353189‑4353335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.