Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 819,973 T→A 6.0% V30V (GTT→GTA)  ybhL → putative acetate transporter; BAX Inhibitor‑1 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913819,9730TA 6.0% 206.8 / 3.3 67V30V (GTT→GTA) ybhLputative acetate transporter; BAX Inhibitor‑1 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (32/31);  new base A (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

GCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTATTGTGTTATCAGCGATGATTCAAAAG  >  NC_000913/819829‑820120
                                                                                                                                                |                                                                                                                                                   
gCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCt                                                                                                                                                 >  5:86971/1‑149 (MQ=255)
  cTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTAGGCttg                                                                                                                                               >  7:131388/1‑149 (MQ=255)
  cTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTAGGCttg                                                                                                                                               >  7:46967/1‑149 (MQ=255)
     atcatTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTATATGGCTGGATGACCGTTGGCttgttg                                                                                                                                            >  5:358103/1‑149 (MQ=255)
                 tATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCAttt                                                                                                                                >  8:225224/1‑149 (MQ=255)
                 tATCCTTAAGATATTCCTTATATATTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCTGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGGTTTTTGCTGACAGCAttt                                                                                                                                >  3:262444/1‑149 (MQ=255)
                  aTCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATttg                                                                                                                               >  4:58097/1‑149 (MQ=255)
                   tCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCTTGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCAAGGGCTGGATGACCGTTGGCATGTTGCTGACCGCATttgt                                                                                                                              >  2:211119/1‑149 (MQ=255)
                      ttAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGc                                                                                                                           >  6:180384/1‑149 (MQ=255)
                         aGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCt                                                                                                                         <  6:86971/148‑1 (MQ=255)
                                ccTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAGGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCg                                                                                                                 >  4:27040/1‑149 (MQ=255)
                                    atatCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGTCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCt                                                                                                              >  3:258198/1‑148 (MQ=255)
                                    atatCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGc                                                                                                               <  8:310506/147‑1 (MQ=255)
                                    atatCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGc                                                                                                               <  3:21371/147‑1 (MQ=255)
                                      atCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAAt                                                                                                           >  4:122101/1‑149 (MQ=255)
                                      atCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAAt                                                                                                           >  7:202119/1‑149 (MQ=255)
                                      atCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAAt                                                                                                           >  2:25736/1‑149 (MQ=255)
                                            aGGAGATCGTCATAGACAGATTCCCACGTTCTGATTCAAACCTACACCCCCGGGCTGGCTTGCAAACTTATATGGCTCAACTCTATGGCTGGATGACCGTTGGCTTTTAGCTGACCGCATTAGTTGCCTGGTATGCGGCTAATTCcgcg                                                                                                     >  8:104241/1‑149 (MQ=255)
                                              gagaTCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGc                                                                                                   >  4:336724/1‑149 (MQ=255)
                                                    gTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCAGTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGa                                                                                              <  6:41240/148‑1 (MQ=255)
                                                     tCATTGACAGATTCCCACGTTCTGATGCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGGTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATg                                                                                            <  2:276234/149‑1 (MQ=255)
                                                        tGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATttgtt                                                                                                                             <  4:336981/113‑1 (MQ=255)
                                                        tGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATttgtt                                                                                                                             >  3:336981/1‑113 (MQ=255)
                                                          gACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCt                                                                                       >  3:361042/1‑149 (MQ=255)
                                                           aCAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCtg                                                                                      >  1:95169/1‑149 (MQ=255)
                                                               aTTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAGGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCtgttgt                                                                                  <  3:27040/149‑1 (MQ=255)
                                                                ttCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTgttgtt                                                                                 <  4:361042/149‑1 (MQ=255)
                                                                ttCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTgttgtt                                                                                 <  8:399154/149‑1 (MQ=255)
                                                                  cccACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGa                                                                                          <  5:269124/138‑1 (MQ=255)
                                                                  cccACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGa                                                                                          >  6:269124/1‑138 (MQ=255)
                                                                   ccACGTTCTGATTCATTCGTACAAGCCCGGGCTGGCTTGCTAACTTATATGGCTCAAGGCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGACGTGATGGAGCTGTTGTTCa                                                                               <  2:349341/148‑1 (MQ=255)
                                                                   ccACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCAc                                                                              >  2:242305/1‑149 (MQ=255)
                                                                     aCGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCttgttg                                                                                                                                            <  2:337055/85‑1 (MQ=255)
                                                                     aCGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCttgttg                                                                                                                                            >  1:337055/1‑85 (MQ=255)
                                                                             aTTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCgtgt                                                                     <  8:202119/148‑1 (MQ=255)
                                                                             aTTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCgtg                                                                      <  2:7831/147‑1 (MQ=255)
                                                                               tCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCtt                                                                  >  1:46788/1‑149 (MQ=255)
                                                                                 caTCGTACAACCCCGGGCTTGCGGGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGGTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGATCTGTTGTTCACTAACCGTGTCtttt                                                                <  7:358153/148‑1 (MQ=255)
                                                                                 aaTCGTACCACCACGGGCTGGCGTGCAAACTTATATGGCGCAAGTGTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCAGTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCtttt                                                                <  7:363635/149‑1 (MQ=255)
                                                                                 aaTCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCtttt                                                                <  5:180384/149‑1 (MQ=255)
                                                                                     gTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTAGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAAt                                                            <  8:46967/149‑1 (MQ=255)
                                                                                     gTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTAGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAAt                                                            <  8:131388/149‑1 (MQ=255)
                                                                                      tACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATc                                                           >  5:271323/1‑149 (MQ=255)
                                                                                            cccGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCt                                                      >  2:13651/1‑148 (MQ=255)
                                                                                              cGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTgttgtt                                                                                 >  8:398252/1‑119 (MQ=255)
                                                                                              cGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTgttgtt                                                                                 <  7:398252/119‑1 (MQ=255)
                                                                                                  ctggctTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGatcatc                                               <  1:59338/149‑1 (MQ=255)
                                                                                                  ctggctTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGAGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGatcatc                                               <  7:104241/149‑1 (MQ=255)
                                                                                                           aaaCTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAAt                                        >  7:404551/1‑147 (MQ=255)
                                                                                                           aaaCTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTa                                      <  4:258198/149‑1 (MQ=255)
                                                                                                           aaaCTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTa                                      <  7:225224/149‑1 (MQ=255)
                                                                                                              cTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCa                                   <  6:271323/149‑1 (MQ=255)
                                                                                                                   atGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGATAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGt                              >  1:10925/1‑149 (MQ=255)
                                                                                                                      gCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTa                            <  7:55925/148‑1 (MQ=255)
                                                                                                                       cTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTAtt                          <  3:122101/149‑1 (MQ=255)
                                                                                                                        tCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTATtg                         >  6:309025/1‑149 (MQ=255)
                                                                                                                          aaGTCTATGGCTGGTTGACCGGTGGGTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTATtgt                        <  1:211119/148‑1 (MQ=255)
                                                                                                                          aaGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTATtgtg                       <  1:13651/149‑1 (MQ=255)
                                                                                                                          aaGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCAGTGGTTATtgtg                       >  1:390604/1‑149 (MQ=255)
                                                                                                                                 tGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTATTGTGTTATCAg                <  3:58097/149‑1 (MQ=255)
                                                                                                                                   gCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTATTGTGTTATCAGc               >  4:19276/1‑148 (MQ=255)
                                                                                                                                    cTGTATGACCGTTTGCTGTTTGCTGACCGCATTTTTAGCCTGGTATGCGGCTAATTCCGCGGACGTGATTGATCTGTTGTTCACTAACCGTGTCATTTTAATCGTTATGATCATCGCGCAATTAGCATTGGTTATTGTGTTATCAGCga             <  4:262444/149‑1 (MQ=255)
                                                                                                                                    cTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTATTGTGTTATCAGCga             >  4:174074/1‑149 (MQ=255)
                                                                                                                                    cTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTATTGTGTTATCAGCga             <  1:242305/149‑1 (MQ=255)
                                                                                                                                      ggATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTATTGTGTTATCAGCgat            >  7:29001/1‑148 (MQ=255)
                                                                                                                                         tGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTATTGTGTTATCAGCGATGAtt        <  5:309025/149‑1 (MQ=255)
                                                                                                                                             cGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATGAGCATTGGTTATTGTGTTATCAGCGATGAATCaa     >  8:176428/1‑148 (MQ=255)
                                                                                                                                               ttGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTGGGCGGACGTGATGGAGGTGTTGTTCAGTATGGGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCTTTGGTTATTGTGTTATCAGCGTTGATTcttaag  <  7:176428/149‑6 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTATTGTGTTATCAGCGATGATTCAAAAG  >  NC_000913/819829‑820120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑