Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,513,698 | C→T | 7.3% | N294N (AAC→AAT) | ydcT → | putative ABC transporter ATPase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,513,698 | 0 | C | T | 7.3% | 160.6 / 2.9 | 55 | N294N (AAC→AAT) | ydcT | putative ABC transporter ATPase |
Reads supporting (aligned to +/- strand): ref base C (20/30); new base T (2/2); total (22/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.06e-01 |
TGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGA > NC_000913/1513550‑1513840 | tGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAAc < 5:77773/149‑1 (MQ=255) ggCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTTAAGTGCAGGCCACTGTCACGATCCACGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGTAc < 8:384960/148‑1 (MQ=255) gCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAAcg < 1:298650/148‑1 (MQ=255) tgtgGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAAc < 7:101711/148‑1 (MQ=255) aTGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGGAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTtgt < 5:88762/149‑1 (MQ=255) aTGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGATATTGAACGGCGGTGAAAAACTGCTtgt < 5:344367/149‑1 (MQ=255) gACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTtgt > 8:291659/1‑147 (MQ=255) gACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTtgt > 3:372746/1‑147 (MQ=255) aCGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGa < 5:166356/148‑1 (MQ=255) aCGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGa < 3:87722/148‑1 (MQ=255) aCGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGa < 3:62151/148‑1 (MQ=255) ggAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCa < 7:291659/149‑1 (MQ=255) gAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGCACGGCGGTGAAAAACTGCTGGTGAGTCAg > 3:218756/1‑149 (MQ=255) gAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAg > 7:247063/1‑149 (MQ=255) aGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGACCTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGc < 3:93764/149‑1 (MQ=255) aGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGc < 8:377365/149‑1 (MQ=255) cTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCa < 8:223236/149‑1 (MQ=255) cTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCATGGTGAACTGCAGCCAAATGGCCCGATACAGGAGGTGCAATATCAGCGCGCGGCAACTCGTTTTGAACTGAAAAAGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGcc > 1:170420/1‑148 (MQ=255) cGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGGGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAat < 6:347432/148‑1 (MQ=255) cGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCAAGGCGGTGCAATATCAGGGCGCGGCCTCTCGTTTTGAAATGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAata > 2:191717/1‑149 (MQ=255) cccTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAatat > 3:119132/1‑148 (MQ=255) gCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCtt < 7:406446/127‑1 (MQ=255) cGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAg < 1:139765/148‑1 (MQ=255) aCCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAgg > 8:76393/1‑134 (MQ=255) aCCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAgg < 7:76393/134‑1 (MQ=255) ccGGAACATATCCGCCTCAACACCCCTGGTGCACTGCAGGCCAATGGCACGATCCAGGCGGAGCAATATCAGGGCGCGGCAACTCGTTGTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCg > 1:306205/1‑148 (MQ=255) cGGAACATATCCGCCTCAACACCCCTGGTTAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGTCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCgaa < 7:243007/149‑1 (MQ=255) cATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATTTCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAAct < 5:336884/149‑1 (MQ=255) ccTCAACTCCCCGGGCGACCTGCAGGCCAAGCGCACGAGCCAGGCGGAGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGCTGCAAAACTGCTTGTGAGTCAGGCGAATATGACAGGCGAAGCACTGCCTGCCa < 2:72742/149‑1 (MQ=255) cccTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAAATGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGc > 4:140968/1‑149 (MQ=255) ccTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGcc > 7:68000/1‑149 (MQ=255) cTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGccc > 4:270114/1‑149 (MQ=255) gTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCg < 6:128319/147‑1 (MQ=255) gTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGa < 8:264096/149‑1 (MQ=255) aaCTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGacaa > 3:84472/1‑149 (MQ=255) aCTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGacaac < 4:84472/149‑1 (MQ=255) cAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGTCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAgg < 5:151545/148‑1 (MQ=255) cAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGt > 6:88623/1‑149 (MQ=255) aGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAActgcc > 4:343075/1‑121 (MQ=255) tGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAATGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGttt > 7:45656/1‑149 (MQ=255) tGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAATGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGttt > 7:51855/1‑149 (MQ=255) gCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGATATTGAACGTCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGAGAAGAAGTGCCTGCCTCGCTCACGCCCGGACAACAGGTGATGGTTTc < 2:170420/148‑1 (MQ=255) gCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAATGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTc < 8:45656/148‑1 (MQ=255) gCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAATGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTc < 8:51855/148‑1 (MQ=255) cACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCt > 5:168302/1‑149 (MQ=255) aTCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGCCAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACCGGTGATGGTTTCCTGGTc > 4:301118/1‑149 (MQ=255) aGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTg < 5:101841/142‑1 (MQ=255) aGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTg > 6:101841/1‑142 (MQ=255) aaTATCAGGGCGCGGCAACGCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGtgatgtgatg < 3:37210/149‑1 (MQ=255) tCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCTGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAATAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTg < 2:20120/148‑1 (MQ=255) gggCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGc < 6:168302/149‑1 (MQ=255) gcgcGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTg > 3:195924/1‑149 (MQ=255) cgcgGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTgg < 3:143272/149‑1 (MQ=255) cAACTCGTTTTGTACTGAAATTGAAAGGAGGTGAAAAACTGCTTTTGAGTCAGGCCAATATGACAGGCGAATAACTGCCTGCCAAGCTCACGCCCTGACAACAGGGGATGGTTTCCTGGTCGCGTGATGTGAGGGTGCCGCTGGTTgag < 2:306205/149‑1 (MQ=255) ttttGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGGTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGt < 8:247063/149‑1 (MQ=255) aCTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATgg < 8:68000/149‑1 (MQ=255) cTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGc > 2:33572/1‑149 (MQ=255) aTTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGa < 4:372746/149‑1 (MQ=255) | TGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGA > NC_000913/1513550‑1513840 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |