Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,029,388 A→G 7.7% intergenic (‑22/‑151) yedI ← / → yedA DUF808 family inner membrane protein/amino acid exporter for phenylalanine, threonine

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,029,3880AG 7.7% 193.5 / 4.0 66intergenic (‑22/‑151)yedI/yedADUF808 family inner membrane protein/amino acid exporter for phenylalanine, threonine
Reads supporting (aligned to +/- strand):  ref base A (31/29);  new base G (3/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGAGG  >  NC_000913/2029241‑2029534
                                                                                                                                                   |                                                                                                                                                  
cGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                                                                                                   <  8:208084/149‑1 (MQ=255)
  aTAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAgg                                                                                                                                                 <  4:104655/149‑1 (MQ=255)
  aTAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAgg                                                                                                                                                 <  3:92868/149‑1 (MQ=255)
  aTAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAgg                                                                                                                                                 >  4:40602/1‑149 (MQ=255)
  aTAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAgg                                                                                                                                                 <  2:58666/149‑1 (MQ=255)
     aGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGACAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGgtg                                                                                                                                              >  5:48883/1‑149 (MQ=255)
      gTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGgtg                                                                                                                                              >  4:353265/1‑148 (MQ=255)
         aTCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAg                                                                                                                                          >  7:125459/1‑149 (MQ=255)
           ccccTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAgg                                                                                                                                                 >  8:330547/1‑140 (MQ=255)
           ccccTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTa                                                                                                                                        >  8:300217/1‑149 (MQ=255)
           ccccTAATACACCGGCGGGTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAgg                                                                                                                                                 <  7:330547/140‑1 (MQ=255)
               tAATACACCGGCGGTTTTGTTCGCCGCCAGTTTTCCCATCACGGAGATATCGTCCAGTAGTGTGGGGATATAATCGAGCAACGGCAGTAAACTACTCCCGGCCAAAAGCATTGCCGCGGATTTTTATTTTTGGAGGGGGTAAGTATg                                                                                                                                      >  6:382751/1‑147 (MQ=255)
                aaTACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAg                                                                                                                                   >  1:381516/1‑149 (MQ=255)
                 aTACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGaa                                                                                                                                    <  3:353265/147‑1 (MQ=255)
                    cacCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCaaa                                                                                                                               <  5:311564/149‑1 (MQ=255)
                     acCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCaaa                                                                                                                               >  8:335610/1‑148 (MQ=255)
                             ttttCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGGAGGGTGTAAGTATGAAGCAAAAACGGCAGc                                                                                                                      >  7:174391/1‑149 (MQ=255)
                             ttttCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGGAGGGTGTAAGTATGAAGCAAAAACGGCAGc                                                                                                                      >  7:179956/1‑149 (MQ=255)
                               ttcttcGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGctc                                                                                                                    >  6:35123/1‑149 (MQ=255)
                                       cgccAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACtt                                                                                                            >  1:268437/1‑149 (MQ=255)
                                                   cATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGt                                                                                                 >  2:106378/1‑148 (MQ=255)
                                                         ggAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCAATCACTCTCATTTTTATTGTTGAAGGGTGTAAGTATTAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACagt                                                                                          >  4:193130/1‑147 (MQ=255)
                                                          gagaTATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACAttt                                                                                         >  7:125443/1‑149 (MQ=255)
                                                            gaTATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCCGTAAACTACTCCCGGCCAAAAGTATTCCCTCTCAGTGTTATTTTTGTAGGGTGTAAGGATGAAGCATTAACGGCAGCTCTGATACTTGCTTCACAGGTCAACAttttt                                                                                       <  7:281914/149‑1 (MQ=255)
                                                            gaTATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACAttttt                                                                                       <  4:233164/149‑1 (MQ=255)
                                                             atatCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACAtttttt                                                                                      <  2:381516/149‑1 (MQ=255)
                                                                tCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTaa                                                                                   <  6:48883/149‑1 (MQ=255)
                                                                   tCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACaa                                                                                >  5:104706/1‑149 (MQ=255)
                                                                    ccAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAAt                                                                               >  1:90377/1‑149 (MQ=255)
                                                                       gtagtGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCaa                                                                            <  3:184415/149‑1 (MQ=255)
                                                                         agtGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGt                                                                          >  3:378254/1‑149 (MQ=255)
                                                                          gtgtGGCGATATCATCGAGCAACGTCAGGAAACTACTCCCGGACAAAATCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTg                                                                         <  6:213559/149‑1 (MQ=255)
                                                                            gtgGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGc                                                                        >  7:85155/1‑148 (MQ=255)
                                                                             tgGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGTCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCaa                                                                      <  1:106378/149‑1 (MQ=255)
                                                                             tgGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCaa                                                                      <  1:21418/149‑1 (MQ=255)
                                                                             tgGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCaa                                                                      >  1:293233/1‑149 (MQ=255)
                                                                                     tcatcGAGCAACGTCATTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAGCGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATAtct                                                              <  4:291831/149‑1 (MQ=255)
                                                                                     tcatcGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAGCGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATAtct                                                              <  4:291826/149‑1 (MQ=255)
                                                                                     tcatcGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACTGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATAtct                                                              >  7:200673/1‑149 (MQ=255)
                                                                                        tccaGCAACGTCAGTAAACAACTCCCCACCAAAAGCATTCCCTCACATATTTATTGATGAAGGGTGTATGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATAtctctc                                                           >  6:271800/4‑149 (MQ=255)
                                                                                        tcGAGCAACGTCAGTAAACTACTCCCTGCCAAAAGAATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGAAGCGCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATAtctctc                                                           <  5:35761/149‑1 (MQ=255)
                                                                                          gAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGc                                                         <  2:293233/149‑1 (MQ=255)
                                                                                           aGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGc                                                         >  7:249990/1‑148 (MQ=255)
                                                                                               aCGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCcagca                                                    <  3:40602/149‑1 (MQ=255)
                                                                                                     gTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGa                                              <  8:85155/149‑1 (MQ=255)
                                                                                                           tactCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCa                                        >  1:42982/1‑149 (MQ=255)
                                                                                                             ctCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCAc                                       <  5:382751/148‑1 (MQ=255)
                                                                                                               cccGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGtt                                    >  3:333305/1‑149 (MQ=255)
                                                                                                                ccGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGtt                                    >  3:82190/1‑148 (MQ=255)
                                                                                                                 cGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGtt                                    >  7:127207/1‑147 (MQ=255)
                                                                                                                 cGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGtt                                    <  8:127207/147‑1 (MQ=255)
                                                                                                                      aaaaGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTAt                              >  6:38394/1‑148 (MQ=255)
                                                                                                                        aaGCTTTCCCTCTCATTTTTATTGTTGAAGTGTGTAAGTATGAAGCAAATACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGaa                           <  2:379720/149‑1 (MQ=255)
                                                                                                                                ccTCTCATTTTTATTGTTGGAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCttt                   <  8:174391/149‑1 (MQ=255)
                                                                                                                                ccTCTCATTTTTATTGTTGGAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCttt                   <  8:179956/149‑1 (MQ=255)
                                                                                                                                ccTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCttt                   >  5:102068/1‑149 (MQ=255)
                                                                                                                                ccTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTATACTATGAACCACCttt                   >  8:291133/1‑149 (MQ=255)
                                                                                                                                ccTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGATTCACAGGTCAACATTTTTTAAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCttt                   >  1:129349/1‑149 (MQ=255)
                                                                                                                                    tcATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCaa                                                                            <  4:35669/88‑1 (MQ=255)
                                                                                                                                    tcATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCttttt                 <  2:42982/147‑1 (MQ=255)
                                                                                                                                      aTTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTa                <  5:38394/146‑1 (MQ=255)
                                                                                                                                          ttATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGTCAGCTCTGATACTTGCTTCACATGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCCGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCa          <  2:268437/148‑1 (MQ=255)
                                                                                                                                          ttATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCAc         >  7:28294/1‑149 (MQ=255)
                                                                                                                                             ttgttgAATGCTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCCGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGt      <  8:125459/149‑1 (MQ=255)
                                                                                                                                                ttgAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGa    <  4:82190/148‑1 (MQ=255)
                                                                                                                                                ttgAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGa    <  8:200673/148‑1 (MQ=255)
                                                                                                                                                  gAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGAgg  >  8:201796/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGAGG  >  NC_000913/2029241‑2029534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑