Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,448,244 G→A 5.5% A66V (GCG→GTG)  prmB ← N5‑glutamine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,448,2440GA 5.5% 230.1 / 2.4 72A66V (GCG→GTG) prmBN5‑glutamine methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (31/37);  new base A (2/2);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

CGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCC  >  NC_000913/2448102‑2448390
                                                                                                                                              |                                                                                                                                                  
cgGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTgc                                                                                                                                              <  4:233887/149‑1 (MQ=255)
   cACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCa                                                                                                                                           <  8:252149/149‑1 (MQ=255)
   cACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCa                                                                                                                                           <  4:118186/149‑1 (MQ=255)
    aCCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCat                                                                                                                                          <  3:163266/149‑1 (MQ=255)
       aGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATc                                                                                                                                       >  3:93735/1‑149 (MQ=255)
        gCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCt                                                                                                                                      <  8:179551/149‑1 (MQ=255)
                 cATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAata                                                                                                                             >  8:202407/1‑149 (MQ=255)
                  aTCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAatat                                                                                                                            >  6:384820/1‑149 (MQ=255)
                   tCGACGTAAAATTCATGGCCGCAGACCCCCGCTTTGTTGGTCAGGTAAGCCACCGGAGTGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTACGGAATATc                                                                                                                           >  1:147984/1‑149 (MQ=255)
                     gatgTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATcc                                                                                                                          <  8:266488/145‑1 (MQ=255)
                      aCGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAg                                                                                                                        >  6:360618/1‑149 (MQ=255)
                      aCGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAg                                                                                                                        <  5:206717/149‑1 (MQ=255)
                          aaaaTTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCgg                                                                                                                     >  3:170273/1‑148 (MQ=255)
                          aaaaTTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCgg                                                                                                                     >  7:388258/1‑148 (MQ=255)
                           aaaTTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGc                                                                                                                    <  4:93735/148‑1 (MQ=255)
                           aaaTTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGc                                                                                                                    <  7:202407/148‑1 (MQ=255)
                                           aaCCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCaa                                                                                                   >  2:231024/1‑149 (MQ=255)
                                           aaCCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCaa                                                                                                   <  4:170273/149‑1 (MQ=255)
                                              cACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAacac                                                                                                >  2:79516/1‑149 (MQ=255)
                                               aCGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACAcc                                                                                               <  3:313384/149‑1 (MQ=255)
                                                  cTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGc                                                                                            <  5:360618/149‑1 (MQ=255)
                                                   tttGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTACGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCt                                                                                           >  5:322612/1‑149 (MQ=255)
                                                      gttgGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGt                                                                                         <  5:384820/148‑1 (MQ=255)
                                                          gTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCTGAATATCAAGCGGCAGTTAGAGCGAAGGACACACCAGCTGTACGGc                                                                                    >  2:132068/1‑149 (MQ=255)
                                                          gTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAAAACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGc                                                                                    >  5:4180/1‑149 (MQ=255)
                                                           tCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCt                                                                                   <  8:180937/149‑1 (MQ=255)
                                                           tCAGGTAAGCCACCGGAATGCGGTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCt                                                                                   <  6:223324/149‑1 (MQ=255)
                                                              ggTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGGGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCa                                                                                >  8:226243/1‑149 (MQ=255)
                                                               gTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGaa                                                                                                        >  1:172710/1‑124 (MQ=255)
                                                               gTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGaa                                                                                                        <  2:172710/124‑1 (MQ=255)
                                                                    ccACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCAc                                                                          <  6:321785/149‑1 (MQ=255)
                                                                     cACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACCCCAGCTGTACGGCTTCATCCCACg                                                                         >  1:333747/1‑149 (MQ=255)
                                                                      acgtgAATGCGTTCATTGACGCGGCGGAGCAGGCGTTCAACAATACAGTGTTTTTCGCTGGAGGCCAGACGCGCGGTGCGCATATCTTCCGGAATATGCTGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACgg                                                                        <  1:240611/145‑1 (MQ=255)
                                                                      aCCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACgg                                                                        <  8:388258/149‑1 (MQ=255)
                                                                       ccGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACggg                                                                       <  5:44849/149‑1 (MQ=255)
                                                                        cGGACTGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGTTCAGACGCGCGTTGCGCATATCTTCCGGAATATCCAGCGTCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGt                                                                      <  8:106890/149‑1 (MQ=255)
                                                                        cGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGCGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCACCACCAGCTGGACGGCTTCATCCCACGGGt                                                                      >  6:151909/1‑149 (MQ=255)
                                                                           aaTGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTAt                                                                   <  4:210522/149‑1 (MQ=255)
                                                                           aaTGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTAt                                                                   <  1:79516/149‑1 (MQ=255)
                                                                              gCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCgg                                                                >  5:268059/1‑149 (MQ=255)
                                                                                gTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATc                                                                  <  4:366012/145‑1 (MQ=255)
                                                                                gTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATc                                                                  >  3:366012/1‑145 (MQ=255)
                                                                                gTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTa                                                              >  6:95448/1‑149 (MQ=255)
                                                                                    aTTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAg                                                                                                                  <  6:327971/93‑1 (MQ=255)
                                                                                    aTTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAg                                                                                                                  >  5:327971/1‑93 (MQ=255)
                                                                                        aCGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGAcc                                                      >  6:92933/1‑149 (MQ=255)
                                                                                        aCGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGAc                                                       <  8:102039/148‑1 (MQ=255)
                                                                                          gcggcgGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCACGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg                                                     >  3:88663/1‑148 (MQ=255)
                                                                                          gcggcgGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCACGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg                                                     >  3:83522/1‑148 (MQ=255)
                                                                                            ggcggATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCATCCGGAATATCCAGCGGAAGGTAGAGCGAAGGCAACACCATCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTAc                                                  <  4:320150/149‑1 (MQ=255)
                                                                                                 aTCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGat                                             <  1:231024/149‑1 (MQ=255)
                                                                                                  tCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGata                                            >  7:269329/1‑149 (MQ=255)
                                                                                                    aCGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGtt                                                                     <  4:70966/122‑1 (MQ=255)
                                                                                                    aCGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGtt                                                                     >  3:70966/1‑122 (MQ=255)
                                                                                                      gcgTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTg                                        <  7:120124/149‑1 (MQ=255)
                                                                                                        gTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGcc                                      <  6:268059/149‑1 (MQ=255)
                                                                                                          tCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCcgc                                    >  8:264079/1‑149 (MQ=255)
                                                                                                           caacaaTACGGTGTTTTTCGCTGGAGGTCAGACGCACGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCcgc                                    <  4:83522/148‑1 (MQ=255)
                                                                                                           caacaaTACGGTGTTTTTCGCTGGAGGTCAGACGCACGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCcgc                                    <  4:88663/148‑1 (MQ=255)
                                                                                                            aacaaTACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCGGa                                              >  8:407517/1‑137 (MQ=255)
                                                                                                            aacaaTACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCGGa                                              <  7:407517/137‑1 (MQ=255)
                                                                                                            aacaaTACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGa                                              >  8:407518/1‑137 (MQ=255)
                                                                                                            aacaaTACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGa                                              <  7:407518/137‑1 (MQ=255)
                                                                                                                  aCGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGc                                                                                                                    <  2:392693/61‑1 (MQ=255)
                                                                                                                  aCGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGc                                                                                                                    >  1:392693/1‑61 (MQ=255)
                                                                                                                        tttttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCa                      >  7:99703/1‑149 (MQ=255)
                                                                                                                          tttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCAcc                    >  7:101353/1‑149 (MQ=255)
                                                                                                                                tGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTTGAGCGAAGGCAACACCAGCTGTCCGGCTTCCTCCCACGGGTTATCGGTACCGGGCCCGTTCCAGCTTTTTGCCGCGCGGCAGCGGCTCAGCgcgccg              >  4:293446/1‑144 (MQ=255)
                                                                                                                                     gTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGTCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCaa         <  7:226243/149‑1 (MQ=255)
                                                                                                                                          aCGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGTTAGAGCGAAGGCAACACCTGCTGTACGGCTTCATCCCAAGGGTTATCGGTACCGTGACCGTACCAGATATTTTCCGCGCTGAAGCGGCTCACCTACCAGCGCAACATg     <  4:268952/148‑1 (MQ=255)
                                                                                                                                          aCGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGt    <  6:4180/149‑1 (MQ=255)
                                                                                                                                          aCGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGt    >  5:221968/1‑149 (MQ=255)
                                                                                                                                           cgcgcgGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTc   <  7:264079/149‑1 (MQ=255)
                                                                                                                                            gcgcgGGGCGCATATCTTCCGGAATATCCAGCGGCATGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTcc  <  1:315733/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCC  >  NC_000913/2448102‑2448390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑