Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,310,417	IS2 (–) +5 bp	coding (560‑564/1533 nt)	alsA ←	D‑allose ABC transporter ATPase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	70 (1.910)	54/242	0.0	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	4310417 =	0 (0.000)					coding (564/1533 nt)	alsA	D‑allose ABC transporter ATPase
*	?	NC_000913	= 4310421	0 (0.000)	42 (1.150)	34/240	0.2	100%	coding (560/1533 nt)	alsA	D‑allose ABC transporter ATPase
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

CGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGAt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4310275‑4310421 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCT  <  NC_000913/4499511‑4499367                                                                                                                                                                                                                                                                                                      CGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCTCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCTACTGATTCATGAT                                                                                                                                                  <  8:160582/148‑1         GACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGC                                                                                                                                          <  6:191861/148‑1                                  AAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTAT                                                                                                                 >  1:116169/1‑148                                                      ATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAA                                                                                                <  1:85351/145‑1                                                      ATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAA                                                                                                >  2:85351/1‑145                                                      ATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTT                                                                                                            >  8:49005/1‑133                                                      ATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTT                                                                                                            <  7:49005/133‑1                                                       TCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCG                                                                                           >  2:90862/1‑149                                                               TAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGC                                                                                    <  2:116169/148‑1                                                                         AAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAA                                                                                                <  8:55794/126‑1                                                                         AAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAA                                                                                                >  7:55794/1‑126                                                                                          GCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGGCATCTGTTATCAC                                                                                                              >  3:102057/1‑95                                                                                          GCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGGCATCTGTTATCAC                                                                                                              <  4:102057/95‑1                                                                                              ACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTA                                                                                                   <  6:93279/102‑1                                                                                              ACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTA                                                                                                   >  5:93279/1‑102                                                                                                TTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCC                                                                                                       >  3:56277/1‑96                                                                                                TTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCC                                                                                                       <  4:56277/96‑1                                                                                                          TATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTT                                                                                                                                            >  6:65940/1‑49                                                                                                          TATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTT                                                                                                                                            <  5:65940/49‑1                                                                                                             AGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGT                                                                        >  1:76265/1‑114                                                                                                             AGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGT                                                                        <  2:76265/114‑1                                                                                                              GACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAT                                    >  3:27516/1‑149                                                                                                                       CGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGG                                                                      >  6:38855/1‑106                                                                                                                       CGTACCCTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGG                                                                      <  5:38855/106‑1                                                                                                                             CTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGAT                                                                                <  6:35255/90‑1                                                                                                                             CTCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGAT                                                                                >  5:35255/1‑90                                                                                                                              TCTTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCG                     >  2:68917/1‑148                                                                                                                              TCTTTACGCAACTGATTCATGATGGATTTGCCCCTA                                                                                                                                     <  2:115061/36‑1                                                                                                                              TCTTTACGCAACTGATTCATGATGGATTTGCCCCTA                                                                                                                                     <  2:117955/36‑1                                                                                                                              TCTTTACGCAACTGATTCATGATGGATTTGCCCCTA                                                                                                                                     >  1:117955/1‑36                                                                                                                              TCTTTACGCAACTGATTCATGATGGATTTGCCCCTA                                                                                                                                     >  1:115061/1‑36                                                                                                                                TTTACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCG                     <  1:68917/146‑1                                                                                                                                   ACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTG                                                                       <  2:8395/93‑1                                                                                                                                   ACGCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTG                                                                       >  1:8395/1‑93                                                                                                                                     GCAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTC             >  3:88023/1‑149                                                                                                                                      CAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGAT                                                                                <  2:95804/81‑1                                                                                                                                      CAACTGATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGAT                                                                                >  1:95804/1‑81                                                                                                                                            ATTCATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAG       <  1:90862/148‑1                                                                                                                                                ATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCT  >  5:3663/1‑149                                                                                                                                                ATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGC                                             >  4:22189/1‑106                                                                                                                                                ATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGC                                             <  3:22189/106‑1                                                                                                                                                ATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCC                                                         <  4:18427/94‑1                                                                                                                                                ATGATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCC                                                         >  3:18427/1‑94                                                                                                                                                                                                                                                                                                      CGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGAt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4310275‑4310421 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCT  <  NC_000913/4499511‑4499367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.