| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,706,079 | 1 | . | T | 23.5% | 30.4 / 14.3 | 17 | coding (313/582 nt) | rsxA | SoxR iron‑sulfur cluster reduction factor component; inner membrane protein of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base . (7/6); new base T (2/2); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGGATTTTGATCCCACTTAATCTGATTTACCTGCGCACCCTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑TTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGT > NC_000913/1705949‑1706206 | gTGGATTTTGATCCCACTTAATCTGATTTACCTGCGCACCCTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑TTTTTTTGCCGCTTATca > 4:83821/1‑149 (MQ=255) tCCCACTTAATCTGATTTACCTGCGCACCCTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑TTTTTTTGCCGCTTATCACCACCAACtgt > 7:146487/1‑149 (MQ=255) cccACTTAATCTGATTTACCTGCGCACCCTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTTGCCGCTTATCACCACCAACtgt > 2:63921/1‑149 (MQ=255) cccACTTAATCTGATTTACCTGCGCACCCTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTTGCCGCTTATCACCACCAACtgt > 2:66761/1‑149 (MQ=255) cACTTAATCTGATTTACCTGCGCACCCTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑ttttttt < 6:143626/124‑1 (MQ=255) cACTTAATCTGATTTACCTGCGCACCCTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑ttttttt > 5:143626/1‑124 (MQ=255) aCTTAATCTGATTTACCTGCGCACCCTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTTGCCGCTTATCACCACCAACTGTGCa < 1:63921/149‑1 (MQ=255) aCTTAATCTGATTTACCTGCGCACCCTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTTGCCGCTTATCACCACCAACTGTGCa < 1:66761/149‑1 (MQ=255) aTTTACCTGCGCACCCTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑TTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCggcgt < 3:83821/149‑1 (MQ=255) ccTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑TTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAata < 8:146487/149‑1 (MQ=255) cTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑TTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAatat > 1:152073/1‑149 (MQ=255) aCCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑TTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACgg < 2:152073/149‑1 (MQ=255) ccGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑TTTTTTTGCCGCTTATCACCACCAACTGTGCa < 7:133261/83‑1 (MQ=255) ccGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑TTTTTTTGCCGCTTATCACCACCAACTGTGCa > 8:133261/1‑83 (MQ=255) cgcAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑TTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAat < 5:76999/93‑1 (MQ=255) cgcAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑TTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAat > 6:76999/1‑93 (MQ=255) gTGCTTTACCGTCTGCTGGGGA‑TTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGt > 5:189678/1‑149 (MQ=255) gctgGGGA‑TTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGtt > 2:50393/1‑107 (MQ=255) gctgGGGA‑TTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGtt < 1:50393/107‑1 (MQ=255) | GTGGATTTTGATCCCACTTAATCTGATTTACCTGCGCACCCTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGA‑TTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGT > NC_000913/1705949‑1706206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |