Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	457,671	A→G	E82E (GAA→GAG)	clpX →	ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	457,671	0	A	G	100.0%	75.2 / NA	22	E82E (GAA→GAG)	clpX	ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/13);  total (9/13)

TATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTC  >  NC_000913/457528‑457783                                                                                                                                                |                                                                                                                 tataTCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAgg                                                                                                              <  5:21882/148‑1 (MQ=255)             gaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCt                                                                                                 >  2:28325/1‑149 (MQ=255)                       aTTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGt                                                                                                    <  3:121419/136‑1 (MQ=255)                       aTTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGt                                                                                                    >  4:121419/1‑136 (MQ=255)                       aTTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTcgcg                                                                                       <  7:164617/149‑1 (MQ=255)                          tATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTTCGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGt                                                                                     <  8:45236/148‑1 (MQ=255)                                       catTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTAc                                                                        >  6:79735/1‑148 (MQ=255)                                        atTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACa                                                                       >  2:197715/1‑148 (MQ=255)                                             cgAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTgg                                                                                               <  3:150364/119‑1 (MQ=255)                                             cgAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTgg                                                                                               >  4:150364/1‑119 (MQ=255)                                              gAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCt                                                                <  1:28325/149‑1 (MQ=255)                                                    aTTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCaa                                                          >  8:115363/1‑149 (MQ=255)                                                             gTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATAc                                                 <  5:79735/149‑1 (MQ=255)                                                                gCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCa                                               <  1:115163/148‑1 (MQ=255)                                                                   ccGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCaa                                           <  1:197715/149‑1 (MQ=255)                                                                       aTCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGaaaaaa                                                                                                      <  1:119689/86‑1 (MQ=255)                                                                       aTCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGaaaaaa                                                                                                      >  2:119689/1‑86 (MQ=255)                                                                               cGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGtt                               <  7:115363/149‑1 (MQ=255)                                                                                         tACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAg                                              <  7:103833/124‑1 (MQ=255)                                                                                         tACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAg                                              >  8:103833/1‑124 (MQ=255)                                                                                                      tGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGa        >  4:131863/1‑149 (MQ=255)                                                                                                             cGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTc  <  3:131863/148‑1 (MQ=255)                                                                                                                                                |                                                                                                                 TATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTC  >  NC_000913/457528‑457783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑