Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,020,511 C→G 5.0% intergenic (+22/‑29) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,020,5110CG 5.0% 317.7 / 7.7 100intergenic (+22/‑29)ssnA/ygfMputative chlorohydrolase/aminohydrolase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (52/42);  new base G (2/3);  total (54/46)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.84e-01

CGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCC  >  NC_000913/3020366‑3020657
                                                                                                                                                 |                                                                                                                                                  
cGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAgg                                                                                                                                                 >  1:191776/1‑149 (MQ=255)
 gTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAggg                                                                                                                                                <  2:161739/149‑1 (MQ=255)
  tGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAggg                                                                                                                                                >  2:200445/1‑148 (MQ=255)
     tGGTCAATGGTGTGATGCTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAaga                                                                                                                                            <  3:247998/149‑1 (MQ=255)
       gTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCAATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAAGAgg                                                                                                                                          <  6:368722/149‑1 (MQ=255)
         cAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCGCTCTTCCCGCAGGGAAGAGGGt                                                                                                                                        >  7:301344/1‑149 (MQ=255)
          aaTGGTGTGATGGTCTATGAAGACCGTCAGTTTACCTTCGATTGCGATTCCATTTATTCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGTATGCGCTGGCAGAAATGCCAGATGCCCTCTTCCAGCATGGAATAGGGt                                                                                                                                        >  7:270020/1‑148 (MQ=255)
             ggTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATGCCGATTCCATTTCTGCACCAGCCAGAACAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCGCTTCCCGCAGGGAAGAGGGTTaaa                                                                                                                                    <  5:356271/149‑1 (MQ=255)
                 tgATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAg                                                                                                                                <  8:305052/149‑1 (MQ=255)
                 tgATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAg                                                                                                                                >  8:285243/1‑149 (MQ=255)
                   attgTCTATGAAGACCGTCAGTTTATCTTCGATTGCGATTCCATTTATGCACAATCCAGAAAAGCCGCTGCCAGTTTGTGGCGTCGGTTGGATGCGCTGGCATAAATGAAAGATGCCCTCTTACCGCAGGGAAGAGGGTTAAATAAGGa                                                                                                                              <  2:150370/146‑1 (MQ=255)
                    tGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGaa                                                                                                                             <  8:383981/149‑1 (MQ=255)
                    tGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGa                                                                                                                              >  8:391421/1‑148 (MQ=255)
                    tGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTACCGCACGGAAGAGGATTAAATAAGGa                                                                                                                              >  4:250983/1‑148 (MQ=255)
                      gTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGa                                                                                                                              >  4:167378/1‑146 (MQ=255)
                         tATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAaag                                                                                                                           >  3:357651/1‑146 (MQ=255)
                              aGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAAGAgg                                                                                                                                          <  6:203797/126‑1 (MQ=255)
                              aGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAAGAgg                                                                                                                                          >  5:203797/1‑126 (MQ=255)
                              aGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatgat                                                                                                                   >  4:226948/1‑149 (MQ=255)
                               gACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt                                                                                                                  <  4:363874/149‑1 (MQ=255)
                               gACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt                                                                                                                  >  2:322107/1‑149 (MQ=255)
                                aCCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTg                                                                                                                 <  7:391421/149‑1 (MQ=255)
                                 ccGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGa                                                                                                                <  1:322107/149‑1 (MQ=255)
                                  cGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGaa                                                                                                               >  5:225569/1‑149 (MQ=255)
                                  cGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGaa                                                                                                               >  1:193121/1‑149 (MQ=255)
                                    tCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCCATGGCATAAATGACAGATCCCCTATTCCAGCAGGGAACAGGGTTAAATAAGGAAAGAAGAACATTCAAc                                                                                                              >  8:56542/1‑148 (MQ=255)
                                        tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAttt                                                                                                         >  1:362077/1‑149 (MQ=255)
                                        tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAttt                                                                                                         >  7:95493/1‑149 (MQ=255)
                                            aCTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAttttt                                                                                                       >  8:125702/1‑147 (MQ=255)
                                            aCTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                     <  8:95493/149‑1 (MQ=255)
                                            aCTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGATGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                     <  1:173898/149‑1 (MQ=255)
                                              ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGa                                                                                                                              <  8:375776/122‑1 (MQ=255)
                                              ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGa                                                                                                                              >  7:375776/1‑122 (MQ=255)
                                              ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgat                                                                                                                      >  7:390899/1‑130 (MQ=255)
                                              ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgat                                                                                                                      <  8:390899/130‑1 (MQ=255)
                                                cGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGcc                                                                                                 >  4:225007/1‑149 (MQ=255)
                                                      cGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGActc                                                                                           >  4:206460/1‑149 (MQ=255)
                                                       gATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                     <  6:277162/138‑1 (MQ=255)
                                                       gATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                     >  5:277162/1‑138 (MQ=255)
                                                       gATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGCTTAAATAAGGAAAGAAGATGAGTGAACAATTTTTCAGGCCCGActct                                                                                          >  6:306285/1‑149 (MQ=255)
                                                           ccATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTc                                                                                       <  2:191776/148‑1 (MQ=255)
                                                             aTTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGaa                                                                                    >  8:268835/1‑149 (MQ=255)
                                                             aTTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGaa                                                                                    >  7:131466/1‑149 (MQ=255)
                                                             aTTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGaa                                                                                    <  1:200445/149‑1 (MQ=255)
                                                                     acaAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCt                                                                            >  2:177958/1‑149 (MQ=255)
                                                                        aGCCCGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGa                                                                         <  7:56542/149‑1 (MQ=255)
                                                                          ccAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAAc                                                                       <  8:131466/149‑1 (MQ=255)
                                                                          ccAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAAc                                                                       >  8:340593/1‑149 (MQ=255)
                                                                          ccAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAAc                                                                       >  7:94730/1‑149 (MQ=255)
                                                                          ccAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAAc                                                                       <  4:357651/149‑1 (MQ=255)
                                                                            aGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACt                                                                      >  4:242125/1‑148 (MQ=255)
                                                                             gAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGATCAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTg                                                                     >  5:156355/1‑148 (MQ=255)
                                                                               ccaGCCTCTGCCATTATGTTGCGTCGTATGTATGCGATTGAATTTATGACTGATGCCGGCTTCCCGCAGGGAAGAGGGTTAGATAAGGAACGAATATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGTCGCTGGAACTGaa                                                                   <  8:270020/146‑1 (MQ=255)
                                                                                  gCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAgcgc                                                               >  2:297556/1‑149 (MQ=255)
                                                                                   ccGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAAc                                                                                   >  5:98721/1‑128 (MQ=255)
                                                                                   ccGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAAc                                                                                   <  6:98721/128‑1 (MQ=255)
                                                                                          cAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCa                                                       <  2:362077/149‑1 (MQ=255)
                                                                                           aGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAATTAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGCAGCGCCGCTACCAg                                                      >  1:193734/1‑149 (MQ=255)
                                                                                           aGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAg                                                      >  1:260453/1‑149 (MQ=255)
                                                                                            gTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAg                                                      >  3:65684/1‑148 (MQ=255)
                                                                                                gtgGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGaa                                                                                    <  7:337337/114‑1 (MQ=255)
                                                                                                gtgGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGaa                                                                                    >  8:337337/1‑114 (MQ=255)
                                                                                                 tgGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGaa                                                <  3:226948/149‑1 (MQ=255)
                                                                                                  ggCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAg                                               >  4:137581/1‑149 (MQ=255)
                                                                                                   gCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGc                                              >  4:275822/1‑149 (MQ=255)
                                                                                                     gTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCg                                            >  2:228200/1‑149 (MQ=255)
                                                                                                      tCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGt                                           <  8:94730/149‑1 (MQ=255)
                                                                                                       cGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTc                                          >  7:25226/1‑149 (MQ=255)
                                                                                                         gatggatgCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGccc                                                                                                >  1:319705/1‑93 (MQ=255)
                                                                                                         gatggatgCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGccc                                                                                                <  2:319705/93‑1 (MQ=255)
                                                                                                         gatggatgCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTg                                        <  8:25226/149‑1 (MQ=255)
                                                                                                          atggatgCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTgg                                       <  2:193121/149‑1 (MQ=255)
                                                                                                            ggatgCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGt                                      <  6:225569/148‑1 (MQ=255)
                                                                                                             gatgGGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTc                                    <  5:309984/149‑1 (MQ=255)
                                                                                                             gatgCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGtt                                     <  1:177958/148‑1 (MQ=255)
                                                                                                                  gcTGGCATAAATGACAGATGCCCTCTTCCCGAAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCgg                               <  2:260453/149‑1 (MQ=255)
                                                                                                                   cTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCggg                              <  7:285243/149‑1 (MQ=255)
                                                                                                                      gCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCggggg                            <  4:144601/148‑1 (MQ=255)
                                                                                                                        aTAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgcag                         >  7:264337/1‑149 (MQ=255)
                                                                                                                         tAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCAGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgcag                         >  5:314231/1‑148 (MQ=255)
                                                                                                                          aaaTGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAATGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgaagc                        >  6:369088/1‑148 (MQ=255)
                                                                                                                            aTGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGATGATTATTGAACAATTTTTCAGGCACGACTCTGTCGAACAGTCTCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTAGCCGGGGGCAGCaa                      >  3:146376/1‑148 (MQ=255)
                                                                                                                            aTGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCaaa                     <  3:242125/149‑1 (MQ=255)
                                                                                                                             tGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAAc                    >  3:246923/1‑149 (MQ=255)
                                                                                                                                 aGATGCCCTCTTCCCGCAGGTAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCaa                <  4:246923/149‑1 (MQ=255)
                                                                                                                                 aGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCaa                <  3:167378/149‑1 (MQ=255)
                                                                                                                                 aGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCaa                <  3:250983/149‑1 (MQ=255)
                                                                                                                                 aGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCaa                >  7:108242/1‑149 (MQ=255)
                                                                                                                                    tGCGCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGTGCAGCAAACTCAATGc             <  8:301344/149‑1 (MQ=255)
                                                                                                                                    tGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGc             <  3:225007/149‑1 (MQ=255)
                                                                                                                                      cccTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTa           >  3:89691/1‑149 (MQ=255)
                                                                                                                                       ccTCTTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAGCAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTa           >  1:31140/1‑148 (MQ=255)
                                                                                                                                       ccTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTa           >  1:24648/1‑148 (MQ=255)
                                                                                                                                         tctTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTacac        >  3:211192/1‑149 (MQ=255)
                                                                                                                                              ccGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTg                                                                           >  6:103608/1‑77 (MQ=255)
                                                                                                                                              ccGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTg                                                                           <  5:103608/77‑1 (MQ=255)
                                                                                                                                              ccGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAAc    <  1:228200/148‑1 (MQ=255)
                                                                                                                                               cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCt                                         <  6:49423/110‑1 (MQ=255)
                                                                                                                                               cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAAccc  <  8:108242/149‑1 (MQ=255)
                                                                                                                                               cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGCCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAAccc  >  8:177677/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCC  >  NC_000913/3020366‑3020657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: