Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,618,826 A→C 7.3% D80A (GAC→GCC)  nikR → transcriptional repressor, Ni‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,618,8260AC 7.3% 205.9 / 2.6 79D80A (GAC→GCC) nikRtranscriptional repressor, Ni‑binding
Reads supporting (aligned to +/- strand):  ref base A (38/35);  new base C (4/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTG  >  NC_000913/3618678‑3618974
                                                                                                                                                    |                                                                                                                                                    
gCTATCCGCGACATTCTGCGTAGCGACCTGGCGCAAGAGGCCACCCAGCAGCAAGGCAAGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGa                                                                                                                                                      <  7:350109/149‑1 (MQ=255)
 cTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGAc                                                                                                                                                     <  7:339971/149‑1 (MQ=255)
      cgcgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCtctc                                                                                                                                                >  5:162119/1‑149 (MQ=255)
         gACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGt                                                                                                                                             <  6:273468/149‑1 (MQ=255)
               cTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCa                                                                                                                                        <  6:278689/148‑1 (MQ=255)
                    tAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTg                                                                                                                                   >  6:277147/1‑148 (MQ=255)
                      gcgcCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGAGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCAt                                                                                                                                <  8:94389/149‑1 (MQ=255)
                        gcCCTGGCGCAAGAGGCCCCCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCCGTGTCTCCACCCAGCATCATCTCCACGCCCTCTCCGTCGCCACGCTGCAggt                                                                                                                              >  5:13846/1‑149 (MQ=255)
                              gcgcAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGcaca                                                                                                                         <  2:172864/148‑1 (MQ=255)
                                gcAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATc                                                                                                                       >  2:249645/1‑148 (MQ=255)
                                 cAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGACCACGATAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGCCCTCTCCGTCGCCACGCTGCATGTGCACATCaa                                                                                                                     >  3:224934/1‑149 (MQ=255)
                                 cAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCaa                                                                                                                     >  7:175419/1‑149 (MQ=255)
                                  aaGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAAc                                                                                                                    <  6:162119/149‑1 (MQ=255)
                                   agagGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAAcc                                                                                                                   <  5:277147/149‑1 (MQ=255)
                                      ggCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCac                                                                                                                 >  3:277056/1‑148 (MQ=255)
                                       gCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCCGCCGCATTGTCTCCACCCAGCATCAGCACCACGCCCTCTCCGTCGCCACGCTGCATGTGCACATCAACCacga                                                                                                               >  7:239412/1‑149 (MQ=255)
                                       gCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCacga                                                                                                               <  8:87617/149‑1 (MQ=255)
                                          aCCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCAcgacga                                                                                                            <  1:319758/149‑1 (MQ=255)
                                          aCCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCAcgacga                                                                                                            >  2:319758/1‑149 (MQ=255)
                                          aCCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCAcgacga                                                                                                            >  7:24386/1‑149 (MQ=255)
                                           cccAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACgacgac                                                                                                           <  7:344030/149‑1 (MQ=255)
                                              agcagcACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCTCCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGt                                                                                                        <  8:151077/149‑1 (MQ=255)
                                               gcagcaCGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTc                                                                                                       >  3:342471/1‑149 (MQ=255)
                                               gcagcaCGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTc                                                                                                       <  4:342471/149‑1 (MQ=255)
                                                cagcaCGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCt                                                                                                      >  7:138580/1‑149 (MQ=255)
                                                 agcaCGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTg                                                                                                     >  3:41303/1‑149 (MQ=255)
                                                  gcacggcacgCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTgg                                                                                                    <  8:138580/149‑1 (MQ=255)
                                                   cacggcacgCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCAACGCTGCATGTGCACATCAACCACGACGACTGTCTGGa                                                                                                   <  4:45047/149‑1 (MQ=255)
                                                       gcacgCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATc                                                                                               >  5:336287/1‑149 (MQ=255)
                                                            cAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTg                                                                                                     >  6:239383/1‑138 (MQ=255)
                                                            cAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTg                                                                                                     <  5:239383/138‑1 (MQ=255)
                                                             aaGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCgtg                                                                                         >  5:118974/1‑149 (MQ=255)
                                                               ggTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGtt                                                                                       <  8:24386/149‑1 (MQ=255)
                                                                     gcgGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAgg                                                                                 <  7:237401/149‑1 (MQ=255)
                                                                     gcgGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAg                                                                                  <  6:336287/148‑1 (MQ=255)
                                                                          gCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACa                                                                            >  6:207138/1‑149 (MQ=255)
                                                                           cTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACAt                                                                           >  2:289829/1‑149 (MQ=255)
                                                                                gTATGTGTATGAACACGAAAAACGAGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGt                                                                       <  1:64319/148‑1 (MQ=255)
                                                                                   tgtgTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGAc                                                                    <  3:78193/148‑1 (MQ=255)
                                                                                    gtgtATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGt                                                                  >  4:244924/1‑149 (MQ=255)
                                                                                       tATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTgc                                                                <  1:101184/148‑1 (MQ=255)
                                                                                       tATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAAACACGACGACTGTCTGTAAAACGCCTTGTTGAAATGTGACATGGATGACGTg                                                                 >  6:337224/1‑147 (MQ=255)
                                                                                         tGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTgcag                                                              >  7:139042/1‑148 (MQ=255)
                                                                                          gAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGt                                                                       >  8:307234/1‑138 (MQ=255)
                                                                                          gAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGt                                                                       <  7:307234/138‑1 (MQ=255)
                                                                                            acacGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCAtt                                                          >  3:19534/1‑149 (MQ=255)
                                                                                            acacGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCAtt                                                          >  2:252286/1‑149 (MQ=255)
                                                                                            acacGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCAtt                                                          >  2:200836/1‑149 (MQ=255)
                                                                                             cacGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATGATCACCACGACCTCTGCGTCTCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGTGTGACGTGCAGCAttt                                                         <  6:291783/149‑1 (MQ=255)
                                                                                             cacGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGATATCGCCGGGTTGAAAGGTGACATGGGTGGCGTGCAGCAtgt                                                         >  8:363733/1‑147 (MQ=255)
                                                                                             cacGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCAttt                                                         >  2:145407/1‑149 (MQ=255)
                                                                                              acGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCAtttt                                                        <  1:249645/149‑1 (MQ=255)
                                                                                                        cgACTTCGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACg                                              <  4:224934/149‑1 (MQ=255)
                                                                                                         gACTTAGCCAGCCGCTTTGTCTCCACCCAGCATCAGCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGt                                             <  8:239412/149‑1 (MQ=255)
                                                                                                              agccagccGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCg                                        >  6:238023/1‑149 (MQ=255)
                                                                                                               gccagccGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCacga                                                                                                               >  1:22193/1‑77 (MQ=255)
                                                                                                               gccagccGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCacga                                                                                                               <  2:22193/77‑1 (MQ=255)
                                                                                                                         ttGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCgcggc                             <  1:289829/149‑1 (MQ=255)
                                                                                                                         ttGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCgcggc                             <  8:139042/149‑1 (MQ=255)
                                                                                                                             ctcCACCCAGCATCATCACCACGCCCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGc                         >  5:291860/1‑149 (MQ=255)
                                                                                                                               ccACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGc                                                                                                                                  <  2:187431/42‑1 (MQ=255)
                                                                                                                               ccACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGc                                                                                                                                  >  1:187431/1‑42 (MQ=255)
                                                                                                                               ccACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCgg                       >  5:22937/1‑149 (MQ=255)
                                                                                                                                ccccccGCAGCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACCTCAACCACGACGTCTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGGGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGc                         <  6:118974/144‑1 (MQ=255)
                                                                                                                                cACCCAGCATCATCACCACGCCCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGc                      <  6:291860/149‑1 (MQ=255)
                                                                                                                                cACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCACCCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGCTGACGTTATCGCCCAGCGCGGCGTGCGGc                      >  2:249562/1‑149 (MQ=255)
                                                                                                                                cACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCgg                       <  6:22937/148‑1 (MQ=255)
                                                                                                                                 aCCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCa                     >  1:220842/1‑149 (MQ=255)
                                                                                                                                 aCCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCa                     >  8:250124/1‑149 (MQ=255)
                                                                                                                                 aCCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCa                     >  3:216810/1‑149 (MQ=255)
                                                                                                                                   ccAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACg                   >  8:224375/1‑149 (MQ=255)
                                                                                                                                    cAGCATCCTCCCCCCGCCCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGCCTGGAAATCGCCCTGTTGAAAGGTGACATGGGTGACGCGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACgg                  <  8:175419/149‑1 (MQ=255)
                                                                                                                                    cAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCACCCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGGACgg                  >  1:343629/1‑149 (MQ=255)
                                                                                                                                          catcaCCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCAttt            >  2:176869/1‑149 (MQ=255)
                                                                                                                                              cccacGACCTCTCCTTCGCCACGCTGCATGTGCACATCAACCCCGACGACTGTCTGGAAATCGCCGTGGTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCa         <  8:136983/147‑1 (MQ=255)
                                                                                                                                              accagGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCCCGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGATATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCa         <  3:93354/148‑1 (MQ=255)
                                                                                                                                              accacGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAg        <  4:41303/149‑1 (MQ=255)
                                                                                                                                               ccacGACCTCTCCGTCGCCTCGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGt       >  2:88129/1‑149 (MQ=255)
                                                                                                                                                cacGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGCTGACGTTATCGCCCAGCGCGGCGTGCGGCCCGGGCATTTGCAGTg      >  6:97830/1‑149 (MQ=255)
                                                                                                                                                 acGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGc     >  5:58316/1‑149 (MQ=255)
                                                                                                                                                    aCCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTg  <  3:244924/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTG  >  NC_000913/3618678‑3618974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: