Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,262,561 C→T 5.7% G106D (GGT→GAT)  ispE ← 4‑diphosphocytidyl‑2‑C‑methylerythritol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,262,5610CT 5.7% 226.9 / 2.7 71G106D (GGT→GAT) ispE4‑diphosphocytidyl‑2‑C‑methylerythritol kinase
Reads supporting (aligned to +/- strand):  ref base C (32/34);  new base T (2/2);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.33e-01

CTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTC  >  NC_000913/1262415‑1262706
                                                                                                                                                  |                                                                                                                                                 
cTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGGCCGCCCCCTTTCCGCCCGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGAGTTAATGCCACCAGGACCGTCGCGGCATTGGATTAACCAccg                                                                                                                                                 >  4:242/1‑149 (MQ=255)
   cGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCCGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCAccgccg                                                                                                                                              >  2:205502/1‑149 (MQ=255)
     gCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCgag                                                                                                                                            <  3:110673/149‑1 (MQ=255)
      cAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCgaga                                                                                                                                           <  1:76231/149‑1 (MQ=255)
       aaaCGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGAc                                                                                                                                          >  8:129694/1‑149 (MQ=255)
              gCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCgccgcc                                                                                                                                   >  3:320618/1‑149 (MQ=255)
                 tGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGCCCGTCGCGGCATTGGATGAACCCCCGCCTAGACCGCCGGCCAt                                                                                                                                >  6:285265/1‑149 (MQ=255)
                   cccccGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGGTGAACCACCGCCGAGACCGCCGCCCATCg                                                                                                                              <  8:54641/149‑1 (MQ=255)
                   cccccGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATc                                                                                                                               <  6:127077/148‑1 (MQ=255)
                   cccccGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATc                                                                                                                               <  7:264651/148‑1 (MQ=255)
                   cccccGAACAAAGACAGGAAAATCTGCGCCCAGCGTCATCCCCATTTCCCCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATc                                                                                                                               <  4:11059/148‑1 (MQ=255)
                    ccccGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCgg                                                                                                                             >  8:22287/1‑149 (MQ=255)
                     cccGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCgg                                                                                                                             >  4:126155/1‑148 (MQ=255)
                     cccGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGc                                                                                                                            <  5:203392/149‑1 (MQ=255)
                      ccGAACAAAGACAGGAACATCTGCGCCCAGCGTGAGCCCCATTTCCGCCAGCTCATCCATTCTTAACCCGCATTGCCAGAGATGATTTAATGCCACCCGGACCGTCGCGGCATTCGATGAACCACAGCCGACACCCCCGCCAATCGGc                                                                                                                            >  2:339066/1‑148 (MQ=255)
                      ccGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGc                                                                                                                            >  8:56892/1‑148 (MQ=255)
                      ccGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGc                                                                                                                            >  2:77111/1‑148 (MQ=255)
                             aaGTCAGGAAGATCTGCGCGCTGCGTCATCCCCATTTCCGCCATCTCATCCATGGTTAGCCAGCATTGCCAGATATGATTTAATGCCACCAGGACCGTCGCGGGATTGGATGAACCACCGAAGAGACCGCCGCCAATCGGCAAACGCtt                                                                                                                    <  4:320618/149‑1 (MQ=255)
                                 cAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCa                                                                                                                <  1:77111/149‑1 (MQ=255)
                                  aGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCaa                                                                                                               >  2:277897/1‑149 (MQ=255)
                                   ggAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAAt                                                                                                              <  7:297320/149‑1 (MQ=255)
                                    gAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACGGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATg                                                                                                             <  8:105197/149‑1 (MQ=255)
                                    gAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATg                                                                                                             >  6:105610/1‑149 (MQ=255)
                                         tCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGa                                                                                                         >  1:391630/1‑148 (MQ=255)
                                             cgcCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGGCAATGCTGATAtt                                                                                                     <  7:22287/148‑1 (MQ=255)
                                               cccAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGc                                                                                                  <  1:277897/149‑1 (MQ=255)
                                                ccAGCGTGAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGata                                                                                                       <  1:314980/143‑1 (MQ=255)
                                                ccAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCa                                                                                                 >  1:238879/1‑149 (MQ=255)
                                                      tCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCtt                                                                                           >  8:146324/1‑149 (MQ=255)
                                                        aGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTcc                                                                                         >  2:389989/1‑149 (MQ=255)
                                                        aGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTcc                                                                                         <  7:56892/149‑1 (MQ=255)
                                                           cccATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGt                                                                                      >  3:174310/1‑149 (MQ=255)
                                                             cATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCg                                                                                    >  1:136181/1‑149 (MQ=255)
                                                             aaTTTCCGCCAGCTCATCCATGCTTAGCCAGCATAGACAGAGATGAATTAATGCCACCAGGACCGGCTCGTCATTGGATGAACCAACGCCAAGACCGCCGCCCATCCGCAACCTCTTGTAAAGGCTGAGATTCGCCCCGCTTCCCGTCg                                                                                    >  4:30758/2‑149 (MQ=255)
                                                                 tCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGaa                                                                                 <  5:27101/148‑1 (MQ=255)
                                                                   cGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGAc                                                                              <  3:135290/149‑1 (MQ=255)
                                                                    gCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTGGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACg                                                                             >  4:194965/1‑149 (MQ=255)
                                                                      cAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGcc                                                                           <  1:205502/149‑1 (MQ=255)
                                                                          tCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCt                                                                       >  5:250312/1‑149 (MQ=255)
                                                                            aTCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGt                                                                     >  6:34183/1‑149 (MQ=255)
                                                                            aTCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGt                                                                     <  5:34183/149‑1 (MQ=255)
                                                                               cATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCt                                                                   <  7:129694/148‑1 (MQ=255)
                                                                                ctGCTTAGCCCGCATTGCCAGAGATGACTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGc                                                                 <  3:242/148‑1 (MQ=255)
                                                                                 tGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGcc                                                                <  3:126155/149‑1 (MQ=255)
                                                                                   cTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCg                                                               >  4:199332/1‑148 (MQ=255)
                                                                                       gCCCGCATTGCAAGAGATGATTTAATGCCCCCAGGACCGGCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGtt                                                          <  8:371334/149‑1 (MQ=255)
                                                                                        cccGCATTGCCAGAGCTGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGttt                                                         <  5:285265/149‑1 (MQ=255)
                                                                                         ccGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGtttt                                                        >  1:123980/1‑149 (MQ=255)
                                                                                             aTTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTtcat                                                     >  3:216571/1‑148 (MQ=255)
                                                                                                gCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATcaa                                                  <  2:391630/148‑1 (MQ=255)
                                                                                                       atgatTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATcgcg                                          >  4:319755/1‑149 (MQ=255)
                                                                                                           tttAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTgc                                      >  5:283402/1‑149 (MQ=255)
                                                                                                           tttAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTgc                                      >  5:259169/1‑149 (MQ=255)
                                                                                                                 gCCACCCGGACAGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGGCTGCCGCAGTTTTCATCAACAATCGCGCGGCGCGAAc                                <  1:339066/149‑1 (MQ=255)
                                                                                                                   caccaGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGa                              >  6:139470/1‑149 (MQ=255)
                                                                                                                    accaGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGAt                             <  7:146324/149‑1 (MQ=255)
                                                                                                                      caGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTc                                                                    >  4:153472/1‑108 (MQ=255)
                                                                                                                      caGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTc                                                                    <  3:153472/108‑1 (MQ=255)
                                                                                                                          aCCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGtt                       <  1:389989/149‑1 (MQ=255)
                                                                                                                            cGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTAt                     <  4:174310/149‑1 (MQ=255)
                                                                                                                                gcgGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTc                 <  4:216571/149‑1 (MQ=255)
                                                                                                                                  ggCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCAt               <  5:105610/149‑1 (MQ=255)
                                                                                                                                      ttGGATGAACCCCCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTc           >  8:286748/1‑149 (MQ=255)
                                                                                                                                      ttGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTc           <  6:250312/149‑1 (MQ=255)
                                                                                                                                         gATGAACCATCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTc                 <  6:225975/140‑1 (MQ=255)
                                                                                                                                         gATGAACCATCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTc                 >  5:225975/1‑140 (MQ=255)
                                                                                                                                         gATGAACCATCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTc                 <  4:323951/140‑1 (MQ=255)
                                                                                                                                         gATGAACCATCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGGTATCTTc                 >  3:323951/1‑140 (MQ=255)
                                                                                                                                         gATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTtcat                                                     >  7:50293/1‑104 (MQ=255)
                                                                                                                                         gATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTtcat                                                     <  8:50293/104‑1 (MQ=255)
                                                                                                                                         gATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCAc        >  7:198357/1‑149 (MQ=255)
                                                                                                                                         gATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCAc        >  1:374106/1‑149 (MQ=255)
                                                                                                                                           tGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGc      >  6:222157/1‑149 (MQ=255)
                                                                                                                                               ccaccGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTc  <  3:319755/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTC  >  NC_000913/1262415‑1262706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑