Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 457,671 A→G 100% E82E (GAA→GAG)  clpX → ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913457,6710AG98.8% 302.9 / ‑4.5 85E82E (GAA→GAG) clpXATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (40/44);  total (40/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGG  >  NC_000913/457525‑457810
                                                                                                                                                  |                                                                                                                                           
gtgtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACa                                                                                                                                           <  6:228291/149‑1 (MQ=255)
 tgtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAg                                                                                                                                          <  6:372487/149‑1 (MQ=255)
 tgtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAg                                                                                                                                          >  7:144692/1‑149 (MQ=255)
 tgtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAg                                                                                                                                          <  2:29482/149‑1 (MQ=255)
   tataTCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGc                                                                                                                                        <  7:162505/149‑1 (MQ=255)
     tatCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCg                                                                                                                                       >  2:191073/1‑148 (MQ=255)
       tCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGaa                                                                                                                                     >  5:133708/1‑148 (MQ=255)
       tCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGaa                                                                                                                                     >  5:142283/1‑148 (MQ=255)
           cgacgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGaaaaaa                                                                                                                                 <  6:189319/148‑1 (MQ=255)
           cgacgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAg                                                                                                                                <  3:253923/149‑1 (MQ=255)
                aaTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTg                                                                                                                           >  3:133718/1‑149 (MQ=255)
                aaTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTg                                                                                                                           <  3:102163/149‑1 (MQ=255)
                 aTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTgg                                                                                                                          <  6:151678/149‑1 (MQ=255)
                        gATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTcg                                                                                                                    <  4:103660/148‑1 (MQ=255)
                         aTTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTcgcg                                                                                                                  <  3:136231/149‑1 (MQ=255)
                             aTGTAACGACATCATTCGCTAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGta                                                                                                               <  1:301152/148‑1 (MQ=255)
                             aTGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGta                                                                                                               <  2:40837/148‑1 (MQ=255)
                               gTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGAACCGCATCGTGAACGCAGTGAGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGATATCGTCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGtata                                                                                                             >  4:89180/1‑148 (MQ=255)
                                tAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATAc                                                                                                            <  6:133708/148‑1 (MQ=255)
                                 aaCGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGGACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGGATACaa                                                                                                          >  6:185576/1‑149 (MQ=255)
                                 aaCGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGaa                                                                                                                                     >  2:371050/1‑122 (MQ=255)
                                 aaCGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGaa                                                                                                                                     <  1:371050/122‑1 (MQ=255)
                                    gACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGTAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCa                                                                                                       >  6:147865/1‑149 (MQ=255)
                                    gACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCa                                                                                                       >  5:260948/1‑149 (MQ=255)
                                     aCATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCAt                                                                                                      >  1:343496/1‑149 (MQ=255)
                                     aCATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCAt                                                                                                      <  3:321064/149‑1 (MQ=255)
                                       atcatTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGt                                                                                                                               <  6:173143/122‑1 (MQ=255)
                                        tcatTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCCACCCCCTGGACGATTACGGTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTa                                                                                                    >  3:264338/1‑148 (MQ=255)
                                        tcatTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTTCTGGCGGTCGCGGTATACAACCATTAc                                                                                                   <  8:144692/149‑1 (MQ=255)
                                              gcgAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGt                                                                                             >  5:97958/1‑149 (MQ=255)
                                              gcgAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGt                                                                                             >  6:89726/1‑149 (MQ=255)
                                                gAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGc                                                                                                                                        <  3:55708/104‑1 (MQ=255)
                                                gAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGc                                                                                                                                        >  4:55708/1‑104 (MQ=255)
                                                  agagATTAATGAAGGTGCACCGCATCGTGATCGCAGTGCGCTACCGACGCCGCATGAAATTCGCATCCACCTGGACGATTACGTTATCGGACAGGAGCAGGCGAATAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTgc                                                                                         <  3:89180/149‑1 (MQ=255)
                                                  agagATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTgc                                                                                         <  6:97958/149‑1 (MQ=255)
                                                      aTTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCaa                                                                                     >  2:275604/1‑149 (MQ=255)
                                                        tAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACg                                                                                   >  4:281223/1‑149 (MQ=255)
                                                         aaaGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACgg                                                                                  >  2:297188/1‑149 (MQ=255)
                                                          aagaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACaaa                                                                                                >  3:88581/1‑134 (MQ=255)
                                                          aagaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACaaa                                                                                                <  4:88581/134‑1 (MQ=255)
                                                          aagaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGc                                                                                 <  1:191073/149‑1 (MQ=255)
                                                          aagaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGc                                                                                 >  6:279525/1‑149 (MQ=255)
                                                              agTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATa                                                                             <  4:337236/149‑1 (MQ=255)
                                                              agTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATa                                                                             <  5:220161/149‑1 (MQ=255)
                                                                  gCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCa                                                                          <  1:275604/148‑1 (MQ=255)
                                                                   cACCGCATCGACAACGCACTGCGCAACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAg                                                                         <  6:299559/148‑1 (MQ=255)
                                                                     ccGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCaa                                                                      >  2:12649/1‑149 (MQ=255)
                                                                      cGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCTCAACGGCGATACCAGCAAt                                                                     >  4:361485/1‑149 (MQ=255)
                                                                      cGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAAt                                                                     <  7:132404/149‑1 (MQ=255)
                                                                      cGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAAt                                                                     >  5:325265/1‑149 (MQ=255)
                                                                       gCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATg                                                                    <  5:107146/149‑1 (MQ=255)
                                                                         aTCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGTCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGc                                                                  <  4:133718/149‑1 (MQ=255)
                                                                         aTCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGc                                                                  <  6:142283/149‑1 (MQ=255)
                                                                            gTGAACGCAGTGCGCTACCGACGCTGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTc                                                               >  4:169247/1‑149 (MQ=255)
                                                                               aaCGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGGCGAg                                                            >  6:27593/1‑149 (MQ=255)
                                                                                      tGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGc                                                      >  3:361536/1‑148 (MQ=255)
                                                                                         gcTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCaaaa                                                  <  2:343496/149‑1 (MQ=255)
                                                                                         gcTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCaaaa                                                  <  5:147865/149‑1 (MQ=255)
                                                                                         gcTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCaaaa                                                  <  8:107805/149‑1 (MQ=255)
                                                                                           tACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATgg                                                                   >  7:56196/1‑130 (MQ=255)
                                                                                           tACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATgg                                                                   <  8:56196/130‑1 (MQ=255)
                                                                                             ccGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCG‑AAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTaa                                              >  4:325892/1‑148 (MQ=255)
                                                                                              cGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAAc                                             <  1:12649/149‑1 (MQ=255)
                                                                                                 cgccgcATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACAtt                                          <  5:279525/149‑1 (MQ=255)
                                                                                                  gccgcATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTc                                         >  4:280686/1‑149 (MQ=255)
                                                                                                      cATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTctgct                                     >  7:203806/1‑149 (MQ=255)
                                                                                                         gAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACACCCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGAt                                  >  2:13920/1‑149 (MQ=255)
                                                                                                         gAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGtt                                                          <  4:20263/125‑1 (MQ=255)
                                                                                                         gAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGtt                                                          >  3:20263/1‑125 (MQ=255)
                                                                                                          aaaTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTTGGCAAAAGTAACATTCTGCTGATc                                 <  3:169247/149‑1 (MQ=255)
                                                                                                           aaTTCGCAACCACCTTGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCg                                <  3:361485/149‑1 (MQ=255)
                                                                                                            aTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACa                                                                                                  <  2:289970/82‑1 (MQ=255)
                                                                                                            aTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACa                                                                                                  >  1:289970/1‑82 (MQ=255)
                                                                                                            aTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCgg                               >  4:119769/1‑149 (MQ=255)
                                                                                                                    ccaccTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCg                       >  7:65631/1‑149 (MQ=255)
                                                                                                                    ccaccTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCg                       >  7:230662/1‑149 (MQ=255)
                                                                                                                       ccTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGtt                    <  8:65631/149‑1 (MQ=255)
                                                                                                                        cTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTc                   >  5:370557/1‑149 (MQ=255)
                                                                                                                        cTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTc                   <  3:197803/149‑1 (MQ=255)
                                                                                                                           gACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGCTCGGTCCGACCGGTTCCgg                >  7:379567/1‑149 (MQ=255)
                                                                                                                           gACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCgg                >  6:91353/1‑149 (MQ=255)
                                                                                                                           gACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCgg                <  1:10802/149‑1 (MQ=255)
                                                                                                                              gATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTaa             <  4:361536/149‑1 (MQ=255)
                                                                                                                                    gTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACgct       >  1:108830/1‑149 (MQ=255)
                                                                                                                                          ggCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTgctgg  <  6:370557/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
GTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGG  >  NC_000913/457525‑457810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑