Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,934,587 T→C 5.9% L6L (TTA→TTG)  edd ← 6‑phosphogluconate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,934,5870TC 5.9% 260.6 / 5.0 85L6L (TTA→TTG) edd6‑phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (35/45);  new base C (3/2);  total (38/47)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

CTGGCTGGCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAA  >  NC_000913/1934442‑1934733
                                                                                                                                                 |                                                                                                                                                  
ctggctggCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGt                                                                                                                                                    <  5:70548/146‑1 (MQ=255)
ctggctggCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTaa                                                                                                                                                  <  3:274065/148‑1 (MQ=255)
   gctggcAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAAGaa                                                                                                                                               <  5:209444/148‑1 (MQ=255)
   gctggcAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAAGaa                                                                                                                                               >  6:209444/1‑148 (MQ=255)
   gcgggcaggcaGCGAAACCGTCTGCCAGGGTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTaata                                                                                                                                                <  2:341783/144‑3 (MQ=255)
     tggcAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATtg                                                                                                                                            >  4:33557/1‑149 (MQ=255)
      ggcaggcaGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATtgt                                                                                                                                           <  8:208014/149‑1 (MQ=255)
       gcaggcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGATCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATtgt                                                                                                                                           <  7:137356/148‑1 (MQ=255)
         aggcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGa                                                                                                                                        >  4:311881/1‑149 (MQ=255)
           gcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGAtt                                                                                                                                      <  3:33557/149‑1 (MQ=255)
             agCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGAGTCGATTTGTTACGCGTAATAATTGTGGATTCa                                                                                                                                    >  2:251384/1‑149 (MQ=255)
               cGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCAt                                                                                                                                   >  3:331941/1‑148 (MQ=255)
                gAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATa                                                                                                                                  >  5:216601/1‑148 (MQ=255)
                  aaCCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAAGAATTGTGGATTCATAAAg                                                                                                                               <  3:47789/149‑1 (MQ=255)
                   acagtgtGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAgg                                                                                                                              <  6:98800/146‑1 (MQ=255)
                            cAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGaaa                                                                                                                     >  8:254077/1‑149 (MQ=255)
                            cAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGaaa                                                                                                                     >  6:250110/1‑149 (MQ=255)
                              ggTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAAtt                                                                                                                   <  6:216601/149‑1 (MQ=255)
                                ttACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGa                                                                                                                 <  5:213939/149‑1 (MQ=255)
                                ttACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGa                                                                                                                 <  8:34545/149‑1 (MQ=255)
                                 tACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAg                                                                                                                >  3:140569/1‑149 (MQ=255)
                                    cgaaTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTg                                                                                                             <  2:320235/146‑1 (MQ=255)
                                      cATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGt                                                                                                            >  3:286029/1‑148 (MQ=255)
                                      cATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTc                                                                                                           <  6:54443/149‑1 (MQ=255)
                                      cATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGt                                                                                                            >  4:288546/1‑148 (MQ=255)
                                       aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGt                                                                                                            >  4:55525/1‑147 (MQ=255)
                                       aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCa                                                                                                          <  4:37795/149‑1 (MQ=255)
                                            aaCTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCa                                                                                                     <  7:220558/149‑1 (MQ=255)
                                            aaCTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAAGAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCa                                                                                                     <  3:318811/149‑1 (MQ=255)
                                            aaCTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCagag                                                                                                       >  6:123261/1‑147 (MQ=255)
                                             aCTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAAGAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAg                                                                                                    >  6:372741/1‑149 (MQ=255)
                                              cTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAgg                                                                                                   <  8:6376/149‑1 (MQ=255)
                                               tGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGga                                                                                                  >  5:104789/1‑149 (MQ=255)
                                               tGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATCGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAg                                                                                                    >  6:185947/1‑147 (MQ=255)
                                               tGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATCGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAg                                                                                                    <  5:185947/147‑1 (MQ=255)
                                                gCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGa                                                                                                                 <  1:264729/133‑1 (MQ=255)
                                                gCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGAATCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGa                                                                                                                 >  2:264729/1‑133 (MQ=255)
                                                 cGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGgat                                                                                                 <  5:318569/148‑1 (MQ=255)
                                                  gAACGATGAACGGTCGAAGTTTTCGCGTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGgatga                                                                                               >  3:16713/1‑149 (MQ=255)
                                                        tGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATTATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTcac                                                                                          >  2:344906/1‑148 (MQ=255)
                                                              gTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGt                                                                                   >  4:51494/1‑149 (MQ=255)
                                                                    gTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcatt                                                                             <  5:305493/149‑1 (MQ=255)
                                                                     ttttCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcatt                                                                             <  4:140569/148‑1 (MQ=255)
                                                                       ttCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcattca                                                                           <  3:311881/148‑1 (MQ=255)
                                                                         cGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGTGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCaga                                                                         <  7:20406/148‑1 (MQ=255)
                                                                          gCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcattc                                                                            <  1:133200/144‑1 (MQ=255)
                                                                          gCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcattc                                                                            >  2:133200/1‑144 (MQ=255)
                                                                            ttGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGc                                                                                                      >  4:162560/1‑116 (MQ=255)
                                                                                 cTATCCGGGCGAGATAAGCAGATCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCGTGAAATTGAGTTGTCAGAGCAGGATTATTCACAACGCGTTTCATTCAGAGTATTTAt                                                                 <  6:355137/148‑1 (MQ=255)
                                                                                    tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGga                                                                                                  >  6:287947/1‑112 (MQ=255)
                                                                                    tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGga                                                                                                  <  5:287947/112‑1 (MQ=255)
                                                                                    tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACt                                                             <  7:326457/149‑1 (MQ=255)
                                                                                       gggCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGa                                                           >  2:329155/1‑148 (MQ=255)
                                                                                          cGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACg                                                       >  3:63930/1‑149 (MQ=255)
                                                                                             gaTAAGCAGAGCGAGGCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAATGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTGCATTCAGAGGATTTATGACTGAAACGCCt                                                    <  4:28437/149‑1 (MQ=255)
                                                                                              aTAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTg                                                   >  6:364805/1‑149 (MQ=255)
                                                                                                aaGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGt                                                  >  1:273363/1‑148 (MQ=255)
                                                                                                  gCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTAAGAGGATTTATGACTGAAACGCCTGTacc                                               >  1:307392/1‑147 (MQ=255)
                                                                                                   cAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAAcc                                              >  6:205762/1‑149 (MQ=255)
                                                                                                    agagCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCTTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACATCGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCg                                             <  4:339280/149‑1 (MQ=255)
                                                                                                    agagCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTTATTCAGAGGATTTATGACTGAAACGCCTGTAACCg                                             <  1:182975/149‑1 (MQ=255)
                                                                                                     gagCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGAATGAAACGCCTGTAACCg                                             >  5:104774/1‑148 (MQ=255)
                                                                                                         gAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATTACTGAAACGCCTGTAACCg                                             >  2:304432/1‑144 (MQ=255)
                                                                                                         gAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGGCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCg                                             <  1:304432/144‑1 (MQ=255)
                                                                                                            tctcGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCAt                                     <  5:123261/149‑1 (MQ=255)
                                                                                                             ctcGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCa                                      >  7:328851/1‑147 (MQ=255)
                                                                                                                tcgcgAACGTTCAATGAATCGATTTGTTCAGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATGCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAg                                   <  6:222587/146‑1 (MQ=255)
                                                                                                                gggcgAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATa                                    <  5:250110/144‑1 (MQ=255)
                                                                                                                gcgcgAACGTTCAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAg                                   <  2:301107/147‑1 (MQ=255)
                                                                                                                           cAATGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGtt                       <  8:225723/148‑1 (MQ=255)
                                                                                                                            aaTGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGttt                      <  8:328851/148‑1 (MQ=255)
                                                                                                                            aaTGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGttt                      <  4:63930/148‑1 (MQ=255)
                                                                                                                            aaTGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtc                     <  1:329155/149‑1 (MQ=255)
                                                                                                                            aaTGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtc                     <  7:254077/149‑1 (MQ=255)
                                                                                                                            aaTGATTCGATTTGTTACGCGTAATAATTGTGGAGTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATTACTGAAACCCCTCTAACCGGAGCACATACGGCAAAAACGttt                      >  1:345020/1‑148 (MQ=255)
                                                                                                                             aTGATTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGttt                      >  4:361106/1‑147 (MQ=255)
                                                                                                                             aTGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAg                                   >  3:306912/1‑134 (MQ=255)
                                                                                                                             aTGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAg                                   <  4:306912/134‑1 (MQ=255)
                                                                                                                                aTTCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt                  >  7:226830/1‑148 (MQ=255)
                                                                                                                                 ttCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagtca                <  3:361106/149‑1 (MQ=255)
                                                                                                                                 ttCGATTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt                  >  6:326839/1‑147 (MQ=255)
                                                                                                                                    gATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGTATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAgtg             <  3:288546/149‑1 (MQ=255)
                                                                                                                                     aTTTGTTACGCGTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAgtg             >  1:77253/1‑148 (MQ=255)
                                                                                                                                        tGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGtaa          <  6:104789/148‑1 (MQ=255)
                                                                                                                                              gcgTAAGAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATaaaaaa   <  5:372741/149‑1 (MQ=255)
                                                                                                                                               cgTAATAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATaaaaaaa  <  5:364805/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTGGCTGGCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAA  >  NC_000913/1934442‑1934733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑