Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,100,944 | T→C | 6.4% | I109V (ATT→GTT) | gnd ← | 6‑phosphogluconate dehydrogenase, decarboxylating |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,100,944 | 0 | T | C | 6.4% | 188.0 / 3.2 | 62 | I109V (ATT→GTT) | gnd | 6‑phosphogluconate dehydrogenase, decarboxylating |
Reads supporting (aligned to +/- strand): ref base T (31/27); new base C (2/2); total (33/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01 |
TCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAAC > NC_000913/2100800‑2101092 | tCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGAAGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAgtg > 3:119961/1‑149 (MQ=255) gcAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAACCACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAgag > 5:257609/2‑147 (MQ=255) aGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTc < 8:253704/149‑1 (MQ=255) gATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCt > 4:68296/1‑149 (MQ=255) aTCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTg < 1:130522/149‑1 (MQ=255) aTCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTg < 3:68296/149‑1 (MQ=255) gTGCTACCAATTCATAGGCTTCTTTCTGGCCCCCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTgg > 6:211895/1‑146 (MQ=255) cTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGaagaag < 3:137391/149‑1 (MQ=255) aCCAATTCATAGGCTTCTATCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGgt > 8:188100/1‑149 (MQ=255) cATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTacc < 4:333467/148‑1 (MQ=255) aGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTAccacca > 7:199272/1‑149 (MQ=255) tGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatgat > 1:387165/1‑149 (MQ=255) gCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatgat < 5:211895/147‑1 (MQ=255) ccaccaGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCCCCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGtc > 5:347008/1‑149 (MQ=255) caGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCt > 5:322448/1‑149 (MQ=255) aGGCATAATAGAAGGACCTTTCAGCGCTCGCTCTTCACCGCCAGAAAGACCGGTACCGATGAAGTTAATGCCCGCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCtt < 4:21001/149‑1 (MQ=255) aGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCtt > 7:290794/1‑149 (MQ=255) aataGAAGGACCTTTCAGCGCCACCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTAACCATTACGACGAATAGTCTCCTTGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTGTATc > 5:296267/1‑147 (MQ=255) ataGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCgag < 8:290794/149‑1 (MQ=255) ataGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCga < 4:239271/148‑1 (MQ=255) aaGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTa > 1:203143/1‑140 (MQ=255) aaGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTa < 2:203143/140‑1 (MQ=255) aGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAACAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGtctc > 8:31041/1‑134 (MQ=255) aGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAACAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGtctc > 4:187169/1‑134 (MQ=255) aGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAACAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGtctc < 7:31041/134‑1 (MQ=255) aGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAACAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGtctc < 3:187169/134‑1 (MQ=255) ggACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATgg > 1:392450/1‑149 (MQ=255) ccTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGttt < 8:199272/149‑1 (MQ=255) cTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGatatagatcg > 3:327394/1‑143 (MQ=255) cTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGata < 4:327394/142‑1 (MQ=255) cTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGttt > 1:342599/1‑148 (MQ=255) cAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAACGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAgg < 6:134823/148‑1 (MQ=255) ccccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCaa > 1:302077/1‑149 (MQ=255) cccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCaa > 2:39419/1‑148 (MQ=255) cccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGAGTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCaa > 4:191365/1‑148 (MQ=255) tctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCAGGATTACGACGAATAGTGTCCTGGAAGAAGGAGTTACCACCATCAATGATCATGTCTCCTTTATCGAGATATGGTATGAGGCAATCAATag > 2:7045/1‑148 (MQ=255) tctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGTCGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATagc > 6:13298/1‑149 (MQ=255) cACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAgcagca > 1:110209/1‑149 (MQ=255) ccGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATc > 6:59837/1‑149 (MQ=255) ccAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGt < 7:188100/149‑1 (MQ=255) aGAAACACCGGTACCGATGAAGTTACAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGc < 2:302077/149‑1 (MQ=255) acCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAg < 7:66172/120‑1 (MQ=255) acCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAg > 8:66172/1‑120 (MQ=255) cGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTTTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCt < 4:119961/149‑1 (MQ=255) cGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGtct > 1:85572/1‑95 (MQ=255) cGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGtct < 2:85572/95‑1 (MQ=255) gTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGc < 2:392450/149‑1 (MQ=255) cGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTc < 6:322448/149‑1 (MQ=255) aGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCAtt < 1:198244/149‑1 (MQ=255) aaaGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAAc < 6:347008/148‑1 (MQ=255) aaaGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACAATTaa > 3:287824/1‑147 (MQ=255) ccTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatgat < 4:130974/70‑1 (MQ=255) cTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGCTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGttt > 2:23462/1‑149 (MQ=255) cTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTATCCTTTATAGAGATATGGTTTGAGGGAATCAATAGCAGCATACGTGCCTTCACCTGCTTTCACCATTAACAGGATGCGACGAGGAGttt > 3:4617/1‑149 (MQ=255) tCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTc > 2:118861/1‑149 (MQ=255) aCGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTc < 5:59837/147‑1 (MQ=255) aCGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCa < 2:342599/149‑1 (MQ=255) ttACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGtt > 2:260399/1‑73 (MQ=255) ttACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGtt < 1:260399/73‑1 (MQ=255) cgacgaATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGAtt < 1:39419/148‑1 (MQ=255) gaATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGAc > 5:248114/1‑148 (MQ=255) aTAGTGTCCTGGAAGAAGGTGTTAACACCATCAATGATGATGTATCCTTTATCGAGATATGGTTGGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGCATGCGACGAGGCGTTTCCAGAGATTCGACaaa > 2:275858/1‑149 (MQ=255) tAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGGCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAAc < 2:382116/149‑1 (MQ=255) | TCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAAC > NC_000913/2100800‑2101092 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |