Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,229,172 C→T 5.5% intergenic (+107/+266) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,229,1720CT 5.5% 211.9 / 2.9 73intergenic (+107/+266)yohP/dusCuncharacterized protein/tRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base C (34/35);  new base T (2/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

GCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCC  >  NC_000913/2229026‑2229317
                                                                                                                                                  |                                                                                                                                                 
gCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAgcgc                                                                                                                                                 <  7:215869/149‑1 (MQ=255)
   aCTGGTGGTGACTGGCGAATTTAAGATGATATTTTAAAATTAATTAATCTCATCAGGTCCGAAAATAACGAGAAAATTTCAGTCTCTCATCCTGTTGCGCTCCAGACATGTGCATTGCTTCATATAATCACTCGCGCAAGGAgcgccc                                                                                                                                               >  8:384755/1‑146 (MQ=255)
     tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAgg                                                                                                                                            >  5:171752/1‑149 (MQ=255)
          gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcg                                                                                                                                       >  5:273567/1‑149 (MQ=255)
          gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTACGCTCCTGTCATGTGCGTTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcg                                                                                                                                       >  5:28040/1‑149 (MQ=255)
            gACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggggcggc                                                                                                                                      >  2:86239/1‑144 (MQ=255)
            gACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggggcggc                                                                                                                                      >  2:81059/1‑144 (MQ=255)
             aCTGGCGTATTTAAGATGATATTTTAAAATTAATTAATCTCATCAGGGCCGAAAATAACGAGAATATTTCAGTCTCTCAGCCTGTTGCGCTCCTGTCATGTGCCTTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcggc                                                                                                                                     <  8:318155/148‑1 (MQ=255)
               tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCaa                                                                                                                                  >  3:258958/1‑149 (MQ=255)
               tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGCCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCaa                                                                                                                                  >  1:216397/1‑149 (MQ=255)
                 gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATc                                                                                                                                <  4:258958/149‑1 (MQ=255)
                   gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGTGCGCAGGGGGCGGCCAATcgc                                                                                                                              >  1:144796/1‑149 (MQ=255)
                   gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGTGCGCAGGGGGCGGCCAATcgc                                                                                                                              >  1:139484/1‑149 (MQ=255)
                    tATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgcc                                                                                                                             <  2:346093/149‑1 (MQ=255)
                      tttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGTCAATcgccgc                                                                                                                           <  8:363636/149‑1 (MQ=255)
                       ttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATc                                                                                                                                >  8:365773/1‑143 (MQ=255)
                       ttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATc                                                                                                                                <  7:365773/143‑1 (MQ=255)
                          acatgatATTATAAACTTAATTACTGTCATCACGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccgccgc                                                                                                                        <  3:209875/146‑1 (MQ=255)
                          aGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcg                                                                                                                               >  6:151449/1‑141 (MQ=255)
                          aGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcg                                                                                                                               <  5:151449/141‑1 (MQ=255)
                           gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGccc                                                                                                                      >  1:206929/1‑149 (MQ=255)
                                  ttttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGCGCGCGCAGGGGGCGGGCAATCGCCGCCGCCCCCTGccc                                                                                                               >  2:111023/1‑147 (MQ=255)
                                  ttttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAATCGCCCCCGCCCCCGGcgc                                                                                                               >  4:188439/1‑147 (MQ=255)
                                       aaaTTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCg                                                                                                          >  5:310912/1‑149 (MQ=255)
                                           taattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCTCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATAGCCGCCGCCCCCTGCACCCCCGGGct                                                                                                      >  3:288192/1‑149 (MQ=255)
                                                aatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGc                                                                                                 <  1:86239/148‑1 (MQ=255)
                                                aatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGc                                                                                                 <  1:81059/148‑1 (MQ=255)
                                                 atGTCATCAGGTCCGAAAATAACGAGAATATTTCAGGCTCTCATCCTGTTGCGCTCCTGTCATGGGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGc                                                                                                 <  5:314657/148‑1 (MQ=255)
                                                  tGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCCCCGCCCCCTGCCCCCCCGGGCACAGGCg                                                                                                >  6:282393/1‑148 (MQ=37)
                                                    tcatcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCGCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGaa                                                                                              <  1:111023/148‑1 (MQ=255)
                                                      atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGGGGCCCATACCCGCCGCCCCCTGCCCCCCCCGGCACAGGCGAACa                                                                                            >  3:61164/1‑148 (MQ=255)
                                                      ataaGGTCCGAAAATAACGACAATATTTCAGTCTCTCATCCTTTTGCGCTCCTGTCATGTGCATTTCTTAATATAATCACTGGCGCATGGCGCGCGCAGGGGGCGGCCAATCGCGGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACaa                                                                                           <  8:62658/146‑1 (MQ=25)
                                                         aGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCCGCCCAACGCCGCCCCCCCCTGCACCCCCCGGCTCTCGCCCACaaaa                                                                                         >  4:53278/1‑148 (MQ=25)
                                                          ggTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATc                                                                                       >  3:106621/1‑149 (MQ=255)
                                                            tCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgc                                                                                     <  6:273567/149‑1 (MQ=255)
                                                               gAAAATAACGAGAATATTTCAGTCTCTGATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGCAGCGCCCCACGGGCGGCCAAACTCCACCCCCCCCAGCCCCCCCCGGCTAGAGCGAAAAAAAAcgacc                                                                                   >  6:31692/1‑145 (MQ=255)
                                                               gAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCCGGGGGGGCCAAAACCCCCCCCCCCCAGCACCCCCCCGCTCGGGCGCACaaaaaaacccc                                                                                  >  6:213906/1‑142 (MQ=25)
                                                                 aaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAACCCACCGCCCCCAGCGCCCCCCGGCGCTGGCGACAAAAAAcgccgcgg                                                                                >  5:209243/1‑147 (MQ=25)
                                                                       cGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGt                                                                          <  3:314329/149‑1 (MQ=255)
                                                                       cGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGt                                                                          <  7:384755/149‑1 (MQ=255)
                                                                          gaATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGcc                                                                       >  1:293827/1‑149 (MQ=255)
                                                                               tttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                  <  2:293827/149‑1 (MQ=255)
                                                                               tttCAGACTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGGGGCCAATAGCCCCCGCCCCCGGCCCCCCCCGGCGCGGGCGGACCAAATAGCCGCGGCGCGGGGGCCGCgg                                                                  >  6:212240/1‑149 (MQ=18)
                                                                                 tCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                 <  6:171752/148‑1 (MQ=255)
                                                                                    gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt                                                             <  7:116186/149‑1 (MQ=255)
                                                                                     tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATc                                                            <  5:282393/149‑1 (MQ=255)
                                                                                     tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATc                                                            <  5:47657/149‑1 (MQ=255)
                                                                                         tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGTGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                           <  2:144796/146‑1 (MQ=255)
                                                                                         tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGTGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                           <  2:139484/146‑1 (MQ=255)
                                                                                         tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATccc                                                          <  6:310912/147‑1 (MQ=255)
                                                                                         tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCt                                                         >  1:215615/1‑148 (MQ=255)
                                                                                           aTCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCCGGGGCGGCCAAAAGCCCCCCCCCCCAGCACCCCCCGGCGCTGGCGCACAAAAAAGCCCCAGCGCCGTGCCCACCGCTTaaaacat                                                        >  4:4539/1‑141 (MQ=16)
                                                                                               tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGc                                                   >  8:307728/1‑148 (MQ=255)
                                                                                                   gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAcc                                               <  5:213906/148‑1 (MQ=255)
                                                                                                    cgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCgg                                             <  3:53278/149‑1 (MQ=255)
                                                                                                      cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGt                                           <  3:4539/149‑1 (MQ=255)
                                                                                                              aTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACCAAAACGCCGCGGCGCGGTGCCCTCGGCGTATCCCGTACGCCTACCGGGTCGGGcgcg                                   >  3:334409/1‑149 (MQ=17)
                                                                                                                   cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCCCCCCCAGCCCCCCCCGGCTCTGGCGAACAAAATCGCCCCGGCGCGGTGCCCTCGGCTTATCCCATACCGCTACCCGGTAGGgccgcaggg                               >  6:165480/1‑141 (MQ=14)
                                                                                                                    aTTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                             <  4:61164/149‑1 (MQ=255)
                                                                                                                    aTTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGAACAAACTCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                             <  7:307728/149‑1 (MQ=255)
                                                                                                                        cTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATc                         <  2:216397/149‑1 (MQ=40)
                                                                                                                        cTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATAGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGCACAAAATCGCCGCGGCGCGGTGCCCTCGGCGTATACCTTACGGCTACCGGGTCGGGCGCGAGGTAACCTc                         >  3:90294/1‑149 (MQ=14)
                                                                                                                          tCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATccc                       >  2:312093/1‑149 (MQ=39)
                                                                                                                           cATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                        <  5:212240/147‑1 (MQ=38)
                                                                                                                           cATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                        <  4:296047/147‑1 (MQ=38)
                                                                                                                           cATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                      >  2:288229/1‑149 (MQ=38)
                                                                                                                            atatAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                      <  7:331254/148‑1 (MQ=38)
                                                                                                                            atatAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                      <  3:112430/148‑1 (MQ=38)
                                                                                                                                aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATAGCCGCCGCCCCCTGCCCCCCCGGGCGCTGGCGCACAAAATAGCCGCTGCGCGGTGCCCAACGCTTATACCATACGGCTACCCGGACGGGCGCGAGGTAACATACCTGtta                  >  6:371311/1‑146 (MQ=11)
                                                                                                                                aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATAGCCGCCGCCCCCTGCCAACCCGGGCTCTGGCGAAAAAAATAGCCGCTGCGCGGTGCCCTAGGCTTATACCTTACTGCTACCGGGTCGGGCGCGAGGTAAAATACCTGTaa                  >  4:307904/1‑148 (MQ=11)
                                                                                                                                  tCACTGGGGCATGGAGCGCGCAGGGGGCGGCCAATCGACGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAc               <  6:209243/149‑1 (MQ=12)
                                                                                                                                        gcgcAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCGGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGCGGGGACAGCCCTGTACAACGCGcc          >  1:270187/1‑148 (MQ=11)
                                                                                                                                        gcgcAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc           <  6:28040/147‑1 (MQ=17)
                                                                                                                                            aaGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAg     <  6:270645/149‑1 (MQ=32)
                                                                                                                                               gAGCGCGCAGGGGGCGGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGccc  <  3:188439/149‑1 (MQ=17)
                                                                                                                                                  |                                                                                                                                                 
GCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCC  >  NC_000913/2229026‑2229317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: