Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,462,677 A→T 5.6% intergenic (+31/‑335) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,462,6770AT 5.6% 344.2 / 8.1 108intergenic (+31/‑335)fadL/yfdFlong‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (50/51);  new base T (2/4);  total (52/56)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

Only 100 of 109 total aligned reads displayed.

GACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT  >  NC_000913/2462530‑2462824
                                                                                                                                                   |                                                                                                                                                   
gACGTTGGTGTTTATTATATGGAAGGTCAGAGCGTGATAATTTACGAAGGCCCATACCAGGTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCTCCTGCa                                                                                                                                                     <  4:82849/148‑1 (MQ=255)
 aCGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATa                                                                                                                                                   <  3:279372/149‑1 (MQ=255)
   gTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAg                                                                                                                                                  >  3:71660/1‑148 (MQ=255)
    ttGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAg                                                                                                                                                  >  2:109352/1‑147 (MQ=255)
       gtgtgtCTTCTATGCACGGTCCGAGAGTGAAAATTAACGAACGCCCATACCATTTCGAGTCTGAATGTAAAGCCTGTCTGTTCGGTACTAACTTTAAATACGCGTTCTGATAACGCGTTCGCCTTGATAAAGTCACCGGCATAGCAGGt                                                                                                                                             <  6:254075/149‑1 (MQ=255)
       gtgtTTCTTATATGCACGGTGAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTTTTCGGTACTACCTTTAACTACGCGTTCTCATAACGCGTTCGCATGGATAAAGTCACCTGAATAGCAgtt                                                                                                                                             >  2:147979/1‑147 (MQ=255)
        tgtTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGTAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTg                                                                                                                                            <  6:105858/149‑1 (MQ=255)
        tggttCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGGTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTg                                                                                                                                            <  6:253336/146‑1 (MQ=255)
          tttCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGa                                                                                                                                           <  4:127512/148‑1 (MQ=255)
            tCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGACt                                                                                                                                         <  8:132860/148‑1 (MQ=255)
            tCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACt                                                                                                                                         <  6:64140/148‑1 (MQ=255)
            tCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACt                                                                                                                                         <  7:269684/148‑1 (MQ=255)
             cTTATATGCACGGTCAGAGCGGGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGGGACtt                                                                                                                                        <  6:80517/148‑1 (MQ=255)
              ttATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACttt                                                                                                                                       >  1:300566/1‑148 (MQ=255)
               tataTGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACtt                                                                                                                                        <  3:321704/146‑1 (MQ=255)
               tataTGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACtt                                                                                                                                        >  4:321704/1‑146 (MQ=255)
                atatGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGCATAAAGTCACCTGCATAGCAGGTGACTTTaa                                                                                                                                     <  2:305087/148‑1 (MQ=255)
                 tatGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAAc                                                                                                                                    <  2:263838/148‑1 (MQ=255)
                 tatGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAAc                                                                                                                                    <  7:23522/148‑1 (MQ=255)
                    gCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTccc                                                                                                                                >  7:255406/1‑149 (MQ=255)
                          tCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACtt                                                                                                                          <  8:283049/149‑1 (MQ=255)
                             gagCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCAc                                                                                                                       <  2:227246/149‑1 (MQ=255)
                                  tGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGaaa                                                                                                                  <  4:78369/149‑1 (MQ=255)
                                  ggAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACGACCTTTAACTACGCGTTCTGCTACCGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGCCTTTAACTCCCCCACTTCCCCGa                                                                                                                    >  7:271749/2‑147 (MQ=255)
                                   gAAAATTACCGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAg                                                                                                                                               <  6:33023/119‑1 (MQ=255)
                                   gAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGaaa                                                                                                                  <  1:80467/148‑1 (MQ=255)
                                   gAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGa                                                                                                                    >  2:80467/1‑146 (MQ=255)
                                   gAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAag                                                                                                                 <  8:228986/149‑1 (MQ=255)
                                     aaaTTAACGAACGCCCATACCAGTTCGAGTCTGAAGGGAAAGCCTGGCTGTTCGGTACTAACATTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAagt                                                                                                                <  8:152594/148‑1 (MQ=255)
                                       aTTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAagtagt                                                                                                             <  2:265935/149‑1 (MQ=255)
                                            cGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGACt                                                                                                                                         >  7:71700/1‑116 (MQ=255)
                                            cGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGACt                                                                                                                                         <  8:71700/116‑1 (MQ=255)
                                             gAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAagtagt                                                                                                             <  7:130035/143‑1 (MQ=255)
                                             gAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAagtagt                                                                                                             >  8:130035/1‑143 (MQ=255)
                                                 gCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAagta                                                                                                               >  3:44007/1‑137 (MQ=255)
                                                   ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCt                                                                                                 >  6:23492/1‑149 (MQ=255)
                                                   ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCt                                                                                                 >  8:172918/1‑149 (MQ=255)
                                                      tACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCa                                                                                                                                                >  5:118714/1‑99 (MQ=255)
                                                       agcAGTTCGAGTGTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCCTAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTc                                                                                                          <  8:308667/134‑1 (MQ=255)
                                                           gTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTAcctcct                                                                                         >  1:172729/1‑149 (MQ=255)
                                                               gAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGACTTTa                                                                                                                                      >  1:45164/1‑100 (MQ=255)
                                                               gAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGt                                                                                     >  6:337478/1‑149 (MQ=255)
                                                                 gTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGttt                                                                                   <  8:255406/149‑1 (MQ=255)
                                                                      aaGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGc                                                                                <  2:300566/147‑1 (MQ=255)
                                                                        ggTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCaaa                                                                             >  7:200482/1‑148 (MQ=255)
                                                                         gTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCaaa                                                                             >  7:316845/1‑147 (MQ=255)
                                                                           aaaGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAAc                                                                            >  4:198166/1‑146 (MQ=255)
                                                                           aaaGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAAc                                                                            <  3:198166/146‑1 (MQ=255)
                                                                              gCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTg                                                                      >  1:12751/1‑149 (MQ=255)
                                                                                cTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGtt                                                                    >  5:24890/1‑149 (MQ=255)
                                                                                     tGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAg                                                               >  7:317123/1‑149 (MQ=255)
                                                                                      gTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGc                                                              >  3:12473/1‑149 (MQ=255)
                                                                                         cGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCtt                                                            <  5:23492/148‑1 (MQ=255)
                                                                                              cTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCt                                                       <  7:22848/148‑1 (MQ=255)
                                                                                               tAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCtt                                                      >  8:395461/1‑148 (MQ=255)
                                                                                                aaCTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCttt                                                     >  3:297547/1‑148 (MQ=255)
                                                                                                 aCTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATTGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTc                                                    <  7:25881/148‑1 (MQ=255)
                                                                                                 aCTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTcccc                                                                                                                               <  4:342828/73‑1 (MQ=255)
                                                                                                 aCTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTcccc                                                                                                                               >  3:342828/1‑73 (MQ=255)
                                                                                                   tttAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCaaa                                                 >  8:383165/1‑149 (MQ=255)
                                                                                                   tttAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACAGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCaaa                                                 >  5:36349/1‑149 (MQ=255)
                                                                                                   caggcgaaCGCGTTATCAGAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCaaa                                                 >  7:80574/8‑149 (MQ=255)
                                                                                                    aggcgaaCGCGTTATCAGAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAAt                                                >  8:73679/7‑149 (MQ=255)
                                                                                                         tACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCaaa                                                 >  8:218440/1‑143 (MQ=255)
                                                                                                         tACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCaaa                                                 <  7:218440/143‑1 (MQ=255)
                                                                                                         tACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTACTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAAt                                            >  3:243901/1‑148 (MQ=255)
                                                                                                           cgcgTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATaa                                          <  4:297547/148‑1 (MQ=255)
                                                                                                            gcgTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGc                                        >  3:234537/1‑149 (MQ=255)
                                                                                                            gcgTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGc                                        <  7:172918/149‑1 (MQ=255)
                                                                                                            gcgTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGc                                        <  3:266968/149‑1 (MQ=255)
                                                                                                            gcgTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTAACTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGc                                        >  5:273861/1‑149 (MQ=255)
                                                                                                             cgTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCg                                       <  6:24890/149‑1 (MQ=255)
                                                                                                              gTTCTGATAACTCGTTCGTCTGGTTAAAGCAACCTTGATAGCTGGTGTCTTTAACTCCCCCTCTTCTCCTAAATTCGTGTCCTCATTGATTACCTGCTGAGCTTTGCAAACAGCCTGTTGGCAGCTTGCCGTTTCAAATCAATAAGc                                        <  1:147979/147‑1 (MQ=255)
                                                                                                              gTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAAc                                                                            >  1:25247/1‑111 (MQ=255)
                                                                                                              gTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAAc                                                                            <  2:25247/111‑1 (MQ=255)
                                                                                                              gTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGccc                                                        <  1:260991/131‑1 (MQ=255)
                                                                                                              gTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGccc                                                        >  2:260991/1‑131 (MQ=255)
                                                                                                                   gATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGt                                     <  8:116641/145‑1 (MQ=255)
                                                                                                                   gATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATc                                  >  5:28572/1‑148 (MQ=255)
                                                                                                                    atatCGAGTTCGACTGGATAAAGTCTCCTTCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGATTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCGTTTCAAATCAATAAGCGGTAt                                   <  8:316845/146‑1 (MQ=255)
                                                                                                                       aCGCGTTCGGCTGGATAAAGTCCCCTGCATAGCAGGTGACTTTAACTCCCCCACATCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAg                             <  1:237063/149‑1 (MQ=255)
                                                                                                                         gcgTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGATTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGa                            >  3:304198/1‑148 (MQ=255)
                                                                                                                                 cTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGa                   <  6:28572/149‑1 (MQ=255)
                                                                                                                                 cTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCGCCTGAGTTTTGCAAACCGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACCGACAAACCTGa                   >  3:228134/1‑149 (MQ=255)
                                                                                                                                  tGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCGTTTCAAATCAATAAGCGGTATCCACAGACAAACCTg                    >  8:363898/1‑147 (MQ=255)
                                                                                                                                  tGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTg                    >  1:19518/1‑147 (MQ=255)
                                                                                                                                    gATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGaa                  <  6:36349/147‑1 (MQ=255)
                                                                                                                                    gATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGaa                  <  7:395461/147‑1 (MQ=255)
                                                                                                                                     aTAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAgg                >  6:266099/1‑148 (MQ=255)
                                                                                                                                     aTAAAGTCACCTGCATAGCAGGTGACTTTAACTCAACAACTTAACCGAAAGTAGTGTCCTCATTTCTTACCTCCTGATTTTTTCAAACATCCTGTTGGCATCTTGCCCTTTCAAATCAATAAGCTGTATCAACAGACAAACCTGAAgg                >  2:206509/1‑148 (MQ=255)
                                                                                                                                      tAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAggg               >  2:370699/1‑148 (MQ=255)
                                                                                                                                         aGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAg           >  3:109889/1‑149 (MQ=255)
                                                                                                                                         aGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAg           <  5:266099/149‑1 (MQ=255)
                                                                                                                                         aGTCAACTGCATATAAGTTTACTTTAACTCCCACTCTTCACCGAAAGTAGTGTCCTCATTTCTTACCTACTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGTTATACACAGACAAACCTGAAGGTaaat           <  1:206509/149‑2 (MQ=255)
                                                                                                                                             aCCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGc                                                          >  4:318909/1‑98 (MQ=255)
                                                                                                                                             aCCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCat       <  4:12473/149‑1 (MQ=255)
                                                                                                                                             aCCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCa        >  7:304367/1‑148 (MQ=255)
                                                                                                                                             aCCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACAGAAAGTAGTGTCCTCAATGCTTACCTCCTGAGTTTTGCAAACAGCCTGATGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCa        >  2:222267/1‑148 (MQ=255)
                                                                                                                                              ccTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCata      <  5:337478/149‑1 (MQ=255)
                                                                                                                                                  cATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACGTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCGTTTCAAATCAATAGGCGGTATCCACAGCCACACCTGAAGGGAAAGGCATAtttt  >  8:100124/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT  >  NC_000913/2462530‑2462824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: