Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,945,005 A→G 5.6% intergenic (+20/+31) csdE → / ← tcdA CsdA‑binding activator; Fe‑S protein/tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,945,0050AG 5.6% 226.2 / 3.0 71intergenic (+20/+31)csdE/tcdACsdA‑binding activator; Fe‑S protein/tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA
Reads supporting (aligned to +/- strand):  ref base A (36/31);  new base G (2/2);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.10e-01

TTGCAGGCACAGTCACCACTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGC  >  NC_000913/2944860‑2945153
                                                                                                                                                 |                                                                                                                                                    
ttGCAGGCACAGTCACCACTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGa                                                                                                                                                   >  2:235790/1‑149 (MQ=255)
       ctcaGTCACCTCTGTCATTGTTTTATGAGCTGTGATTACGTTCGCAGCTTAGCTCCTCACGCAGCCAGGGGTTATTTGCGTTAAGCGAGGGGATTATCGGTGCGACGAAGCAGGTTTAAGCACATAACCGGGGCGGATAAGACGTTcgc                                                                                                                                            <  4:228967/147‑1 (MQ=255)
          aGTCACCACTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc                                                                                                                                         <  8:75333/149‑1 (MQ=255)
               ccacTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATc                                                                                                                                    >  3:152975/1‑149 (MQ=255)
                     gCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt                                                                                                                              >  1:315049/1‑149 (MQ=255)
                          gTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGAtt                                                                                                                          <  2:292820/148‑1 (MQ=255)
                           tttGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCAGACCTGATTGt                                                                                                                        >  4:297706/1‑149 (MQ=255)
                             tgatgaGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGtt                                                                                                                       <  3:1617/148‑1 (MQ=255)
                                  aGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAcc                                                                                                                  <  3:285386/148‑1 (MQ=255)
                                    cTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCt                                                                                                                >  1:112555/1‑148 (MQ=255)
                                     tGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGa                                                                                                              >  7:61271/1‑149 (MQ=255)
                                                 cgcAGCTTAGCGCCTCACGCAGCCAGTGGTTAAATGCTTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTc                                                                                                  <  3:297706/149‑1 (MQ=255)
                                                   cAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGc                                                                                                >  8:44679/1‑149 (MQ=255)
                                                      cTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATGAGACGCGAACGTCTTATCCGAccc                                                                                                                              <  2:375403/116‑2 (MQ=38)
                                                      cTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATGAGACGCGAACGTCTTATCCGAccc                                                                                                                              >  1:375403/1‑115 (MQ=38)
                                                       ttAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGa                                                                                                                            <  4:155089/117‑1 (MQ=255)
                                                       ttAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGa                                                                                                                            >  3:155089/1‑117 (MQ=255)
                                                         aGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGTcatcat                                                                                          >  3:62562/1‑149 (MQ=255)
                                                         aGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCcatcat                                                                                          >  1:330729/1‑149 (MQ=255)
                                                          gcgcCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCatcatc                                                                                         <  7:44679/149‑1 (MQ=255)
                                                             ccTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATACGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                      >  7:1200/1‑149 (MQ=255)
                                                               tCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTc                                                                                                  <  1:155188/135‑1 (MQ=255)
                                                               tCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTc                                                                                                  >  2:155188/1‑135 (MQ=255)
                                                                        cAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCAt                                                                            >  7:199524/1‑148 (MQ=255)
                                                                         aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                      >  2:247662/1‑137 (MQ=255)
                                                                         aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                      <  1:247662/137‑1 (MQ=255)
                                                                         aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCa                                                                                  <  1:11682/141‑1 (MQ=255)
                                                                         aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCa                                                                                  >  2:11682/1‑141 (MQ=255)
                                                                         aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                          <  1:235790/149‑1 (MQ=255)
                                                                         aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                          <  4:152975/149‑1 (MQ=255)
                                                                         aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                          <  2:112555/149‑1 (MQ=255)
                                                                                 aaTGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCg                                                                   >  1:387062/1‑148 (MQ=255)
                                                                                   tGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGcaa                                                                <  8:339800/149‑1 (MQ=255)
                                                                                    gCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGcaa                                                                >  2:250248/1‑148 (MQ=255)
                                                                                       ttAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACaaa                                                            >  6:169070/1‑149 (MQ=255)
                                                                                             gAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCaaa                                                      <  2:315049/149‑1 (MQ=255)
                                                                                             gAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCaaa                                                      <  5:199736/149‑1 (MQ=255)
                                                                                             gAGGCGATTATCGCTGCGACGAAGCAGGGTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGc                                                                  <  4:140439/137‑1 (MQ=255)
                                                                                             gAGGCGATTATCGCTGCGACGAAGCAGGGTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGc                                                                  >  3:140439/1‑137 (MQ=255)
                                                                                                gCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCGTCTTCa                                                                                  >  8:39500/1‑118 (MQ=255)
                                                                                                gCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCCTCTTCa                                                                                  <  7:39500/118‑1 (MQ=255)
                                                                                                  gATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACaaa                                                            <  1:66669/138‑1 (MQ=255)
                                                                                                  gATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACaaa                                                            >  2:66669/1‑138 (MQ=255)
                                                                                                  gATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTgg                                                 <  2:330729/149‑1 (MQ=255)
                                                                                                   aTTATCTCTGCGACGAAGCAGGTTTAAGCAAAGAACAGGGTCGGATAAGACTTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCAATCATATTCTTCAGCGCATGAGAAACCGCAAAAAACCCAAAGGTGGc                                                <  1:271084/149‑1 (MQ=255)
                                                                                                   aTTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGc                                                <  7:194475/149‑1 (MQ=255)
                                                                                                    ttATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCg                                               <  8:139049/149‑1 (MQ=255)
                                                                                                              gacgaAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                          >  3:324527/1‑112 (MQ=255)
                                                                                                              gacgaAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                          <  4:324527/112‑1 (MQ=255)
                                                                                                              gacgaAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCg                                     >  5:297934/1‑149 (MQ=255)
                                                                                                                 gaAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTg                                  <  8:199524/149‑1 (MQ=255)
                                                                                                                  aaGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                      <  5:60244/96‑1 (MQ=255)
                                                                                                                  aaGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                      >  6:60244/1‑96 (MQ=255)
                                                                                                                      aGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCcgcg                             >  1:244339/1‑149 (MQ=255)
                                                                                                                              gCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATc                      >  1:767/1‑148 (MQ=255)
                                                                                                                              gCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATc                      >  4:346136/1‑148 (MQ=255)
                                                                                                                               cacaGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAAcc                                                                    >  2:256217/1‑101 (MQ=255)
                                                                                                                               cacaGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAAcc                                                                    <  1:256217/101‑1 (MQ=255)
                                                                                                                                acaGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATc                      >  6:38165/1‑146 (MQ=255)
                                                                                                                                  aGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGAt                 <  5:169070/149‑1 (MQ=255)
                                                                                                                                  aGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGAt                 <  2:387062/149‑1 (MQ=255)
                                                                                                                                    aaCCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATg                >  2:171194/1‑148 (MQ=255)
                                                                                                                                      tcaggTCGGATGAGACGCGAACGTCTTATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCa                                                                                  >  7:100474/4‑80 (MQ=21)
                                                                                                                                      tcaggTCGGATGAGACGCGAACGTCTTATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCa                                                                                  <  8:100474/77‑1 (MQ=21)
                                                                                                                                      ccGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGca              >  6:349487/1‑148 (MQ=255)
                                                                                                                                        gggTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACaa           >  2:187425/1‑149 (MQ=255)
                                                                                                                                         ggTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAat          >  1:239297/1‑149 (MQ=255)
                                                                                                                                         ggTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAat          <  5:38165/149‑1 (MQ=255)
                                                                                                                                              gATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAatccatc     >  4:46990/1‑149 (MQ=255)
                                                                                                                                              gATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAatccatc     >  8:102548/1‑149 (MQ=255)
                                                                                                                                                tAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCg   >  8:337851/1‑149 (MQ=255)
                                                                                                                                                 aaGACGTTCGCGTCTCATACGACCTGATTGTTTAACCCTGCCGCGCCGCTTTCGCCATCATCTTGTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCCCCATCTTTGCCGCGCCAACTCCAGATGCACACTCCATCCGc  >  2:170510/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTGCAGGCACAGTCACCACTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGC  >  NC_000913/2944860‑2945153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: