Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,945,014 G→A 5.2% intergenic (+29/+22) csdE → / ← tcdA CsdA‑binding activator; Fe‑S protein/tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,945,0140GA 5.2% 250.7 / 2.4 76intergenic (+29/+22)csdE/tcdACsdA‑binding activator; Fe‑S protein/tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA
Reads supporting (aligned to +/- strand):  ref base G (40/32);  new base A (2/2);  total (42/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CACAGTCACCACTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTCGGCCCT  >  NC_000913/2944867‑2945162
                                                                                                                                                   |                                                                                                                                                    
ctcaGTCACCTCTGTCATTGTTTTATGAGCTGTGATTACGTTCGCAGCTTAGCTCCTCACGCAGCCAGGGGTTATTTGCGTTAAGCGAGGGGATTATCGGTGCGACGAAGCAGGTTTAAGCACATAACCGGGGCGGATAAGACGTTcgc                                                                                                                                                     <  4:228967/147‑1 (MQ=255)
   aGTCACCACTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc                                                                                                                                                  <  8:75333/149‑1 (MQ=255)
        ccacTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATc                                                                                                                                             >  3:152975/1‑149 (MQ=255)
              gCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt                                                                                                                                       >  1:315049/1‑149 (MQ=255)
                   gTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGAtt                                                                                                                                   <  2:292820/148‑1 (MQ=255)
                    tttGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCAGACCTGATTGt                                                                                                                                 >  4:297706/1‑149 (MQ=255)
                      tgatgaGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGtt                                                                                                                                <  3:1617/148‑1 (MQ=255)
                           aGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAcc                                                                                                                           <  3:285386/148‑1 (MQ=255)
                             cTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCt                                                                                                                         >  1:112555/1‑148 (MQ=255)
                              tGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGa                                                                                                                       >  7:61271/1‑149 (MQ=255)
                                          cgcAGCTTAGCGCCTCACGCAGCCAGTGGTTAAATGCTTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTc                                                                                                           <  3:297706/149‑1 (MQ=255)
                                            cAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGc                                                                                                         >  8:44679/1‑149 (MQ=255)
                                               cTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATGAGACGCGAACGTCTTATCCGAccc                                                                                                                                       <  2:375403/116‑2 (MQ=38)
                                               cTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATGAGACGCGAACGTCTTATCCGAccc                                                                                                                                       >  1:375403/1‑115 (MQ=38)
                                                ttAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGa                                                                                                                                     >  3:155089/1‑117 (MQ=255)
                                                ttAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGa                                                                                                                                     <  4:155089/117‑1 (MQ=255)
                                                  aGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGTcatcat                                                                                                   >  3:62562/1‑149 (MQ=255)
                                                  aGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCcatcat                                                                                                   >  1:330729/1‑149 (MQ=255)
                                                   gcgcCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCatcatc                                                                                                  <  7:44679/149‑1 (MQ=255)
                                                      ccTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATACGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                               >  7:1200/1‑149 (MQ=255)
                                                        tCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTc                                                                                                           >  2:155188/1‑135 (MQ=255)
                                                        tCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTc                                                                                                           <  1:155188/135‑1 (MQ=255)
                                                                 cAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCAt                                                                                     >  7:199524/1‑148 (MQ=255)
                                                                  aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                               <  1:247662/137‑1 (MQ=255)
                                                                  aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                               >  2:247662/1‑137 (MQ=255)
                                                                  aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCa                                                                                           <  1:11682/141‑1 (MQ=255)
                                                                  aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCa                                                                                           >  2:11682/1‑141 (MQ=255)
                                                                  aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                                   <  1:235790/149‑1 (MQ=255)
                                                                  aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                                   <  4:152975/149‑1 (MQ=255)
                                                                  aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                                   <  2:112555/149‑1 (MQ=255)
                                                                          aaTGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCg                                                                            >  1:387062/1‑148 (MQ=255)
                                                                            tGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGcaa                                                                         <  8:339800/149‑1 (MQ=255)
                                                                             gCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGcaa                                                                         >  2:250248/1‑148 (MQ=255)
                                                                                ttAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACaaa                                                                     >  6:169070/1‑149 (MQ=255)
                                                                                      gAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCaaa                                                               <  5:199736/149‑1 (MQ=255)
                                                                                      gAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCaaa                                                               <  2:315049/149‑1 (MQ=255)
                                                                                      gAGGCGATTATCGCTGCGACGAAGCAGGGTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGc                                                                           <  4:140439/137‑1 (MQ=255)
                                                                                      gAGGCGATTATCGCTGCGACGAAGCAGGGTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGc                                                                           >  3:140439/1‑137 (MQ=255)
                                                                                         gCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCGTCTTCa                                                                                           >  8:39500/1‑118 (MQ=255)
                                                                                         gCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCCTCTTCa                                                                                           <  7:39500/118‑1 (MQ=255)
                                                                                           gATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACaaa                                                                     >  2:66669/1‑138 (MQ=255)
                                                                                           gATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACaaa                                                                     <  1:66669/138‑1 (MQ=255)
                                                                                           gATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTgg                                                          <  2:330729/149‑1 (MQ=255)
                                                                                            aTTATCTCTGCGACGAAGCAGGTTTAAGCAAAGAACAGGGTCGGATAAGACTTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCAATCATATTCTTCAGCGCATGAGAAACCGCAAAAAACCCAAAGGTGGc                                                         <  1:271084/149‑1 (MQ=255)
                                                                                            aTTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGc                                                         <  7:194475/149‑1 (MQ=255)
                                                                                             ttATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCg                                                        <  8:139049/149‑1 (MQ=255)
                                                                                                       gacgaAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                                   <  4:324527/112‑1 (MQ=255)
                                                                                                       gacgaAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                                   >  3:324527/1‑112 (MQ=255)
                                                                                                       gacgaAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCg                                              >  5:297934/1‑149 (MQ=255)
                                                                                                          gaAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTg                                           <  8:199524/149‑1 (MQ=255)
                                                                                                           aaGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                               >  6:60244/1‑96 (MQ=255)
                                                                                                           aaGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                               <  5:60244/96‑1 (MQ=255)
                                                                                                               aGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCcgcg                                      >  1:244339/1‑149 (MQ=255)
                                                                                                                       gCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATc                               >  1:767/1‑148 (MQ=255)
                                                                                                                       gCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATc                               >  4:346136/1‑148 (MQ=255)
                                                                                                                        cacaGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAAcc                                                                             >  2:256217/1‑101 (MQ=255)
                                                                                                                        cacaGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAAcc                                                                             <  1:256217/101‑1 (MQ=255)
                                                                                                                         acaGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATc                               >  6:38165/1‑146 (MQ=255)
                                                                                                                           aGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGAt                          <  5:169070/149‑1 (MQ=255)
                                                                                                                           aGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGAt                          <  2:387062/149‑1 (MQ=255)
                                                                                                                             aaCCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATg                         >  2:171194/1‑148 (MQ=255)
                                                                                                                               tcaggTCGGATGAGACGCGAACGTCTTATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCa                                                                                           <  8:100474/77‑1 (MQ=21)
                                                                                                                               tcaggTCGGATGAGACGCGAACGTCTTATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCa                                                                                           >  7:100474/4‑80 (MQ=21)
                                                                                                                               ccGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGca                       >  6:349487/1‑148 (MQ=255)
                                                                                                                                 gggTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACaa                    >  2:187425/1‑149 (MQ=255)
                                                                                                                                  ggTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAat                   <  5:38165/149‑1 (MQ=255)
                                                                                                                                  ggTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAat                   >  1:239297/1‑149 (MQ=255)
                                                                                                                                       gATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAatccatc              >  8:102548/1‑149 (MQ=255)
                                                                                                                                       gATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAatccatc              >  4:46990/1‑149 (MQ=255)
                                                                                                                                         tAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCg            >  8:337851/1‑149 (MQ=255)
                                                                                                                                          aaGACGTTCGCGTCTCATACGACCTGATTGTTTAACCCTGCCGCGCCGCTTTCGCCATCATCTTGTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCCCCATCTTTGCCGCGCCAACTCCAGATGCACACTCCATCCGc           >  2:170510/1‑149 (MQ=255)
                                                                                                                                             aCGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGtt                                            <  7:368454/113‑1 (MQ=255)
                                                                                                                                             aCGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGtt                                            >  8:368454/1‑113 (MQ=255)
                                                                                                                                             aCGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTc        >  7:190770/1‑149 (MQ=255)
                                                                                                                                             aCGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTc        <  2:767/149‑1 (MQ=255)
                                                                                                                                               gTTCGCGTCTCATCCGACCTGATAGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAAACCCAACAAACCCAAAGGTGGCGGACACCATCGTTGCCGCGCCAAATCCAGATGCACAAACCATCCGCTTCgg      >  3:211885/1‑149 (MQ=255)
                                                                                                                                                 tCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAACCCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTCGGcc    >  1:315530/1‑149 (MQ=255)
                                                                                                                                                   gcgTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTCGGCCCt  >  5:126222/1‑149 (MQ=255)
                                                                                                                                                   gcgTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTCGGCCCt  >  4:139802/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CACAGTCACCACTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTCGGCCCT  >  NC_000913/2944867‑2945162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: