Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,824,031 G→A G544G (GGG→GGA spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,824,0310GA97.5% 269.1 / ‑2.6 80G544G (GGG→GGAspoTbifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (36/42);  total (37/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTG  >  NC_000913/3823885‑3824175
                                                                                                                                                  |                                                                                                                                                
ggtAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGgaga                                                                                                                                                <  8:102191/149‑1 (MQ=255)
ggtAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGgaga                                                                                                                                                <  4:238797/149‑1 (MQ=255)
ggtAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGgaga                                                                                                                                                <  4:7086/149‑1 (MQ=255)
 gtAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGgaga                                                                                                                                                <  3:242648/148‑1 (MQ=255)
    gCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGcc                                                                                                                                            >  8:230499/1‑149 (MQ=255)
       gTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTcc                                                                                                                                         <  7:230499/149‑1 (MQ=255)
        tAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGCCGCCTCCa                                                                                                                                        >  5:168450/1‑149 (MQ=255)
          aGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCAtt                                                                                                                                      >  7:149891/1‑149 (MQ=255)
          aGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCAt                                                                                                                                       <  4:131084/148‑1 (MQ=255)
               aatgaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCa                                                                                                                                   <  2:273147/147‑1 (MQ=255)
                atgaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCg                                                                                                                                <  6:78896/149‑1 (MQ=255)
                 tgaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCgg                                                                                                                               >  6:157695/1‑149 (MQ=255)
                  gaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCgg                                                                                                                               >  7:105625/1‑148 (MQ=255)
                   aaaTCCCGCAGGAAAATTTTCAGCGCGAGCTGGATCGCATGAAGCTGGGAACGCTTTACGTTCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGGGTGGTGGTCGCTAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGc                                                                                                                              <  8:78618/148‑1 (MQ=255)
                     aTCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAAc                                                                                                                           <  3:79037/149‑1 (MQ=255)
                          gCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCaaa                                                                                                                      <  2:3312/149‑1 (MQ=255)
                          gCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCaaa                                                                                                                      <  5:157695/149‑1 (MQ=255)
                           cAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAg                                                                                                                     >  2:312276/1‑149 (MQ=255)
                              gAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCa                                                                                                                  >  8:304494/1‑149 (MQ=255)
                                    atTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACa                                                                                                            >  6:224485/1‑149 (MQ=255)
                                      tCAGCGCGAGCTGGATCGCATGAAGCTGGCAACCATTGACGATCTGCTGCCAGAAATCGGACTTCGAAACGCAATGCGCGTCGTGGTCGCGAAAAAACTGCAACATGGAGACGGCTCCATTCAACCGGCAACCCAAAGCCACGGAAATc                                                                                                          >  6:211304/1‑149 (MQ=255)
                                                   gATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAgg                                                                                             >  5:13539/1‑149 (MQ=255)
                                                       gCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGc                                                                                          <  8:105625/148‑1 (MQ=255)
                                                        cATGAAGCTGGCAACGCTTGACGATCTTCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTTGTCGCGAAAAATCTGCAACATCGAGACGCCTCCATTCCACCGGCAACCCATAGGCACGGACATCTGCCCATTAATGGTGCCg                                                                                        <  4:128874/149‑1 (MQ=255)
                                                          tGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTcc                                                                                                                                    >  1:166311/1‑103 (MQ=255)
                                                          tGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTcc                                                                                                                                    <  2:166311/103‑1 (MQ=255)
                                                          tGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGAt                                                                                      >  3:339438/1‑149 (MQ=255)
                                                           gAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGc                                                                                          >  6:127573/1‑144 (MQ=255)
                                                           gAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGc                                                                                          <  5:127573/144‑1 (MQ=255)
                                                              gCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCg                                                                                  >  8:418782/1‑149 (MQ=255)
                                                                  gCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACg                                                                                                                >  1:16243/1‑115 (MQ=255)
                                                                  gCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACg                                                                                                                <  2:16243/115‑1 (MQ=255)
                                                                    aaCGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGa                                                                            >  1:283550/1‑149 (MQ=255)
                                                                     aCGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGAt                                                                           <  5:224485/149‑1 (MQ=255)
                                                                       gCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCa                                                                         <  6:168450/149‑1 (MQ=255)
                                                                        cTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCAc                                                                        <  4:158918/149‑1 (MQ=255)
                                                                        cTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCAc                                                                        >  3:78806/1‑149 (MQ=255)
                                                                          tGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAgg                                                                                             <  8:251284/126‑1 (MQ=255)
                                                                          tGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAgg                                                                                             >  7:251284/1‑126 (MQ=255)
                                                                            aCGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCttt                                                                    <  4:339438/149‑1 (MQ=255)
                                                                              gATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGCCGCCTCCATTCCCCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGc                                                                  >  3:92192/1‑149 (MQ=255)
                                                                               aTCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATc                                                                                                          >  6:166168/1‑108 (MQ=255)
                                                                               aTCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATc                                                                                                          <  5:166168/108‑1 (MQ=255)
                                                                               aTCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAg                                                                                              <  3:40201/120‑1 (MQ=255)
                                                                               aTCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAg                                                                                              >  4:40201/1‑120 (MQ=255)
                                                                                tCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCg                                                                 >  6:404250/1‑148 (MQ=255)
                                                                                    ctgGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGaaa                                                              <  7:235978/147‑1 (MQ=255)
                                                                                    ctgGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGaaa                                                              <  7:271474/147‑1 (MQ=255)
                                                                                    ctgGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGaaa                                                              <  2:433803/147‑1 (MQ=255)
                                                                                     tgGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGccc                                                                                                     >  3:327224/1‑107 (MQ=255)
                                                                                     tgGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGccc                                                                                                     <  4:327224/107‑1 (MQ=255)
                                                                                         aGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTgcc                                                       >  2:285823/1‑149 (MQ=255)
                                                                                                ggACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTgccgc                                                     <  3:10728/144‑1 (MQ=255)
                                                                                                ggACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTgccgc                                                     >  4:10728/1‑144 (MQ=255)
                                                                                                 gACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTAtt                                               >  4:376155/1‑149 (MQ=255)
                                                                                                  aCTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACGCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTc                                              <  3:236458/149‑1 (MQ=255)
                                                                                                    ttGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAAtgctg                                                         >  3:154976/1‑136 (MQ=255)
                                                                                                    ttGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAAtgctg                                                         <  4:154976/136‑1 (MQ=255)
                                                                                                    ttGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCt                                            >  6:338434/1‑149 (MQ=255)
                                                                                                    ttGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCt                                            >  2:342749/1‑149 (MQ=255)
                                                                                                      ggTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCt                                            <  7:418782/147‑1 (MQ=255)
                                                                                                      ggTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTg                                           <  7:304494/148‑1 (MQ=255)
                                                                                                       gTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGCCGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTACCGCCCTATTCCTGGc                                         >  1:191266/1‑149 (MQ=255)
                                                                                                       gTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTgg                                          <  1:312276/148‑1 (MQ=255)
                                                                                                            gCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTg                                                                   <  5:110891/118‑1 (MQ=255)
                                                                                                            gCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTg                                                                   >  6:110891/1‑118 (MQ=255)
                                                                                                            gCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGAccc                                    >  2:55676/1‑149 (MQ=255)
                                                                                                             cAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGaaa                                                              >  3:246675/1‑122 (MQ=255)
                                                                                                             cAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGaaa                                                              <  4:246675/122‑1 (MQ=255)
                                                                                                                tGAGCGTGGTTGTAGCGAAAAATGTGCAACATGGAGACGCCTCCATTCCAGCGGCAAGCCTAAGGCACGGACAGCTGCCCATTAAAGGTGCAGATGGCGTGCTGATCACCTTTGCGAAATGCTgcc                                                       <  8:264569/126‑1 (MQ=255)
                                                                                                                tGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGAtt                                <  1:285823/149‑1 (MQ=255)
                                                                                                                tGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACAGCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTgcc                                                       >  7:264569/1‑126 (MQ=255)
                                                                                                                   gCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATc                             <  2:283550/149‑1 (MQ=255)
                                                                                                                   gCGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATc                             <  3:376155/149‑1 (MQ=255)
                                                                                                                    cGTGGTGGTCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCg                            <  6:13539/149‑1 (MQ=255)
                                                                                                                        gtggtCGCGAAAAATCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCa                        <  1:342749/149‑1 (MQ=255)
                                                                                                                                  aaacTCTGCAACATGGAGACGCCTCCATTCCACCGTCAACCCACAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATCCTACCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGcccc              <  2:191266/149‑1 (MQ=255)
                                                                                                                                  aaaaTCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAgg                                                                                             >  2:56085/1‑70 (MQ=255)
                                                                                                                                  aaaaTCTGCAACATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAgg                                                                                             <  1:56085/70‑1 (MQ=255)
                                                                                                                                            aCATGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCCCGGACATCTGCCCATTAAAGGTGCCGATGGCGGGCTGAGCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACCTCAGCCCCGGTAAAGGTc    >  6:135971/1‑149 (MQ=255)
                                                                                                                                              aTGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACCTCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTg  <  8:149891/149‑1 (MQ=255)
                                                                                                                                              aTGGAGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGGCAGCCCCGGTAAAGGTCTg  <  4:92192/149‑1 (MQ=255)
                                                                                                                                               tGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCa                   >  7:271623/1‑131 (MQ=255)
                                                                                                                                               tGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCa                   <  8:271623/131‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTG  >  NC_000913/3823885‑3824175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: