Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,213,789 T→C 5.6% Q112R (CAG→CGG)  yjaB ← GNAT‑family putative N‑acetyltransferase; acetyl coenzyme A‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,213,7890TC 5.6% 217.9 / 2.7 71Q112R (CAG→CGG) yjaBGNAT‑family putative N‑acetyltransferase; acetyl coenzyme A‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (36/31);  new base C (2/2);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

AAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCATATGCTGCCCACTTAGCAACATAAAT  >  NC_000913/4213648‑4213932
                                                                                                                                             |                                                                                                                                               
aaGTGCCGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAAGCGTCCACCTCAGAGCGTCCCGTATCCTTAAATCCCACCTTCTTATAGTACCCCACCGCCTGCTCa                                                                                                                                            <  2:253835/147‑1 (MQ=39)
 aGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCAtt                                                                                                                                          <  8:160588/148‑1 (MQ=255)
            ccGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCATCCGCCTGCTCATTTTGCTc                                                                                                                                    <  8:303714/143‑1 (MQ=255)
            ccGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCATCCGCCTGCTCATTTTGCTc                                                                                                                                    >  7:303714/1‑143 (MQ=255)
             cGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCATATTCAGAAACGGCAACGGTTTCCACAAATCGTCCACCACAGAGCGTCCCGTAACCTTACACCCCACAGTCTGAGAGAACCCCCCCGCCTGATCATTTTGCTCATTagc                                                                                                                              >  2:319659/1‑146 (MQ=255)
             cGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAAc                                                                                                                              >  8:399190/1‑148 (MQ=255)
             cGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACACACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAAc                                                                                                                              >  5:278038/1‑148 (MQ=255)
              gggATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACg                                                                                                                             <  8:134838/148‑1 (MQ=255)
                    agaAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTACCCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACgttggtt                                                                                                                       >  4:193044/1‑148 (MQ=255)
                    agaAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACgttggtt                                                                                                                       >  5:348006/1‑148 (MQ=255)
                     gaAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCAGCTCAGAGCTTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACgttggtt                                                                                                                       <  7:118830/147‑1 (MQ=255)
                      aaaaaaTCGCCTACGCGCCCACATACGCCAGATTCAGCAACGGATACGGTGTCCCCAAATCGTCCACCACAGAGCGTCCCGTACCCTTAAAACCCACCTTCTTATAGAACCCACCCGCCTGCTCATTTTGCTCATTAACgttggttg                                                                                                                      >  2:390790/1‑147 (MQ=255)
                      aaaaaaTCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTg                                                                                                                                       <  7:259866/130‑1 (MQ=255)
                      aaaaaaTCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTg                                                                                                                                       >  8:259866/1‑130 (MQ=255)
                       aaaaaTCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTc                                                                                                                    <  7:109953/148‑1 (MQ=255)
                       aaaaaTCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTc                                                                                                                    <  4:71094/148‑1 (MQ=255)
                              gCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTcc                                                                                                              <  6:348006/147‑1 (MQ=255)
                                         cacaTACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCTGTGCCATCgag                                                                                                  <  6:278038/148‑1 (MQ=255)
                                         cacaTACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATc                                                                                                     >  8:371139/1‑145 (MQ=255)
                                            aTACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAgc                                                                                               >  2:135699/1‑148 (MQ=255)
                                            aTACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAgc                                                                                               >  2:181634/1‑148 (MQ=255)
                                               cGCCAGATTCAGCAACGGATACGGTTTCCCAAACTCGTCCACCTCAGATCGGCCAGTAACCTTAAAACCCACCTTCTTTTAGAACCGAACCGCCTGCTCATTTTGCTCATTAACGTTGGGTGTCAGTTCCGGTGCCATCGAGAgcga                                                                                             <  1:319659/147‑2 (MQ=255)
                                                  cAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATg                                                                                          >  2:154088/1‑147 (MQ=255)
                                                     aTTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTc                                                                                       >  3:104525/1‑147 (MQ=255)
                                                      ttCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTcc                                                                                      >  2:320361/1‑147 (MQ=255)
                                                      ttCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTcc                                                                                      <  1:320361/147‑1 (MQ=255)
                                                          gCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAg                                                                                 >  3:259274/1‑148 (MQ=255)
                                                           cAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGc                                                                                <  1:181634/148‑1 (MQ=255)
                                                                gATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTATAACCCACATACTTATAGAACCCAACCGCCTGCTCATTTTGCTCAGTAACGTTGGTTGTCAGTTCCGGTGCCCTCGAGAGCGCATGCTCCACCAGCAccc                                                                            >  2:265943/1‑147 (MQ=255)
                                                                   aCGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGGTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGAcc                                                                        >  8:396428/1‑148 (MQ=255)
                                                                       tatCCCCAAAACGTCCACCTCAGAGCGTCCAGTAAACTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCGCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTAcg                                                                    <  4:50684/146‑1 (MQ=255)
                                                                        ttCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGa                                                                          <  7:271653/141‑1 (MQ=255)
                                                                        ttCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGa                                                                          >  8:271653/1‑141 (MQ=255)
                                                                        ttCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTAcgc                                                                   >  1:242143/1‑148 (MQ=255)
                                                                          ccccAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTAcgcc                                                                  <  7:247481/147‑1 (MQ=255)
                                                                            ccAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTcc                                                                                                              >  5:270776/1‑101 (MQ=255)
                                                                            ccAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTcc                                                                                                              <  6:270776/101‑1 (MQ=255)
                                                                              aaaTCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACgt                                                                                                                            <  2:216051/85‑1 (MQ=255)
                                                                              aaaTCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACgt                                                                                                                            >  1:216051/1‑85 (MQ=255)
                                                                               aaTCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTcc                                                                                                              <  4:124378/98‑1 (MQ=255)
                                                                               aaTCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTcc                                                                                                              >  3:124378/1‑98 (MQ=255)
                                                                                aTCGTCCACCTCAGAGCGGCCCGTCACCTTAAAACCCACCTTCTTATAGAACCCTACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGCGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCcg                                                           <  8:151736/148‑1 (MQ=255)
                                                                                        ccacAGAGCGTCCCGTAACCTTAAAACACACCTTCTTAAAGAACCCAACCGCCCGCTCATTTAGCTCATTAACGTTGGTTGTCAGTTCCCGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCAACGCCGCAGCCGCGCACAt                                                    >  1:157424/4‑147 (MQ=255)
                                                                                        ccTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCCGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACAt                                                    >  1:151856/1‑147 (MQ=255)
                                                                                            agagCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTccacc                                                                                   <  4:40090/112‑1 (MQ=255)
                                                                                            agagCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTccacc                                                                                   >  3:40090/1‑112 (MQ=255)
                                                                                            agagCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGa                                               <  1:390790/148‑1 (MQ=255)
                                                                                                    ccGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATaa                                        <  4:259274/147‑1 (MQ=255)
                                                                                                    ccGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATaa                                        <  7:371139/147‑1 (MQ=255)
                                                                                                     cGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATaaa                                       >  7:276366/1‑147 (MQ=255)
                                                                                                       tAAGCTTAAAACCCACCTTCTTATAGAACCCAACCGCCCGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACGTACGCCGCAGCCGCGCACATCAGGATCGATAAACAg                                    <  2:157424/148‑1 (MQ=255)
                                                                                                       tAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAg                                    >  7:390045/1‑148 (MQ=255)
                                                                                                       tAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCCGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAg                                    <  2:151856/148‑1 (MQ=255)
                                                                                                        aaCCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAgc                                   >  2:22279/1‑148 (MQ=255)
                                                                                                              aaaaCCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATc                              <  1:154088/147‑1 (MQ=255)
                                                                                                                aaCCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACAt                                                    >  4:300191/1‑123 (MQ=255)
                                                                                                                aaCCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACAt                                                    <  3:300191/123‑1 (MQ=255)
                                                                                                                  cccACCTTCTTGTAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCat                           >  8:189758/1‑146 (MQ=255)
                                                                                                                  cccACCTTCTTCTAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCat                           <  7:189758/146‑1 (MQ=255)
                                                                                                                   ccACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCATAtg                        >  5:85029/1‑148 (MQ=255)
                                                                                                                    cACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAg                                    <  6:63377/135‑1 (MQ=255)
                                                                                                                    cACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAg                                    >  5:63377/1‑135 (MQ=255)
                                                                                                                     aCCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCATAtgct                      >  8:14284/1‑148 (MQ=255)
                                                                                                                       cttcttATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCATAtgctgc                    <  3:193044/148‑1 (MQ=255)
                                                                                                                         tcttATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGATCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCATATGCTGcc                   >  2:218103/1‑147 (MQ=255)
                                                                                                                            tataGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGCGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCATATGCTGCCCACt               >  2:55818/1‑148 (MQ=255)
                                                                                                                               aGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCATATGCTGCCCACTTAg            >  3:369000/1‑148 (MQ=255)
                                                                                                                                gAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCATATGCTGCCCACTTAg            <  8:390045/147‑1 (MQ=255)
                                                                                                                                       aCCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCATATGCTGCCCACTTAGCAACATa     >  2:255989/1‑147 (MQ=255)
                                                                                                                                        ccGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCATATGCTGCCCACTTAGCAACATaaa   <  7:399190/148‑1 (MQ=255)
                                                                                                                                         cGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCATATGCTGCCCACTTAGCAACATAAAt  >  4:179992/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                               
AAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTGCCATCGAGAGCGCATGCTCCACCAGCACCCGACCTACGCCGCAGCCGCGCACATCAGGATCGATAAACAGCGCATCCATATGCTGCCCACTTAGCAACATAAAT  >  NC_000913/4213648‑4213932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑