Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,520,018 G→A 5.4% R103W (CGG→TGG)  yjhU ← putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,0180GA 5.4% 239.7 / 2.2 74R103W (CGG→TGG) yjhUputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element
Reads supporting (aligned to +/- strand):  ref base G (39/31);  new base A (2/2);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-01

ATAACGGTGACATTTTTACATGACTTCGGCGTCATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCA  >  NC_000913/4519876‑4520163
                                                                                                                                              |                                                                                                                                                 
ataACGGTGACATTTTTACATGACTTCGGCGTCATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCAt                                                                                                                                                >  2:42848/1‑146 (MQ=255)
 taACGGTGACATTTTTACATGACTTCGGCGTCATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAg                                                                                                                                             <  8:296980/148‑1 (MQ=255)
  aaCGGTGACATTTTTACATGACTTCGGCGTCATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACATGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGc                                                                                                                                            >  3:84869/1‑148 (MQ=255)
   aCGGTGACATTTTTACATGACTTCGGCGTCATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGc                                                                                                                                            <  6:63777/147‑1 (MQ=255)
    cGGTGACATTTTTACATGACTTCGGCGTCATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCtt                                                                                                                                          <  5:97994/148‑1 (MQ=255)
       tGACATTTTTACATGACTTCGGCGTCATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTg                                                                                                                                        >  5:346073/1‑147 (MQ=255)
           aTTTTTACATGACTTCGGCGTCATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCACATCAGCTTTGTatca                                                                                                                                   >  1:160836/1‑148 (MQ=255)
            tttttACATGACTTCGGCGTCATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGc                                                                                                                                            <  6:109814/138‑1 (MQ=255)
            tttttACATGACTTCGGCGTCATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGc                                                                                                                                            >  5:109814/1‑138 (MQ=255)
               ttACATGACTTCGGCGTCATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTAtcatca                                                                                                                                <  5:87018/147‑1 (MQ=255)
                             cGTCATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGt                                                                                                                 >  8:28109/1‑148 (MQ=255)
                                cATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCg                                                                                                              >  5:268954/1‑148 (MQ=255)
                                cATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCg                                                                                                              >  7:172074/1‑148 (MQ=255)
                                cATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCg                                                                                                              >  6:348586/1‑148 (MQ=255)
                                cATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCg                                                                                                              >  4:132278/1‑148 (MQ=255)
                                cATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCg                                                                                                              >  2:57336/1‑148 (MQ=255)
                                            cATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACg                                                                                                  <  4:9940/148‑1 (MQ=255)
                                             aTCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACg                                                                                                  <  5:348586/147‑1 (MQ=255)
                                             aTCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACg                                                                                                  <  7:286105/147‑1 (MQ=255)
                                             aTCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACg                                                                                                  <  1:319769/147‑1 (MQ=255)
                                             aTCTGATGGATTGTTCTTCCAAAGGCAACCCCCAGCACCTCCCCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCCCGTTTTCCGGCATGTTCGCCATCCGGCACga                                                                                                 >  5:115453/1‑148 (MQ=255)
                                              tCTGATGGATTGTTCTT‑CCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACgat                                                                                                >  1:31900/1‑147 (MQ=255)
                                               cTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCAt                                                                                                                   <  7:143108/128‑1 (MQ=255)
                                               cTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCAt                                                                                                                   >  8:143108/1‑128 (MQ=255)
                                                  aTGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATc                                                                                            <  3:132278/148‑1 (MQ=255)
                                                  aTGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCACATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATc                                                                                            <  2:160836/148‑1 (MQ=255)
                                                        tGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCActctc                                                                                      >  1:334868/1‑148 (MQ=255)
                                                               ccccAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGgag                                                                                 <  4:249750/146‑1 (MQ=255)
                                                                cccAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGAtt                                                                              >  2:139713/1‑148 (MQ=255)
                                                                 ccAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGAtt                                                                              >  5:1018/1‑147 (MQ=255)
                                                                  cAGGCAACCCCCAGCACATCACCATTCTCAATCCCCTGATTCAGATACATGCCACCGGCACGGGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGa                                                                            <  1:364478/148‑1 (MQ=255)
                                                                         cccccAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCTCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTTTc                                                                     >  4:287265/1‑148 (MQ=255)
                                                                                    tCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATc                                                                                            <  3:48902/114‑1 (MQ=255)
                                                                                    tCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATc                                                                                            >  4:48902/1‑114 (MQ=255)
                                                                                     ccccATTCTCAATCACCTGATTCAGATACATGCCACCGGCCAGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCAGGCATGTTCGCAATACGGCACGATGATCACTCTATGGAGATTGAATTTATCGCGAATTTACa                                                          >  6:344443/3‑147 (MQ=255)
                                                                                     cACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCACATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCa                                                          >  7:261644/1‑147 (MQ=255)
                                                                                       ccATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAag                                                       >  8:259990/1‑148 (MQ=255)
                                                                                       ccATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAag                                                       >  6:226236/1‑148 (MQ=255)
                                                                                        cATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTAAGCATCAGCTTTGTATCATCACGTTATCCGGCATGTTCCCCATCCGGCACGATGATCACTCTATGGAGATTGAATTTATCGCGAATTTGCAAag                                                       >  3:205343/1‑147 (MQ=255)
                                                                                          ttCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGc                                                                                                                     >  6:271650/1‑83 (MQ=255)
                                                                                          ttCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGc                                                                                                                     <  5:271650/83‑1 (MQ=255)
                                                                                          ttCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCATTGTATCATCACGTTTTCCGGCATGTTCGCCATCAGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAagcag                                                    >  4:210046/1‑148 (MQ=255)
                                                                                           tctcAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCa                                                                                                    >  5:331162/1‑99 (MQ=255)
                                                                                           tctcAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCa                                                                                                    <  6:331162/99‑1 (MQ=255)
                                                                                              cAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTc                                                >  5:76192/1‑148 (MQ=255)
                                                                                               aaTCACCTGATTCAGATACATGCCACCGGCACGTGAAATGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCTCGAATTTGCAAAGCAGCg                                                  <  2:334868/145‑1 (MQ=255)
                                                                                               aaTCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCg                                               <  6:346073/148‑1 (MQ=255)
                                                                                                  cACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGAGAATTTGCAAAGCAGCGTCGa                                              >  2:246218/1‑146 (MQ=255)
                                                                                                  cACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATAACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGa                                              >  8:306600/1‑146 (MQ=255)
                                                                                                   aCCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTa                                                                       >  1:335360/1‑120 (MQ=255)
                                                                                                   aCCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTa                                                                       <  2:335360/120‑1 (MQ=255)
                                                                                                   aCCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATa                                            <  1:57336/147‑1 (MQ=255)
                                                                                                      tGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGTATATTGAATTTATCGCGAATTTGCAAAGCAGATTCGATatag                                         >  2:164722/1‑147 (MQ=255)
                                                                                                       gATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGc                                        >  5:285291/1‑147 (MQ=255)
                                                                                                        aTTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGCTGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGCTAGAGcc                                       >  2:274776/1‑147 (MQ=255)
                                                                                                        aTTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGcc                                       >  6:123728/1‑147 (MQ=255)
                                                                                                         ttCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATAACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCaa                                     <  7:306600/148‑1 (MQ=255)
                                                                                                         ttCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATCTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCa                                      >  1:110426/1‑147 (MQ=255)
                                                                                                               tACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACAt                               <  8:172074/148‑1 (MQ=255)
                                                                                                                  aTGCCACCGGCACGTGAAAGGCGAGTCCACATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTca                            <  8:261644/148‑1 (MQ=255)
                                                                                                                     ccACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGa                                                                            <  2:375475/97‑1 (MQ=255)
                                                                                                                     ccACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGa                                                                            >  1:375475/1‑97 (MQ=255)
                                                                                                                      cACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGc                                                           <  4:136578/113‑1 (MQ=255)
                                                                                                                      cACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGc                                                           >  3:136578/1‑113 (MQ=255)
                                                                                                                      cACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATc                        <  1:42848/148‑1 (MQ=255)
                                                                                                                       aCCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGGTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATcc                       <  1:246218/148‑1 (MQ=255)
                                                                                                                        ccGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGAt                                             <  7:222305/125‑1 (MQ=255)
                                                                                                                        ccGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGAt                                             >  8:222305/1‑125 (MQ=255)
                                                                                                                        ccGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACAtt                              >  8:157191/1‑140 (MQ=255)
                                                                                                                        ccGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACAtt                              <  7:157191/140‑1 (MQ=255)
                                                                                                                              cGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGa                <  7:28109/148‑1 (MQ=255)
                                                                                                                              cGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGa                <  7:259990/148‑1 (MQ=255)
                                                                                                                                      gCGAGTCCGCATCAGCTTTGTATCATCACGTTTTACGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAAcc        <  6:268954/148‑1 (MQ=255)
                                                                                                                                            ccGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCa  >  3:191006/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
ATAACGGTGACATTTTTACATGACTTCGGCGTCATGGTTTTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCA  >  NC_000913/4519876‑4520163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑