Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 724,846 T→A 6.2% E45V (GAA→GTA)  kdpC ← potassium translocating ATPase, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913724,8460TA 6.2% 206.1 / 3.1 64E45V (GAA→GTA) kdpCpotassium translocating ATPase, subunit C
Reads supporting (aligned to +/- strand):  ref base T (30/30);  new base A (2/2);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

AGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACC  >  NC_000913/724704‑724987
                                                                                                                                              |                                                                                                                                             
aGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACg                                                                                                                                           <  5:199363/147‑1 (MQ=255)
 gggTTACTGCCCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGaa                                                                                                                                         >  2:183223/1‑148 (MQ=255)
    ttACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCa                                                                                                                                      <  8:329953/148‑1 (MQ=255)
    ttACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCa                                                                                                                                      <  3:13138/148‑1 (MQ=255)
       cTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACg                                                                                                                                   <  1:365434/148‑1 (MQ=255)
          aCCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGa                                                                                                                                  <  7:246034/146‑1 (MQ=255)
             gCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCAt                                                                                                                             >  3:179391/1‑148 (MQ=255)
                 gATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGaa                                                                                                                                         >  4:364097/1‑132 (MQ=255)
                 gATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGaa                                                                                                                                         <  3:364097/132‑1 (MQ=255)
                    tGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTg                                                                                                                      >  1:52105/1‑148 (MQ=255)
                     gCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTg                                                                                                                      <  4:44512/147‑1 (MQ=255)
                          cGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCCTTGGCCTGCCAgg                                                                                                                 >  2:101749/1‑147 (MQ=255)
                                aaGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTg                                                                                                                      <  1:238840/136‑1 (MQ=255)
                                aaGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTg                                                                                                                      >  2:238840/1‑136 (MQ=255)
                                   ccTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAAcctcca                                                                                                       <  7:399976/148‑1 (MQ=255)
                                   ccTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGGTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAAccacca                                                                                                       <  5:34845/148‑1 (MQ=255)
                                     tgtgGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCAtt                                                                                                     >  3:296194/1‑148 (MQ=255)
                                      gtgGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTg                                                                                                    >  4:146508/1‑148 (MQ=255)
                                       tgGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAg                                                                                                                  >  6:217686/1‑133 (MQ=255)
                                       tgGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAg                                                                                                                  <  5:217686/133‑1 (MQ=255)
                                       tgGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCAtt                                                                                                     <  1:190164/146‑1 (MQ=255)
                                                   cATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCCCCTTCACGAATCCACGCACCATTGGCCTGCCAGGGAAGCCACCATTGCCCCAGTACggtg                                                                                       >  5:83945/1‑148 (MQ=255)
                                                      ttCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACggtggt                                                                                     >  2:203767/1‑147 (MQ=255)
                                                           ccGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTcagcag                                                                               <  6:273330/148‑1 (MQ=255)
                                                               tGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCggg                                                                           >  5:79830/1‑148 (MQ=255)
                                                                  cGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTaa                                                                        <  3:146508/148‑1 (MQ=255)
                                                                  cGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGGCAGCAGCGGGTaa                                                                        <  1:183223/148‑1 (MQ=255)
                                                                  cGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCATCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTaa                                                                        <  3:137747/148‑1 (MQ=255)
                                                                    aCGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAAc                                                                      >  2:163266/1‑148 (MQ=255)
                                                                    aCGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAAc                                                                      >  2:356319/1‑148 (MQ=255)
                                                                      gggCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAAcgc                                                                    <  2:52105/148‑1 (MQ=255)
                                                                           aCCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAAcgccgc                                                                 >  2:270925/1‑146 (MQ=255)
                                                                             cATGATAATAGCCTTTGCCGTTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCTCGAATCa                                                                                                                                      <  8:346410/75‑1 (MQ=255)
                                                                              aTGAAAATAGCCGTTGCAGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATaaa                                                                                                                                     >  7:346410/1‑75 (MQ=255)
                                                                                gAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTACACGAGTCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTaa                                                                        <  6:216112/134‑1 (MQ=255)
                                                                                gAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTACACGAGTCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTaa                                                                        >  5:216112/1‑134 (MQ=255)
                                                                                      aGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATaa                                                    >  1:278428/1‑148 (MQ=255)
                                                                                            tGCCGGTAAAATTCTGCCCGATTAATGCCGACCCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCCGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGa                                                >  1:384748/1‑146 (MQ=255)
                                                                                            tGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGa                                                >  1:275462/1‑146 (MQ=255)
                                                                                                ggTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGAt                                           >  1:91074/1‑147 (MQ=255)
                                                                                                ggTAAAATTCTGCCCGATTAATGACGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGAt                                           >  1:85676/1‑147 (MQ=255)
                                                                                                  tAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATaaa                                        >  5:360945/1‑148 (MQ=255)
                                                                                                    aaaTTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATg                                      <  6:360945/148‑1 (MQ=255)
                                                                                                        tCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGa                                  >  3:170696/1‑148 (MQ=255)
                                                                                                         cTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGa                                  <  4:296194/147‑1 (MQ=255)
                                                                                                           gCCCCATTAATCCCGAACAGCGCACCGTATCACCATCACGAATCAACGAACAAATAGCCTGCCAGGGAAACCACCATTGCCCCAGAACGGTGGTCAGCAGCCGGTAAACGCCTCCAGAAATCAATAACAGAAACAAAAAAGATGATaa                               >  8:372231/1‑148 (MQ=255)
                                                                                                            cccGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATa                                <  8:22898/146‑1 (MQ=255)
                                                                                                            cccGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATa                                <  1:270925/146‑1 (MQ=255)
                                                                                                            cccGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAAt                              >  6:237971/1‑148 (MQ=255)
                                                                                                                aTTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTG‑CCCAGTACGGTGGTCAGCAGCGGGTAAACgccgcc                                                                >  1:396083/1‑109 (MQ=255)
                                                                                                                aTTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTG‑CCCAGTACGGTGGTCAGCAGCGGGTAAACgccgcc                                                                <  2:396083/109‑1 (MQ=255)
                                                                                                                      gCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACgccgcc                                                                >  7:378042/1‑104 (MQ=255)
                                                                                                                      gCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACgccgcc                                                                <  8:378042/104‑1 (MQ=255)
                                                                                                                      gCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATa                                >  4:57167/1‑136 (MQ=255)
                                                                                                                      gCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATa                                <  3:57167/136‑1 (MQ=255)
                                                                                                                        cGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAAt                  <  2:46980/148‑1 (MQ=255)
                                                                                                                        cGAACCGCGCACCGTATCACCTACACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAAt                  >  2:23532/1‑148 (MQ=255)
                                                                                                                           aCCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAATCCa               <  5:237971/148‑1 (MQ=255)
                                                                                                                            ccGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAATCCAc              <  2:275462/148‑1 (MQ=255)
                                                                                                                             cgcgCACCGTATCACCTACACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAATCCACt             <  1:23532/148‑1 (MQ=255)
                                                                                                                                   ccGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATgg        >  4:55219/1‑147 (MQ=255)
                                                                                                                                     gTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTaa     <  6:79830/148‑1 (MQ=255)
                                                                                                                                       aTCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAAc   >  5:347320/1‑148 (MQ=255)
                                                                                                                                        tCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCCCCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAAcc  <  6:385981/148‑1 (MQ=255)
                                                                                                                                             ttCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCAt          <  7:20086/135‑1 (MQ=255)
                                                                                                                                             ttCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCAt          >  8:20086/1‑135 (MQ=255)
                                                                                                                                              |                                                                                                                                             
AGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACC  >  NC_000913/724704‑724987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑