Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 814,856 C→T 9.1% A444V (GCA→GTA)  uvrB → exision nuclease of nucleotide excision repair, DNA damage recognition component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913814,8560CT 9.1% 129.2 / 3.4 44A444V (GCA→GTA) uvrBexision nuclease of nucleotide excision repair, DNA damage recognition component
Reads supporting (aligned to +/- strand):  ref base C (20/20);  new base T (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

CGCCGGGTAATTACGAGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGA  >  NC_000913/814709‑814997
                                                                                                                                                   |                                                                                                                                             
cGCCGGGTAATTACGAGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGc                                                                                                                                               <  3:62540/148‑1 (MQ=255)
     ggTAATTACGAGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAAtt                                                                                                                                           >  5:184093/1‑147 (MQ=255)
     ggTAATTACGAGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTa                                                                                                                                          <  3:144045/148‑1 (MQ=255)
          ttACGAGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTaacgaa                                                                                                                                     >  1:304606/1‑148 (MQ=255)
           tACGAGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTaacgaac                                                                                                                                    >  4:67711/1‑148 (MQ=255)
           tACGAGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTaacgaac                                                                                                                                    >  2:402064/1‑148 (MQ=255)
            aCGAGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTaacgaacg                                                                                                                                   >  1:233036/1‑148 (MQ=255)
             cGAGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAAcgc                                                                                                                                  >  2:164140/1‑148 (MQ=255)
               aGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAAcgcg                                                                                                                                 >  4:120574/1‑147 (MQ=255)
               aGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGt                                                                                                                                <  3:120574/148‑1 (MQ=255)
                gCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTCACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTa                                                                                                                               <  2:211193/148‑1 (MQ=255)
                     aaaaaTCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGCACGCGTACTGGt                                                                                                                          >  4:293621/1‑148 (MQ=255)
                         aTCCTGCGGCGATTTGGTGGATCTGGTTGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGAGGCCGTTGGCGACACAGGTTGATTATATTCTTTCTTATATTCGTCAGGGAGCGGCAATTAACGATCGCGTACTGGTcacc                                                                                                                      <  6:184093/148‑1 (MQ=255)
                          tCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGAGGCGACACAGGTTGATGAGCTTCTTTCGGAGATTCGTCAGCGAGCGACAATTAACGAACGCGTACTGGTcacca                                                                                                                     >  3:234916/1‑148 (MQ=255)
                            cggcggCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTcaccac                                                                                                                    <  6:132741/147‑1 (MQ=255)
                               cggcgATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTg                                                                                                                <  2:304606/148‑1 (MQ=255)
                                  cgATGTGGTGGATCAGGTGGTGCGTCCAACCGTATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACAACACTGAc                                                                                                              >  4:360112/1‑147 (MQ=255)
                                   gATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGTAATTAACGAACGCGTACTGGTCACCACACTGAcc                                                                                                             >  1:208741/1‑147 (MQ=255)
                                         gtggATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCgg                                                                                                       <  1:164140/147‑1 (MQ=255)
                                           ggATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATg                                                                                                    <  3:357491/148‑1 (MQ=255)
                                                 ggtggtGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGaa                                                                                              <  2:38013/148‑1 (MQ=255)
                                                     gtgCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGTAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGAt                                                                                           <  2:208741/147‑1 (MQ=255)
                                                           ccAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTAcc                                                                                     >  7:101595/1‑147 (MQ=255)
                                                                      gCTTGACCCGATTATCGAATTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCga                                                                          <  3:67711/147‑1 (MQ=255)
                                                                      gCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCga                                                                          <  8:101595/147‑1 (MQ=255)
                                                                          gACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCgaagaa                                                                      <  2:45397/147‑1 (MQ=255)
                                                                           aCCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAAc                                                                     <  8:77340/147‑1 (MQ=255)
                                                                               gATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGCCCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGc                                                                >  5:215453/1‑148 (MQ=255)
                                                                                      gAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGTAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGcgcg                                                         >  8:86801/1‑148 (MQ=255)
                                                                                       aaGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGTAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCgcgt                                                        <  7:86801/148‑1 (MQ=255)
                                                                                          tGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCgcgtgcg                                                     >  8:406929/1‑148 (MQ=255)
                                                                                             ggCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCTAGCGGCAATTAACGAACGAGTACTTGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTa                                                  <  7:346805/148‑1 (MQ=255)
                                                                                                cGGTGGCGACACAGGTTGATGATCTTCTTTCGGATATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCt                                               <  1:392961/148‑1 (MQ=255)
                                                                                                             ggTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATc                                  >  7:103773/1‑148 (MQ=255)
                                                                                                               ttGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGa                                >  3:272042/1‑148 (MQ=255)
                                                                                                                      tcttctTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTc                         >  4:137024/1‑148 (MQ=255)
                                                                                                                      tcttctTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGAGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCg                           >  5:59639/1‑146 (MQ=255)
                                                                                                                      tcttctTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGAGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCg                           <  6:59639/146‑1 (MQ=255)
                                                                                                                          gtttCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGt                          <  4:272042/142‑1 (MQ=255)
                                                                                                                                   aTTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATg                >  4:220503/1‑144 (MQ=255)
                                                                                                                                        tCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATc       >  1:97709/1‑148 (MQ=255)
                                                                                                                                         cAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGa                                                                                   <  7:100673/71‑1 (MQ=255)
                                                                                                                                         cAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGa                                                                                   >  8:100673/1‑71 (MQ=255)
                                                                                                                                          agcgagcgGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCcg     <  1:402064/148‑1 (MQ=255)
                                                                                                                                             gagcgGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGa  <  7:406929/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CGCCGGGTAATTACGAGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGA  >  NC_000913/814709‑814997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑