Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,349,850	C→T	6.8%	L86L (TTG→TTA)	yciW ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,349,850	0	C	T	6.8%	188.8 / 2.4	59	L86L (TTG→TTA)	yciW	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (22/33);  new base T (2/2);  total (24/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGAAAGGG  >  NC_000913/1349704‑1349996                                                                                                                                                   |                                                                                                                                                   aGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACa                                                                                                                                                   <  6:273999/148‑1 (MQ=255) aGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACa                                                                                                                                                   <  1:34392/148‑1 (MQ=255) aGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCCCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACa                                                                                                                                                   <  7:16891/148‑1 (MQ=255)   ttGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCC‑GGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGACAGGGCCGTCGACAAGc                                                                                                                                                >  3:287605/1‑148 (MQ=255)       cGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGtt                                                                                                                                             <  1:82684/148‑1 (MQ=255)       cGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGtt                                                                                                                                             >  2:397202/1‑148 (MQ=255)        gggCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTc                                                                                                                                            <  4:312428/148‑1 (MQ=255)              atTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGATAAGCGTTCATAAGc                                                                                                                                      >  4:182939/1‑148 (MQ=255)              atTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGATAAGCGTTCATAAGc                                                                                                                                      >  4:177124/1‑148 (MQ=255)              atTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATa                                                                                                                                         >  1:70262/1‑145 (MQ=255)              atTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATa                                                                                                                                         <  2:70262/145‑1 (MQ=255)              atTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGc                                                                                                                                      >  3:109825/1‑148 (MQ=255)               ttGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGc                                                                                                                                      <  4:116437/147‑1 (MQ=255)               ttGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCg                                                                                                                                     <  3:189274/148‑1 (MQ=255)                  cGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGt                                                                                                                                   <  6:380489/147‑1 (MQ=255)                   gTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTg                                                                                                                                  >  3:343140/1‑147 (MQ=255)                      aTTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAgg                                                                                                                              <  3:187221/148‑1 (MQ=255)                      aTTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCACGGCCGTCGACAAGCGTTCATAAGCGGTGAAgg                                                                                                                              <  1:191739/148‑1 (MQ=255)                           tgCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCtg                                                                                                                          <  1:104646/147‑1 (MQ=255)                           tgCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCtg                                                                                                                          <  4:69422/147‑1 (MQ=255)                           tgCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGGCtg                                                                                                                          <  1:397202/147‑1 (MQ=255)                             ccAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGATAAGCGTTCATAAGCGGTGAAGGTCTGTGa                                                                                                                       <  3:177124/148‑1 (MQ=255)                             ccAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGATAAGCGTTCATAAGCGGTGAAGGTCTGTGa                                                                                                                       <  3:182939/148‑1 (MQ=255)                                aCCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACg                                                                                                                    <  8:351947/148‑1 (MQ=255)                                 ccGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACgg                                                                                                                   >  6:304812/1‑148 (MQ=255)                                              ttccTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGTGGTGAAGGTCTGTGAACGGGTCACGGTAAg                                                                                                        <  1:146294/144‑1 (MQ=255)                                                   ggtggAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTTGACGACTCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCCCGGTAAGCTGGTCa                                                                                                 <  2:60602/148‑1 (MQ=255)                                                    gtggAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGTGTCACGGTAAGCTGGGCAg                                                                                                <  2:117261/148‑1 (MQ=255)                                                    gtggAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCACAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTc                                                                                                  <  8:277093/146‑1 (MQ=255)                                                    gtggAATCAGGCCC‑GGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTGATAAGCGGTGAAGGTCTGTGAACGTGTCACGGTATGTTGGTTAg                                                                                                <  4:287605/147‑1 (MQ=255)                                                     tggAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGACAgg                                                                                               >  4:252185/1‑148 (MQ=255)                                                        aaTCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGaa                                                                                                                                <  3:342826/112‑1 (MQ=255)                                                        aaTCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGaa                                                                                                                                >  4:342826/1‑112 (MQ=255)                                                            aGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACaaa                                                                                        <  3:252185/148‑1 (MQ=255)                                                                  ggggAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGCGTCACGGTAAGCTGGTCAGGGAACAAATCCACt                                                                                  >  8:26210/1‑148 (MQ=255)                                                                         ggcgTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGaa                                                                           <  4:343140/148‑1 (MQ=255)                                                                             tAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCa                                                                                                 >  2:358885/1‑122 (MQ=255)                                                                             tAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCa                                                                                                 <  1:358885/122‑1 (MQ=255)                                                                              aaGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGACCAcgc                                                                      >  2:368555/1‑148 (MQ=255)                                                                              aaGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACAcgc                                                                      >  8:52702/1‑148 (MQ=255)                                                                              aaGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACAcgc                                                                      >  4:50775/1‑148 (MQ=255)                                                                                 tCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACAcgcg                                                                     >  1:212173/1‑146 (MQ=255)                                                                                   gcgcCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAgg                                                                 <  2:212173/148‑1 (MQ=255)                                                                                                gtTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGaa                                                    <  5:304812/148‑1 (MQ=255)                                                                                                    cATAACCGCTGGACGCCTCAAACATTAGCAACCGTCAGGGCCGTCGACAAGCTTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTTGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAaagga                                                <  4:109825/148‑1 (MQ=255)                                                                                                    cATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTTTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAaagg                                                 <  6:278097/147‑1 (MQ=255)                                                                                                    cATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCAc                                                                                                              <  3:334498/86‑1 (MQ=255)                                                                                                    cATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCAc                                                                                                              >  4:334498/1‑86 (MQ=255)                                                                                                                 cgccgcAAACCTGCGCTACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAaagga                                                <  8:205987/135‑1 (MQ=255)                                                                                                                  gccgcAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGCTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACCCGCGCAGGGTACAGCCATGAaaggaa                                               >  7:205987/1‑135 (MQ=255)                                                                                                                          ccTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCttttt                           >  7:387897/1‑147 (MQ=255)                                                                                                                          ccTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGATCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCtattt                           >  2:394727/1‑147 (MQ=255)                                                                                                                            tGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCaa                        >  2:323406/1‑148 (MQ=255)                                                                                                                                  aCCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAaagga                                                >  7:18108/1‑118 (MQ=255)                                                                                                                                  aCCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAaagga                                                <  8:18108/118‑1 (MQ=255)                                                                                                                                     gTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGt               <  1:323406/148‑1 (MQ=255)                                                                                                                                       cAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTc              >  8:332740/1‑147 (MQ=255)                                                                                                                                                  aCAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGAAAgg   >  4:104011/1‑147 (MQ=255)                                                                                                                                                  aCAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGATCAGGGAACAAATCCACTGCAATAACACGCGCATGGTCCAGCAATGTAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGAAAggg  <  1:394727/148‑1 (MQ=255)                                                                                                                                                   cAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGaaa     >  7:149666/1‑144 (MQ=255)                                                                                                                                                   cAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGaaa     <  8:149666/144‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   AGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGAAAGGG  >  NC_000913/1349704‑1349996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑