Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,525,378 G→A 9.1% N201N (AAC→AAT)  ansP ← L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,525,3780GA 9.1% 131.0 / 3.4 44N201N (AAC→AAT) ansPL‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base G (19/21);  new base A (2/2);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

TACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTA  >  NC_000913/1525242‑1525525
                                                                                                                                        |                                                                                                                                                   
tACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCa                                                                                                                                               <  6:154933/143‑1 (MQ=255)
      ctcCGTCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCt                                                                                                                                     <  5:391818/147‑1 (MQ=255)
                  tACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGTAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCg                                                                                                                          <  2:183643/146‑1 (MQ=255)
                  tACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTTATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCg                                                                                                                          <  7:131400/146‑1 (MQ=255)
                  tACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAg                                                                                                                        <  8:55298/148‑1 (MQ=255)
                      caccaTTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGaa                                                                                                                     >  8:87090/1‑147 (MQ=255)
                           tttCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGt                                                                                                               <  8:394257/148‑1 (MQ=255)
                            ttCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTATTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTa                                                                                                              >  3:175912/1‑148 (MQ=255)
                            ttCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACAGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTATTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTa                                                                                                              >  3:181715/1‑148 (MQ=255)
                               aaTGGAGGCAAAAGCAAACACTACGCCCTGAATCTACACCAGCGCAGGCAGCAACCCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTAccc                                                                                                           <  8:194175/148‑1 (MQ=255)
                                         aaaGCAAACACTCCGCCCTGAATCAACACCAGCGCAGGCAGCACACCGAGGGGGAAGACGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCaaa                                                                                                  >  1:348412/1‑147 (MQ=255)
                                         aaaGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAAGCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGCCTGACAACTACCGAGGAACACTCTACCCACGACCaaa                                                                                                  >  1:354012/1‑147 (MQ=255)
                                         aaaGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCaaa                                                                                                  >  7:51817/1‑147 (MQ=255)
                                         aaaGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCaaa                                                                                                  >  6:59261/1‑147 (MQ=255)
                                              aaaCACTACGCCCTGAATCAACACCAGAGCAAGCAGCAAACCGTGGGGGAAGAAGCCGCAATTATCGGACATTAAATGAAAGCCAGAGGTGATGCCATCCAGCGGCTCACCACTCCCGAGGAACACTGTACCCACCACCAAAAAggg                                                                                             >  6:201621/1‑146 (MQ=255)
                                                         ccTGAATCAACAACAGCGCAGGCAGCAAACCGTGGGGGAAGAATCCGCCATTATCGGTGATTAAATGAAAGCCAGTTGGGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAg                                                                                 <  2:207336/148‑1 (MQ=255)
                                                          cTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAg                                                                                 >  8:6096/1‑147 (MQ=255)
                                                          cTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGc                                                                                <  7:87090/148‑1 (MQ=255)
                                                            gAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGa                                                                                  <  5:122043/144‑1 (MQ=255)
                                                            gAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGa                                                                                  >  6:122043/1‑144 (MQ=255)
                                                            gAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCAc                                                                              >  8:104492/1‑148 (MQ=255)
                                                             aaTCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTATTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACt                                                                             <  4:181715/148‑1 (MQ=255)
                                                              aTCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTATTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACt                                                                             <  4:175912/147‑1 (MQ=255)
                                                               tCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACttt                                                                           <  3:253744/148‑1 (MQ=255)
                                                                  acacCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTtaa                                                                         >  7:392607/1‑147 (MQ=255)
                                                                      cAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAgc                                                                    >  4:153027/1‑148 (MQ=255)
                                                                           caggcagCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCaaa                                                               >  3:71490/1‑148 (MQ=255)
                                                                              gcagcaAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCa                                                            <  7:6096/148‑1 (MQ=255)
                                                                                agcaAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGa                                                          <  8:392607/148‑1 (MQ=255)
                                                                                    aaCCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTc                                                      >  5:230973/1‑148 (MQ=255)
                                                                                     aCCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTcc                                                     <  3:153027/148‑1 (MQ=255)
                                                                                                   agCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCAc                                                                              >  4:122299/1‑109 (MQ=255)
                                                                                                   agCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCAc                                                                              <  3:122299/109‑1 (MQ=255)
                                                                                                    gccgccATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCCGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGACCTCCATCTCCGCAAACCAt                                      >  1:142101/1‑148 (MQ=255)
                                                                                                      cgccATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCAttt                                    >  8:126996/1‑148 (MQ=255)
                                                                                                            tATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACAcc                              >  2:401186/1‑148 (MQ=255)
                                                                                                              tCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGa                            <  8:51817/148‑1 (MQ=255)
                                                                                                                  tGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCat                        <  5:201621/148‑1 (MQ=255)
                                                                                                                       aaaTGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCa                   >  5:188024/1‑148 (MQ=255)
                                                                                                                        aaTGATAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCAt                  <  4:71490/148‑1 (MQ=255)
                                                                                                                        aaTGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCAt                  <  6:188024/148‑1 (MQ=255)
                                                                                                                           gAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCAt                  >  7:190981/1‑145 (MQ=255)
                                                                                                                           gAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCAt                  <  8:190981/145‑1 (MQ=255)
                                                                                                                            aacgCCAGTGGGGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTg              <  5:59261/145‑1 (MQ=255)
                                                                                                                                     ggtgTTGCCTTCCAGGGGCTGACCACTACCGATGATCTCTGTACCGACTACCATAAAGGTCACTATGGCGACGACTTTAATAAGCGCAAACCAGAACTCCATGTCGGCAATCGATTTCAAACCGATCATATTCATGGTGACAACCATg     <  2:348412/148‑1 (MQ=255)
                                                                                                                                     ggtgTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCg                                                                                   >  5:379686/1‑70 (MQ=255)
                                                                                                                                     ggtgTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCg                                                                                   <  6:379686/70‑1 (MQ=255)
                                                                                                                                     ggtgTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATg     >  1:161949/1‑148 (MQ=255)
                                                                                                                                      gtgtTGCCATCCATCGGCTGACCACTAGCGATGAACACTGTACCCATGACCAAAAAGGTCACATTTGGTAGCTCTTTAATAAGAGAAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCATCGagg     <  2:354012/147‑3 (MQ=255)
                                                                                                                                        gtTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCTGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTa  <  2:142101/148‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                   
TACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTA  >  NC_000913/1525242‑1525525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑