Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,439,269 G→C 6.2% V284V (GTC→GTG yfcJ ← putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,439,2690GC 6.2% 215.5 / 2.1 63V284V (GTC→GTGyfcJputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (30/29);  new base C (2/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGCGAAACGAAAGTCCCG  >  NC_000913/2439124‑2439416
                                                                                                                                                 |                                                                                                                                                   
aGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGAc                                                                                                                                                   <  2:303435/148‑1 (MQ=255)
 ggggACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGAc                                                                                                                                                   >  8:222767/1‑147 (MQ=255)
  gtgACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGAGACCCATGCACCTGGGGCTTGCCAGAGCCGCAACAATCCCACCGTTTCTACAAGCAGAGAGACaa                                                                                                                                                 >  2:224738/3‑148 (MQ=255)
     aCGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCg                                                                                                                                              <  6:376870/148‑1 (MQ=255)
          tttAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACCATCGCCACt                                                                                                                                         >  5:345073/1‑148 (MQ=255)
              accaccTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTc                                                                                                                                      <  1:199639/147‑1 (MQ=255)
              accaccTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCa                                                                                                                                     <  2:117951/148‑1 (MQ=255)
              accaccTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCCACAAGCAGAGAGACAATCGCCACTTTc                                                                                                                                      <  5:61247/147‑1 (MQ=255)
                   cTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCAcgccg                                                                                                                                >  8:393623/1‑148 (MQ=255)
                      cACGCCCAGCGCCGGAAAGATAAGCGAACAGCCGGCGCAGGTTAACGCCGCGCCCGCTAATGCGACCAAGGCAGCTGGGGCTTGCCAGAGCATCAACAAGCCCTCCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCa                                                                                                                             <  6:91616/148‑1 (MQ=255)
                            cAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGACACAATCGCCACTTTCACGCCGCCAAAACgg                                                                                                                       >  4:114707/1‑148 (MQ=255)
                            cAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGACACAATCGCCACTTTCACGCCGCCAAAACg                                                                                                                        >  4:109540/1‑147 (MQ=255)
                                  aGGAAATATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCACGCTAATGCGAGCCTTGAACCTGGGGCTTGCCAGAGGAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAAGCGCCACTTTCACGCCGCCAAACCGGTCCGGc                                                                                                                 <  1:224738/148‑1 (MQ=255)
                                   ggAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCa                                                                                                                >  2:353088/1‑148 (MQ=255)
                                   ggAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCa                                                                                                                <  4:173713/148‑1 (MQ=255)
                                   ggAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCa                                                                                                                >  3:233001/1‑148 (MQ=255)
                                     aaaGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTc                                                                                                                                      >  5:290263/1‑124 (MQ=255)
                                     aaaGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTc                                                                                                                                      <  6:290263/124‑1 (MQ=255)
                                                      cGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGAcgcg                                                                                             <  6:147021/148‑1 (MQ=255)
                                                          tCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCAt                                                                                          >  1:285652/1‑147 (MQ=255)
                                                           ccGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCa                                                                                        >  5:172322/1‑148 (MQ=255)
                                                            cGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGACCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATc                                                                                         <  5:239659/146‑1 (MQ=255)
                                                             ggTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCAc                                                                                       >  8:64482/1‑147 (MQ=255)
                                                              gTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAATGGTCCGGCATCCAGCCAAACATGACGCGCATCACGa                                                                                     <  5:191599/148‑1 (MQ=255)
                                                              gTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGa                                                                                     <  7:393623/148‑1 (MQ=255)
                                                              gTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCACCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGa                                                                                     <  3:29211/148‑1 (MQ=255)
                                                                   cgccgcGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAAt                                                                                >  7:75339/1‑148 (MQ=255)
                                                                            cGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCgccgcc                                                                        >  1:187000/1‑147 (MQ=255)
                                                                                 aTGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGACACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAAcgc                                                                  <  3:114707/148‑1 (MQ=255)
                                                                                 aTGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGACACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAAcgc                                                                  <  3:109540/148‑1 (MQ=255)
                                                                                          aTGCACCTGGGGTTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAagag                                                          >  2:196832/1‑147 (MQ=255)
                                                                                          aTGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAagag                                                          >  7:34432/1‑147 (MQ=255)
                                                                                            gCACCTGGGTCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCaa                                                                      >  4:55829/1‑133 (MQ=255)
                                                                                            gCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTa                                                        >  5:381823/1‑147 (MQ=255)
                                                                                            gCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGGTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCaa                                                                      <  3:55829/133‑1 (MQ=255)
                                                                                             cACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTaa                                                       <  1:78575/147‑1 (MQ=255)
                                                                                             cACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTaa                                                       <  2:187000/147‑1 (MQ=255)
                                                                                              aCCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAg                                                     <  4:368005/148‑1 (MQ=255)
                                                                                                 tGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGccc                                                  >  1:217536/1‑148 (MQ=255)
                                                                                                  ggggCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCg                                                 >  2:236819/1‑148 (MQ=255)
                                                                                                  ggggCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATAACGACAAATGCGCCGCCAACCGCGGTAAGAGGAAAGCCCg                                                 >  4:229269/1‑148 (MQ=255)
                                                                                                           cAGAGCAGCAACAAGCCCCCCGTTTCTACAAGCAGAGAGACCATCGCCACGTTCCCGCCGCCAAAACGGTCCGGCATCCCGCCAACCATGACGCGCATCACGACAAATGCGCCGCCACACGCAGGAAGAGTACAGCCCGCCATCGcc                                         >  5:275113/1‑147 (MQ=255)
                                                                                                               gcagcaACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAAt                                                                                >  8:312121/1‑104 (MQ=255)
                                                                                                               gcagcaACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAAt                                                                                <  7:312121/104‑1 (MQ=255)
                                                                                                                 agcaACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACGTTCACGCCGCCAAAACGGTCCGGCATCCACCCAAACATGACGCGCATCACGACAACAGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCtt                                  >  5:11343/1‑148 (MQ=255)
                                                                                                                    aacaaGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCa                                                                                           <  7:146694/88‑1 (MQ=255)
                                                                                                                    aacaaGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCa                                                                                           >  8:146694/1‑88 (MQ=255)
                                                                                                                    aacaaGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGc                               <  8:75339/148‑1 (MQ=255)
                                                                                                                     acaaGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCt                              <  7:222767/148‑1 (MQ=255)
                                                                                                                           ccACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCa                          >  2:156205/1‑146 (MQ=255)
                                                                                                                             aCCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAg                       >  5:229223/1‑147 (MQ=255)
                                                                                                                             aCCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGt                      <  6:172322/148‑1 (MQ=255)
                                                                                                                              ccGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAg                       >  4:255701/1‑146 (MQ=255)
                                                                                                                               cGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAg                       >  3:240104/1‑145 (MQ=255)
                                                                                                                                      aCAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGcgaaa             >  2:200598/1‑148 (MQ=255)
                                                                                                                                       cAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGcgaa              <  6:229223/146‑1 (MQ=255)
                                                                                                                                          gCAGAGAGACAATCTCCACTTTAACGCCGCCAAAAAGGTCCGGCATCCAGACAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGcgaaacgaa         <  2:285652/148‑1 (MQ=255)
                                                                                                                                          gCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGcgaaacgaa         <  7:64482/148‑1 (MQ=255)
                                                                                                                                          gCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGcgaaacgaa         <  1:196832/148‑1 (MQ=255)
                                                                                                                                          gCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGcgaaacgaa         <  2:217536/148‑1 (MQ=255)
                                                                                                                                          gCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGcgaaacgaa         <  1:353088/148‑1 (MQ=255)
                                                                                                                                          gCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGcgaaacga          >  6:89147/1‑147 (MQ=255)
                                                                                                                                           cAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGcgaaacga          >  4:5003/1‑146 (MQ=255)
                                                                                                                                             gagagaCAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGt                                                         >  6:58419/1‑97 (MQ=255)
                                                                                                                                             gagagaCAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGt                                                         <  5:58419/97‑1 (MQ=255)
                                                                                                                                               gagaCAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGCGAAACGAAAGTcc    >  4:60893/1‑148 (MQ=255)
                                                                                                                                                 gaCAATCGCCACTTTCACGCCGCCAAAAGGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACATATGCGCCTCCAAACGCGGTATGAGTTAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTATAGCGAAACGATAGTCCCg  <  6:216512/148‑1 (MQ=255)
                                                                                                                                                 gaCAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCATCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGcgaaacgaa         <  2:90984/141‑1 (MQ=255)
                                                                                                                                                 gaCAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGcgaaacgaa         >  1:90984/1‑141 (MQ=255)
                                                                                                                                                 gaCAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGACAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGCGAAACGAAAGTCCCg  <  6:11343/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCAAACGCGGTAAGAGTAAAGCCCGCCATCGCCCATCCTTTGCTGGCAAAGTAGAGCGAAACGAAAGTCCCG  >  NC_000913/2439124‑2439416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: