Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 324,581 C→T 5.5% intergenic (+128/+115) ykgG → / ← ykgH LutC family protein; putative electron transport chain YkgEFG component/putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913324,5810CT 5.5% 355.7 / 5.4 109intergenic (+128/+115)ykgG/ykgHLutC family protein; putative electron transport chain YkgEFG component/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (58/45);  new base T (4/2);  total (62/47)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01

Only 100 of 111 total aligned reads displayed.

ATTATTGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTA  >  NC_000913/324436‑324727
                                                                                                                                                 |                                                                                                                                                  
attattGAGGATTGTTGAGAGAGATATGAAGTCTGTACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCa                                                                                                                                                   <  5:58671/147‑1 (MQ=255)
 ttattGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTATAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTCGTATTACCAAt                                                                                                                                                 >  3:9318/1‑148 (MQ=255)
   attGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGc                                                                                                                                               >  5:348496/1‑148 (MQ=255)
     tGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGc                                                                                                                                               >  5:316446/1‑146 (MQ=255)
           ttgttgAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGatat                                                                                                                                       <  7:157603/148‑1 (MQ=255)
             gccgcgagacaTATGAAGTCTTGAAATAAACCTGAAGACGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATAttt                                                                                                                                     <  7:288366/143‑1 (MQ=255)
                gagagagaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGCATTGGTAATACCAGGATATTTAc                                                                                                                                   <  3:313787/147‑1 (MQ=39)
                 agagagaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACa                                                                                                                                  <  6:348496/147‑1 (MQ=255)
                 agagagaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAg                                                                                                                                 >  4:12856/1‑148 (MQ=255)
                  gagagaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGCATTGGTAATACCAGGATAttt                                                                                                                                     >  6:299821/1‑143 (MQ=39)
                  gagagaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGCATTGGTAATACCAGGATAttt                                                                                                                                     <  5:299821/143‑1 (MQ=39)
                    gagaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCa                                                                                                                                                   <  6:205802/127‑1 (MQ=255)
                    gagaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCa                                                                                                                                                   >  5:205802/1‑127 (MQ=255)
                      gaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCTAGGATATTTACAGTGaa                                                                                                                             >  5:32643/1‑147 (MQ=255)
                         atGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGaaa                                                                                                                            >  2:280535/1‑145 (MQ=255)
                         atGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGaaa                                                                                                                            >  3:295045/1‑145 (MQ=255)
                          tGAAGTCTGCAAATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCCTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTATTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGa                                                                                                                        <  3:14490/148‑1 (MQ=255)
                           gAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCa                                                                                                                                                   <  8:305558/120‑1 (MQ=255)
                           gAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCa                                                                                                                                                   >  7:305558/1‑120 (MQ=255)
                            aaGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGa                                                                                                                        >  3:32535/1‑146 (MQ=255)
                                cTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGc                                                                                                                   <  7:356619/147‑1 (MQ=255)
                                  ggACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATc                                                                                                                >  8:53183/1‑148 (MQ=255)
                                            cTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGa                                                                                                       >  1:224512/1‑147 (MQ=255)
                                              gaagaaGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGaaaa                                                                                                    >  2:31488/1‑148 (MQ=255)
                                              gaagaaGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGaaaa                                                                                                    >  2:3064/1‑148 (MQ=255)
                                              gaagaaGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGaaaa                                                                                                    >  6:145110/1‑148 (MQ=255)
                                               aagaagGCTTACGAGGTTGAATATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCGAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGaaa                                                                                                     <  4:9318/146‑1 (MQ=255)
                                               aagaagGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGaaaa                                                                                                    <  2:380666/147‑1 (MQ=255)
                                               aagaagGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGaaa                                                                                                     <  1:280535/146‑1 (MQ=255)
                                                     gCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttc                                                                                             <  5:199699/148‑1 (MQ=255)
                                                     gCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttc                                                                                             >  8:1619/1‑148 (MQ=255)
                                                     gCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttc                                                                                             >  8:112121/1‑148 (MQ=255)
                                                        tACGAGGTTGAAAATAGCGTTTAGCACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttccct                                                                                          >  6:160069/1‑148 (MQ=255)
                                                         aCGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGaaa                                                                                                                            <  4:175855/113‑1 (MQ=255)
                                                         aCGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGaaa                                                                                                                            >  3:175855/1‑113 (MQ=255)
                                                         aCGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttccctt                                                                                         >  7:141786/1‑148 (MQ=255)
                                                         aCGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttccctt                                                                                         >  5:219808/1‑148 (MQ=255)
                                                         aCGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttcc                                                                                            <  6:289279/145‑1 (MQ=255)
                                                          cGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACa                                                                                                                                  <  8:152528/106‑1 (MQ=255)
                                                          cGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACa                                                                                                                                  >  7:152528/1‑106 (MQ=255)
                                                          cGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttccctt                                                                                         <  4:295045/147‑1 (MQ=255)
                                                               ttGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCAATTCATGAAAACGGGTTCCCTTCCCGGt                                                                                   >  7:334056/1‑148 (MQ=255)
                                                                tGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTg                                                                                                        >  4:144520/1‑126 (MQ=255)
                                                                tGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTg                                                                                                        <  3:144520/126‑1 (MQ=255)
                                                                tGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCTGt                                                                                   >  5:304904/1‑147 (MQ=255)
                                                                tGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGt                                                                                   >  8:223707/1‑147 (MQ=255)
                                                                  aaaaTAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCa                                                                                 <  7:112121/147‑1 (MQ=255)
                                                                   aaaTAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCAtt                                                                               >  3:289212/1‑148 (MQ=255)
                                                                      tAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTc                                                                            <  5:377769/148‑1 (MQ=255)
                                                                           tttAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACtt                                                                        >  1:136578/1‑147 (MQ=255)
                                                                            ttAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACtt                                                                        >  8:393327/1‑146 (MQ=255)
                                                                            ttAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACtt                                                                        >  4:35199/1‑146 (MQ=255)
                                                                                gACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCtt                                                                  >  5:22420/1‑148 (MQ=255)
                                                                                 aCAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCtt                                                                  >  1:383375/1‑147 (MQ=255)
                                                                                   aaCCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTta                                                                >  8:372498/1‑147 (MQ=255)
                                                                                     ccTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATAc                                                             <  3:35199/148‑1 (MQ=255)
                                                                                      cTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGtt                                                                                              <  8:301048/114‑1 (MQ=255)
                                                                                      cTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGtt                                                                                              >  7:301048/1‑114 (MQ=255)
                                                                                         aCTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGc                                                         <  3:12856/148‑1 (MQ=255)
                                                                                            tAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTc                                                                                  <  7:304863/120‑1 (MQ=255)
                                                                                            tAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCAct                                                      >  3:254486/1‑148 (MQ=255)
                                                                                              aCCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCActct                                                    >  1:394530/1‑148 (MQ=255)
                                                                                              aCCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCActct                                                    >  8:314757/1‑148 (MQ=255)
                                                                                              aCCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCActct                                                    <  7:314757/148‑1 (MQ=255)
                                                                                                 ccGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTa                                                 >  1:347598/1‑148 (MQ=255)
                                                                                                 ccGTTCCGGCATGGTTATGCGGCTCTTCTGTAAATATCCTGGTAGTACCAATGCCAGGAGATTTACAGGGAAAAAGCCAGGCATCCAGTCCGGAAAACGGGGTCCCTTCCCGGTCAGTGGCACTACAGCTTTAGACAGGCAcgctc                                                   >  1:141814/1‑146 (MQ=255)
                                                                                                  cGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTta                                                                <  8:31944/132‑1 (MQ=255)
                                                                                                  cGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTta                                                                >  7:31944/1‑132 (MQ=255)
                                                                                                   gTTGCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATc                                               <  2:254783/148‑1 (MQ=255)
                                                                                                   gTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATc                                               <  1:232138/148‑1 (MQ=255)
                                                                                                     tCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGTTATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTAt                                                >  7:10025/1‑145 (MQ=255)
                                                                                                     tCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGc                                                         >  5:116352/1‑136 (MQ=255)
                                                                                                            tGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAAtgttg                                      <  6:219808/148‑1 (MQ=255)
                                                                                                             ggTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAAtgttgt                                     >  7:150161/1‑148 (MQ=255)
                                                                                                               ttATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGttt                                   <  7:53183/148‑1 (MQ=255)
                                                                                                               ttATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGttt                                   <  7:72571/148‑1 (MQ=255)
                                                                                                               ttATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGttt                                   <  1:3064/148‑1 (MQ=255)
                                                                                                               gtATGCTGCTCTTCTGTAAATATCCTGGTATTTCCAAAGCCTGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACTGGTTCCCTTCCCGGTCTTTGTCACTTCAGCTTTATACAGGCTCTCTCTTTCAGAATGTTGttt                                   <  8:334056/147‑1 (MQ=255)
                                                                                                                  tgctgcTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTttatt                                <  1:375505/148‑1 (MQ=255)
                                                                                                                   gctgctCTTCTGTAAATATCCTGGTATTACCAATGCCAGGTTATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTttatt                                <  8:10025/147‑1 (MQ=255)
                                                                                                                    ctgctCTTCTGTAAATATCCTGGTATTACTAATGCCAGGATATTTACAGTGAACAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTACCCGGTCATTGTAACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATAAc                              >  6:198985/1‑148 (MQ=255)
                                                                                                                    ctgctCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTttatta                               <  7:372498/147‑1 (MQ=255)
                                                                                                                    ctgctCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTAc                              >  8:56046/1‑148 (MQ=255)
                                                                                                                         cttctGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGcc                          >  2:241823/1‑147 (MQ=255)
                                                                                                                           tctGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTttatt                                <  7:144742/139‑1 (MQ=255)
                                                                                                                           tctGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTttatt                                >  8:144742/1‑139 (MQ=255)
                                                                                                                           tctGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGcc                          >  5:301337/1‑145 (MQ=255)
                                                                                                                              gTAAATATCCTGGCATTGGTAATACCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGt                    >  1:246564/1‑148 (MQ=39)
                                                                                                                               tAAATATCCTGGTATTACCAATGCTAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCagag                     <  6:32643/146‑1 (MQ=255)
                                                                                                                               tAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGt                    <  6:22420/147‑1 (MQ=255)
                                                                                                                               tAAATATCCTGGCATTGGTAATACCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGtt                   >  5:308849/1‑148 (MQ=39)
                                                                                                                                   tatCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAAAGTGTTCACTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTttgt               >  6:313710/1‑148 (MQ=255)
                                                                                                                                        tGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTa          >  5:190740/1‑148 (MQ=255)
                                                                                                                                        tGGTATTACCAATGACATGATATTTACAGTGAAAAAGACAGGCACCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATAAGAATGTTATTTATTACTGCCCAGAGTTTTGTTGGTa          >  4:26338/1‑148 (MQ=255)
                                                                                                                                           tATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTc       <  4:347036/148‑1 (MQ=255)
                                                                                                                                              tACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATc    >  3:61921/1‑148 (MQ=255)
                                                                                                                                              tACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATc    >  4:227652/1‑148 (MQ=255)
                                                                                                                                              tACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATc    >  1:213452/1‑148 (MQ=255)
                                                                                                                                                ccAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAACACGGGTTCCCTTCCCGGTCATTGTCCCTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTAATGCCCAGAGTTTTGTTGGTATTCATCTa  <  2:224512/148‑1 (MQ=255)
                                                                                                                                                ccAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCACAGAGTTTTGTTGGTATTCATCTa  >  8:374586/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ATTATTGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTA  >  NC_000913/324436‑324727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: