Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	842,194	C→T	intergenic (‑138/‑138)	mcbA ← / → rlmF	colanic acid mucoidy stimulation protein/23S rRNA m(6)A1618 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	842,194	0	C	T	100.0%	329.6 / NA	93	intergenic (‑138/‑138)	mcbA/rlmF	colanic acid mucoidy stimulation protein/23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (47/46);  total (47/46)

CTTTTTCATAATGACATCCTCATAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGC  >  NC_000913/842048‑842339                                                                                                                                                   |                                                                                                                                                  cTTTTTCATAATGACATCCTCATAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGatat                                                                                                                                                 <  2:230473/149‑1 (MQ=255)        aTAATGACATCCTCATAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCTAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATaa                                                                                                                                           <  8:19696/148‑1 (MQ=255)        aTAATGACATCCTCATAAATACAACGGGTAGTGCCTGATAAGTGTACGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATaa                                                                                                                                           <  4:70755/148‑1 (MQ=255)         tAATGACATCCTCATAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATg                                                                                                                                         <  8:37635/149‑1 (MQ=255)         tAATGACATCCTCATAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATg                                                                                                                                         >  7:81107/1‑149 (MQ=255)          aaTGACATCCTCATAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGc                                                                                                                                        >  8:65689/1‑149 (MQ=255)          aaTGACATCCTCATAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGc                                                                                                                                        >  8:382393/1‑149 (MQ=255)             gACATCCTCATAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGa                                                                                                                                     >  1:262480/1‑149 (MQ=255)                aTCCTCATAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGAttt                                                                                                                                  >  6:258782/1‑149 (MQ=255)                   cTCATAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGa                                                                                                                                     >  5:206651/1‑143 (MQ=255)                   cTCATAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGa                                                                                                                                     <  6:206651/143‑1 (MQ=255)                      aTAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTat                                                                                                                              <  5:98902/147‑1 (MQ=255)                      aTAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTat                                                                                                                              <  6:135461/147‑1 (MQ=255)                       tAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTatgat                                                                                                                           <  8:81107/149‑1 (MQ=255)                         aaTACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGatat                                                                                                                                                 >  2:221455/1‑125 (MQ=255)                         aaTACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGatat                                                                                                                                                 <  1:221455/125‑1 (MQ=255)                          aTACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGaa                                                                                                                        <  5:15620/149‑1 (MQ=255)                            aCAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAAtt                                                                                                                      >  6:21544/1‑149 (MQ=255)                              aaCGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCa                                                                                                                                              >  7:186447/1‑123 (MQ=255)                              aaCGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGc                                                                                                                    >  4:218358/1‑149 (MQ=255)                                  ggTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATa                                                                                                                <  5:258782/149‑1 (MQ=255)                                         ccTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATa                                                                                                         <  2:267450/149‑1 (MQ=255)                                          cTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAg                                                                                                        <  2:260863/149‑1 (MQ=255)                                           tGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGc                                                                                                       >  8:148658/1‑149 (MQ=255)                                              tAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCAt                                                                                                    >  8:118529/1‑149 (MQ=255)                                                 gtgtAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGAttt                                                                                                                                  >  7:54291/1‑116 (MQ=255)                                                 gtgtAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGAttt                                                                                                                                  <  8:54291/116‑1 (MQ=255)                                                 gtgtAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGt                                                                                                  >  7:31013/1‑148 (MQ=255)                                                  tgtAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGtt                                                                                                 >  1:309038/1‑148 (MQ=255)                                                    tAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGAttttt                                                                                                                                >  7:75353/1‑115 (MQ=255)                                                    tAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGAttttt                                                                                                                                <  8:75353/115‑1 (MQ=255)                                                    tAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAg                                                                                              <  8:31013/149‑1 (MQ=255)                                                      ggTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGt                                                                                            >  7:334204/1‑149 (MQ=255)                                                        tCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGt                                                                                                  <  1:94457/141‑1 (MQ=255)                                                        tCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGt                                                                                                  >  2:94457/1‑141 (MQ=255)                                                          gCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGtttt                                                                                         <  8:334204/148‑1 (MQ=255)                                                          gCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTg                                                                                        >  2:380490/1‑149 (MQ=255)                                                          gCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTg                                                                                        <  5:21544/149‑1 (MQ=255)                                                            cTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTatg                                                                                                                             <  6:91461/110‑1 (MQ=255)                                                            cTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTatg                                                                                                                             >  5:91461/1‑110 (MQ=255)                                                            cTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGt                                                                                                  <  7:205917/137‑1 (MQ=255)                                                            cTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGt                                                                                                  >  8:205917/1‑137 (MQ=255)                                                            cTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTggg                                                                                      <  2:309038/149‑1 (MQ=255)                                                            cTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTggg                                                                                      >  6:395248/1‑149 (MQ=255)                                                              gcgcGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTatga                                                                                                                            <  6:290915/109‑1 (MQ=255)                                                              gcgcGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTatga                                                                                                                            >  5:290915/1‑109 (MQ=255)                                                               cgcgATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGaa                                                                                   <  5:396498/149‑1 (MQ=255)                                                                gcgATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTat                                                                                                                              >  5:33122/1‑105 (MQ=255)                                                                gcgATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTat                                                                                                                              <  6:33122/105‑1 (MQ=255)                                                                   aTGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGa                                                                                >  1:269585/1‑148 (MQ=255)                                                                      cAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATaaa                                                                            <  7:118529/149‑1 (MQ=255)                                                                       aaaTGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGCACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAAc                                                                           <  2:262480/149‑1 (MQ=255)                                                                       aaaTGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGcc                                                                                                                   >  3:59144/1‑109 (MQ=255)                                                                       aaaTGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGcc                                                                                                                   <  4:59144/109‑1 (MQ=255)                                                                       aaaTGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAAc                                                                           >  1:185705/1‑149 (MQ=255)                                                                       aaaTGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAAc                                                                           >  1:219335/1‑149 (MQ=255)                                                                       aaaTGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACACTAGCCATGTTAAGGTTTAGGGGAATGATAAAc                                                                           >  4:195592/1‑149 (MQ=255)                                                                          tGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCa                                                                        <  7:148658/149‑1 (MQ=255)                                                                             cGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGc                                                                      <  5:395248/148‑1 (MQ=255)                                                                              gCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGc                                                                      >  3:138310/1‑147 (MQ=255)                                                                               cAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGc                                                                      >  3:346334/1‑146 (MQ=255)                                                                                aaaaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATa                                                                              >  8:104855/1‑137 (MQ=255)                                                                                aaaaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATa                                                                              <  7:104855/137‑1 (MQ=255)                                                                                aaaaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCa                                                                        <  8:267610/143‑1 (MQ=255)                                                                                aaaaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCa                                                                        >  7:267610/1‑143 (MQ=255)                                                                                aaaaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGc                                                                      >  2:65206/1‑145 (MQ=255)                                                                                aaaaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGc                                                                      >  5:121441/1‑145 (MQ=255)                                                                                     aTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATTATATGCATAATGCTGATTTTTATGATGACTTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATAttt                                                             <  3:195592/149‑1 (MQ=255)                                                                                           agagATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATAtt                                                              >  4:273634/1‑142 (MQ=255)                                                                                           agagATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATAtt                                                              <  3:273634/142‑1 (MQ=255)                                                                                             agaTGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTc                                                     <  7:382393/149‑1 (MQ=255)                                                                                             agaTGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTc                                                     >  4:366184/1‑149 (MQ=255)                                                                                             agaTGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACAATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTc                                                     >  2:54202/1‑149 (MQ=255)                                                                                                tgtgCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGaa                                                                                                                        >  7:342792/1‑79 (MQ=255)                                                                                                tgtgCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGaa                                                                                                                        <  8:342792/79‑1 (MQ=255)                                                                                                 gtgCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGt                                                 >  7:150625/1‑149 (MQ=255)                                                                                                   gCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGat                                                                 >  7:201451/1‑131 (MQ=255)                                                                                                   gCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGat                                                                 <  8:201451/131‑1 (MQ=255)                                                                                                     ttATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTg                                                                                        >  7:260384/1‑106 (MQ=255)                                                                                                     ttATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTg                                                                                        <  8:260384/106‑1 (MQ=255)                                                                                                       aTTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGc                                            <  2:219335/148‑1 (MQ=255)                                                                                                        ttttttGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCgg                                          <  1:380490/149‑1 (MQ=255)                                                                                                          ttttGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCggg                                         <  6:121441/148‑1 (MQ=255)                                                                                                             tGAACGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTAt                                      <  4:346334/148‑1 (MQ=255)                                                                                                                aCGATATATTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTa                                  <  4:160966/149‑1 (MQ=255)                                                                                                                      tatTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcgccgc                            <  1:65206/149‑1 (MQ=255)                                                                                                                      tatTTTTACAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcgccgc                            >  1:308047/1‑149 (MQ=255)                                                                                                                             aCAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTa                      >  7:61369/1‑148 (MQ=255)                                                                                                                              cAAAATAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTaaa                    <  2:185705/149‑1 (MQ=255)                                                                                                                               aaaaTAAGACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAg                   <  2:308047/149‑1 (MQ=255)                                                                                                                                      gACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGTGAATGATAAACGCAGCCAGATATTTACGCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATg            <  1:54202/149‑1 (MQ=255)                                                                                                                                      gACAAATCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATg            <  7:343479/149‑1 (MQ=255)                                                                                                                                         aaaTCAATGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTa         >  5:16138/1‑149 (MQ=255)                                                                                                                                          aaTCAATGATATGCATAATGCTCATTTTTATGAGGAATTGCAATAAACCATAGGCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGATAAGCGGGTATCTTACGAAGCTGCTAAAAGGAGAAAGCTAt        >  2:5445/1‑149 (MQ=255)                                                                                                                                               aTGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCa                                                                        >  6:50779/1‑80 (MQ=255)                                                                                                                                                tGATATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGc  >  2:289243/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CTTTTTCATAATGACATCCTCATAAATACAACGGGTAGTGCCTGATAAGTGTAGGTCGCCTGCGCGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGC  >  NC_000913/842048‑842339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑