New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3574971	35 (0.980)	4 (0.120)	4/232	3.3	9.3%	intergenic (‑93/‑100)	asd/yhgN	aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/UPF0056 family inner membrane protein
	?	NC_000913	= 3575007	45 (1.350)					intergenic (‑129/‑64)	asd/yhgN	aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/UPF0056 family inner membrane protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATG  >  NC_000913/3574853‑3575115                                                                                                                       |                                                                                                                                                 cAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCAGGTCTCCTCTTGGCACATCTTTCACCATACATAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCAt                                                                                                                    <  7:91589/149‑1 (MQ=255)           aaCCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCa                                                                                                                  <  4:9884/141‑1 (MQ=255)           aaCCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCa                                                                                                                  >  3:9884/1‑141 (MQ=255)               aaCATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGcc                                                                                                                      <  3:158512/133‑1 (MQ=255)               aaCATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGcc                                                                                                                      >  4:158512/1‑133 (MQ=255)               aaCATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACtt                                                                                                      <  6:80034/149‑1 (MQ=255)                         aTAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCttt                                                                                            >  1:44314/1‑149 (MQ=255)                         aTAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCt                                                                                              >  4:57408/1‑147 (MQ=255)                         aTAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCt                                                                                              <  3:57408/147‑1 (MQ=255)                               gTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAg                                                                                       >  7:11387/1‑148 (MQ=255)                               gTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGc                                                                                      <  8:11387/149‑1 (MQ=255)                                                     tgCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGAAAGTGAAATTAATCAATGATAGCGAGCCCAt                                                                                                                    >  4:143126/1‑97 (MQ=255)                                                     tgCTGTATAAATGTGCCGGTCTCCTCATGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCAt                                                                                                                    <  3:143126/97‑1 (MQ=255)                                                         gTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTAt                                                              >  5:13488/1‑147 (MQ=255)                                                              aaTGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGaaa                                                                             >  8:39032/1‑127 (MQ=255)                                                              aaTGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGaaa                                                                             <  7:39032/127‑1 (MQ=255)                                                              aaTGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGaa                                                       <  6:13488/149‑1 (MQ=255)                                                                     cGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGt                                                <  2:44314/149‑1 (MQ=255)                                                                     cGGTCTCCTATTGGACCATCTTACACCATACAAAAAGCAGCCAAAGACGCAACTGAAATTAATCAAAGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGACCGCGCCAATTATCAGGGAATTTGact                                                >  4:59326/1‑147 (MQ=255)                                                                      ggTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTACGCGGCAATTATCAGGGAATTTGAGt                                                >  4:126103/1‑148 (MQ=255)                                                                            ctctTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCa                                                                                     <  7:76309/105‑1 (MQ=255)                                                                            ctctTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCa                                                                                     >  8:76309/1‑105 (MQ=255)                                                                                  gCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGa                                                  <  7:32558/134‑1 (MQ=255)                                                                                  gCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGa                                                  >  8:32558/1‑134 (MQ=255)                                                                                                    aaaaaGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTa                 >  6:67184/1‑149 (MQ=255)                                                                                                        agcagcCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTgca                          >  6:68519/1‑136 (MQ=255)                                                                                                        agcagcCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTgca                          <  5:68519/136‑1 (MQ=255)                                                                                                         gcagcCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTat                                             <  4:159680/116‑1 (MQ=255)                                                                                                         gcagcCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTat                                             >  3:159680/1‑116 (MQ=255)                                                                                                            gcCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTa                 >  6:56408/1‑141 (MQ=255)                                                                                                            gcCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTa                 <  5:56408/141‑1 (MQ=255)                                                                                                             ccAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGaaa                                     >  4:2389/1‑120 (MQ=255)                                                                                                             ccAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGaaa                                     <  3:2389/120‑1 (MQ=255)                                                                                                                aaGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGAtt     <  5:67184/149‑1 (MQ=255)                                                                                                                   tCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATg  >  5:157974/1‑149 (MQ=255)                                                                                                                       |                                                                                                                                                 CAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAATCAATGATAGCGAAGCCATCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATG  >  NC_000913/3574853‑3575115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.