Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,862,760 C→T R142R (CGC→CGT ygbK → FliA‑regulated DUF1537 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,862,7600CT100.0% 140.2 / NA 41R142R (CGC→CGTygbKFliA‑regulated DUF1537 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/20);  total (21/20)

GCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTAC  >  NC_000913/2862621‑2862899
                                                                                                                                           |                                                                                                                                           
gCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCg                                                                                                                                    >  7:31219/1‑149 (MQ=255)
                cTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCa                                                                                                                          <  1:94040/143‑1 (MQ=255)
                cTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCa                                                                                                                          <  1:96917/143‑1 (MQ=255)
                cTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCa                                                                                                                          >  2:94040/1‑143 (MQ=255)
                cTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCa                                                                                                                          >  2:96917/1‑143 (MQ=255)
                     tGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCa                                                                                                                          >  3:44852/1‑138 (MQ=255)
                     tGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCa                                                                                                                          <  4:44852/138‑1 (MQ=255)
                        aTGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATc                                                                                                                             >  3:1450/1‑132 (MQ=255)
                        aTGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATc                                                                                                                             <  4:1450/132‑1 (MQ=255)
                                              ggTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCAt                                                                                                                         >  6:120553/1‑114 (MQ=255)
                                                tcttctCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCa                                                                                                                          <  1:52865/111‑1 (MQ=255)
                                                tcttctCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCa                                                                                                                          >  2:52865/1‑111 (MQ=255)
                                                  ttctCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATc                                                                                  >  4:149121/1‑149 (MQ=255)
                                                              cTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGaa                                                                                          >  6:108358/1‑129 (MQ=255)
                                                              cTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGaa                                                                                          <  5:108358/129‑1 (MQ=255)
                                                                gCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTa                                                                                                                                 <  7:14080/88‑1 (MQ=255)
                                                                gCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTa                                                                                                                                 >  8:14080/1‑88 (MQ=255)
                                                                       aaCGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTTCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAg                                                                                                                <  6:12207/98‑1 (MQ=255)
                                                                       aaCGGACGTACGGTTTATAAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGAGTCATCACCCGGTAAATCCCATGACCGACAg                                                                                                                >  5:12207/1‑98 (MQ=255)
                                                                            aCGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCcaca                                                                              <  1:153153/127‑1 (MQ=255)
                                                                            aCGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCcaca                                                                              >  2:153153/1‑127 (MQ=255)
                                                                                aCGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCCTGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGcaca                                                    >  4:16538/1‑149 (MQ=255)
                                                                                   gTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCt                                                                                                              >  6:123188/1‑88 (MQ=255)
                                                                                   gTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCt                                                                                                              <  5:123188/88‑1 (MQ=255)
                                                                                        tCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACa                                                                                                                 <  5:38460/80‑1 (MQ=255)
                                                                                        tCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACa                                                                                                                 >  6:38460/1‑80 (MQ=255)
                                                                                          aGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGcgtc                                                               >  3:11277/1‑128 (MQ=255)
                                                                                          aGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCACATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGcgtc                                                               <  4:11277/128‑1 (MQ=255)
                                                                                              gTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTc                                                          <  4:56482/129‑1 (MQ=255)
                                                                                              gTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTc                                                          >  3:56482/1‑129 (MQ=255)
                                                                                              gTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAg                                      <  3:16538/149‑1 (MQ=255)
                                                                                                   tGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgtgt                                  <  3:149121/148‑1 (MQ=255)
                                                                                                          aaTGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCg                          >  2:24348/1‑149 (MQ=255)
                                                                                                           aTGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGGTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCg                          <  8:31219/148‑1 (MQ=255)
                                                                                                                tCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCgg                                                        >  5:153357/1‑113 (MQ=255)
                                                                                                                tCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCgg                                                        <  6:153357/113‑1 (MQ=255)
                                                                                                                              aaTCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTc                    <  2:8646/135‑1 (MQ=255)
                                                                                                                              aaTCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTc                    >  1:8646/1‑135 (MQ=255)
                                                                                                                                  cGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTAc  <  1:24348/149‑1 (MQ=255)
                                                                                                                                      aTGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTc                       <  1:144108/124‑1 (MQ=255)
                                                                                                                                      aTGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTc                       >  2:144108/1‑124 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTAC  >  NC_000913/2862621‑2862899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: