| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,767,182 | Δ1 bp | coding (1504/3057 nt) | ydiJ ← | putative FAD‑linked oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,767,180 | 0 | T | . | 78.6% | 117.9 / 61.1 | 58 | coding (1506/3057 nt) | ydiJ | putative FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (6/6); new base . (24/20); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
CTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACC > NC_000913/1767032‑1767326 | cTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCt < 5:384486/149‑1 (MQ=255) tcatcaTCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCttt < 3:126150/148‑1 (MQ=255) aTCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGccc > 2:181273/1‑148 (MQ=255) agaCCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCccaa > 1:402285/1‑149 (MQ=255) ccTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACaaa < 2:382099/149‑1 (MQ=255) ttCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCATCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAacc > 3:79815/1‑149 (MQ=255) tGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGGATTCAGCGCG‑AAACCCTTGCCGTGCTCGCCCCACAACAAaccacc > 7:341387/1‑149 (MQ=255) gCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAaccac < 4:190314/146‑1 (MQ=255) gggCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTAt > 3:235678/1‑149 (MQ=255) gggCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTAt > 4:343485/1‑149 (MQ=255) aaaTCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCg > 8:123165/1‑149 (MQ=255) aTCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCGCAACAAACCACCGTATTTCg > 2:283848/1‑147 (MQ=255) aTCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCa < 3:219858/149‑1 (MQ=255) cTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAaccacc > 2:234675/1‑137 (MQ=255) cTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAaccacc < 1:234675/137‑1 (MQ=255) ttCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTc > 8:61155/1‑149 (MQ=255) cccTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCa < 7:268829/145‑1 (MQ=255) cTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAgc < 8:71900/149‑1 (MQ=255) tGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAgcgc > 2:75062/1‑149 (MQ=255) ggTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGcca > 4:42692/1‑149 (MQ=255) gTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGccc < 4:268959/109‑1 (MQ=255) gTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGccc > 3:268959/1‑109 (MQ=255) ggTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAACCGCCTGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGtcat < 7:405994/149‑1 (MQ=255) ggTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCCCGtca > 4:144829/1‑149 (MQ=255) gTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGtcat < 2:308645/149‑1 (MQ=255) tGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCaga < 3:343485/148‑1 (MQ=255) tCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTg > 3:141367/1‑149 (MQ=255) aaaTGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCCCCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCtt > 1:56334/1‑149 (MQ=255) aaaTGACGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAA‑CATTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACAACGTCATCAGAGATTTGCtt > 5:181831/1‑149 (MQ=255) aaTGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCATCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCCCAACCAACCCCCGTATTTCGCAGTCAGCGCCCCCACGTCATCAGAGATTTGCtg > 8:281761/1‑147 (MQ=255) aTGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCCGCGCGAAAACC‑TTGCCGTGCTCGCCACCCAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGACAGCACAGAATTGCtt > 4:106335/1‑147 (MQ=255) ccGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTtcat < 1:181273/148‑1 (MQ=255) ccGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTtcatc > 2:183930/1‑148 (MQ=255) ccGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTtcatc < 4:235678/148‑1 (MQ=255) tttCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAAt > 7:203712/1‑149 (MQ=255) cTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCtt < 7:61155/149‑1 (MQ=255) tGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCttgt < 3:44374/148‑1 (MQ=255) cAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCCAAATCTCTTGTTGAGGATCGcaaa > 8:153775/1‑147 (MQ=255) cAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAg > 8:399273/1‑140 (MQ=255) cAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAg < 7:399273/140‑1 (MQ=255) cAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGcaca > 3:306748/1‑149 (MQ=255) gTTCCTCACCGAAAAACGCCGGGCTGTATTAAGCGCGAAAACC‑TTGCAGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCAACACCTCATCAGAGATTTGCTTCATCAAAAACTCTTCTTGAGGATCTCACATATcaa > 7:330927/1‑147 (MQ=255) tCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGa < 2:383395/129‑1 (MQ=255) tCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGa > 1:383395/1‑129 (MQ=255) tCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGAt > 5:24700/1‑132 (MQ=255) tCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGAt < 6:24700/132‑1 (MQ=255) aCCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTg < 4:79815/148‑1 (MQ=255) aCCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTg > 3:100461/1‑148 (MQ=255) cGCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTg < 3:42692/149‑1 (MQ=255) gCCGGGCTGTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGc < 1:183930/149‑1 (MQ=255) gTATTCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGACAcc > 6:357495/1‑149 (MQ=255) ttCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCttg > 7:132752/1‑101 (MQ=255) ttCAGCGCGAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCttg < 8:132752/101‑1 (MQ=255) gcgAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGAc < 1:283848/149‑1 (MQ=255) cgAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGAGCGCACATATCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGAc < 2:402285/149‑1 (MQ=255) gAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACCTATCCAGCGCTGGACGGCCGTGCAAGACACCTGCGTCGACGt > 6:352067/1‑149 (MQ=255) gAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGt < 1:75062/149‑1 (MQ=255) gAAAACC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGt < 2:56334/149‑1 (MQ=255) aaaCC‑TTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGa < 8:203712/149‑1 (MQ=255) cTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGAcc > 6:177491/1‑148 (MQ=255) | CTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACC > NC_000913/1767032‑1767326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |