New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4559490 =	45 (1.410)	6 (0.200)	5/248	2.9	12.9%	coding (37/1173 nt)	iadA	isoaspartyl dipeptidase
	?	NC_000913	4559512 =	38 (1.240)					coding (15/1173 nt)	iadA	isoaspartyl dipeptidase
Rejected: Frequency below/above cutoff threshold.

TGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTCGGCTGTCCCCAGG  >  NC_000913/4559376‑4559641                                                                                                                                         |                                                                                                                                  tGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATaatcaatc                                                                                                                       <  1:38248/149‑1 (MQ=255)       gccaaatattacccgcatatcattacggtctgcgtgattaacgcattgctgtcgatctgggttatgcagcttattgtttaacaaggagttaccatgattgattataccGCAGCAGGGTAAAACCGGCTGCGGt                                                                                                                                 >  3:71193‑M1/109‑133 (MQ=255)       gccaaatattacccgcatatcattacggtctgcgtgattaacgcattgctgtcgatctgggttatgcagcttattgtttaacaaggagttaccatgattgattataccGCAGCAGGGTAAAACCGGCTGCGGt                                                                                                                                 <  4:71193‑M1/25‑1 (MQ=255)           tACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGAGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTc                                                                                                             >  7:42976/1‑149 (MQ=255)            aCAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGt                                                                                                                  >  8:72240/1‑143 (MQ=255)            aCAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGt                                                                                                                  <  7:72240/143‑1 (MQ=255)                    aGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAatcaatca                                                                                                                      <  6:87442/131‑1 (MQ=255)                    aGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAatcaatca                                                                                                                      >  5:87442/1‑131 (MQ=255)                          gATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTg                                                                                                         >  6:93891/1‑138 (MQ=255)                          gATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTg                                                                                                         <  5:93891/138‑1 (MQ=255)                           atatTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAg                                                                                             >  3:107504/1‑149 (MQ=255)                                  tGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATAGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCAt                                                                                       >  1:151970/1‑148 (MQ=255)                                    gCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAAc                                                                                    <  4:107504/149‑1 (MQ=255)                                          gattaacgcattgctgtcgatctgggttatgcagcttattgtttaacaaggagttaccatgattgattataccGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGtt                                                                                                       <  4:68028‑M1/51‑1 (MQ=255)                                          gattaacgcattgctgtcgatctgggttatgcagcttattgtttaacaaggagttaccatgattgattataccGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGtt                                                                                                       >  3:68028‑M1/74‑124 (MQ=255)                                            gATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATaat                                                                                                                            <  2:134216/101‑1 (MQ=255)                                            gATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATaat                                                                                                                            >  1:134216/1‑101 (MQ=255)                                            gATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATc                                                                             >  7:102869/1‑148 (MQ=255)                                            gATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATc                                                                             <  8:102869/148‑1 (MQ=255)                                             aTAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGa                                                                           >  3:77687/1‑149 (MQ=255)                                                  tttGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTcc                                                                                                            <  3:103499/111‑1 (MQ=255)                                                  tttGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTcc                                                                                                            >  4:103499/1‑111 (MQ=255)                                                        gTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAAcc                                                                                   >  1:16833/1‑130 (MQ=255)                                                        gTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAAcc                                                                                   <  2:16833/130‑1 (MQ=255)                                                                 gaGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGc                                                       <  4:77687/149‑1 (MQ=255)                                                                        tCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGc                                                                                         <  3:82986/108‑1 (MQ=255)                                                                        tCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGc                                                                                         >  4:82986/1‑108 (MQ=255)                                                                        tCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCAGACCg                                                 >  2:36632/1‑148 (MQ=255)                                                                          gCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCa                                                      >  6:149367/1‑141 (MQ=255)                                                                          gCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCa                                                      <  5:149367/141‑1 (MQ=255)                                                                           cAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCAGACCGTAAt                                             <  8:42976/149‑1 (MQ=255)                                                                                  cccGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGc                                      <  1:36632/149‑1 (MQ=255)                                                                                  cccGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGc                                      >  2:37498/1‑149 (MQ=255)                                                                                   ccGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAg                                                                        >  1:113999/1‑114 (MQ=255)                                                                                   ccGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAg                                                                        <  2:113999/114‑1 (MQ=255)                                                                                      aacaaggagttaccatgattgattataccgcagccggttttaccctgctgcGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATc                                                           <  4:68028‑M2/73‑1 (MQ=255)                                                                                      aacaaggagttaccatgattgattataccgcagccggttttaccctgctgcGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATc                                                           >  3:68028‑M2/52‑124 (MQ=255)                                                                                         ttCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAAc                                                                                                   >  2:105879/1‑81 (MQ=255)                                                                                         ttCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAAc                                                                                                   <  1:105879/81‑1 (MQ=255)                                                                                                      aaaTGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCTTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCAc                  <  2:151970/149‑1 (MQ=255)                                                                                                      aaaTGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCAc                  <  1:37498/149‑1 (MQ=255)                                                                                                                ccTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCGTGTTAAACAATAAGCTGCATAACCCAGCTCGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTCGGCTGTc        >  3:97873/1‑149 (MQ=255)                                                                                                                accgcagccggttttaccctgctgcGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGc                        <  4:71193‑M2/108‑1 (MQ=255)                                                                                                                accgcagccggttttaccctgctgcGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGc                        >  3:71193‑M2/26‑133 (MQ=255)                                                                                                                  tgcagccggttttaccctgctgcGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACa                                                                         >  2:57516‑M2/24‑82 (MQ=255)                                                                                                                  tgcagccggttttaccctgctgcGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACa                                                                         <  1:57516‑M2/59‑1 (MQ=255)                                                                                                                           gTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTCGGCTGTCCCCAgg  <  4:18215/144‑1 (MQ=255)                                                                                                                           gTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTCGGCTGTCCCCAgg  >  3:18215/1‑144 (MQ=255)                                                                                                                                         |                                                                                                                                  TGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTCCCTGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTCGGCTGTCCCCAGG  >  NC_000913/4559376‑4559641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.