Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,032,3360GC21.2% 74.8 / 17.5 33intergenic (‑19/+48)dcm/yedJDNA cytosine methyltransferase/putative HD superfamily phosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (12/14);  new base C (4/3);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTC  >  NC_000913/2032195‑2032473
                                                                                                                                             |                                                                                                                                         
aCCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATgg                                                                                                                                     <  5:58521/148‑1 (MQ=255)
aCCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGc                                                                                                                                    >  3:8706/1‑149 (MQ=255)
      acCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGATGGCCggt                                                                                                                                <  5:107921/147‑3 (MQ=255)
      acCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGATGGCCggt                                                                                                                                >  6:107921/1‑145 (MQ=255)
       ccATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAgg                                                                                                                             >  2:103364/1‑149 (MQ=255)
            aaGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTaa                                                                                                                         <  4:8706/148‑1 (MQ=255)
                 gcgcCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAAt                                                                                                                   >  2:126111/1‑149 (MQ=255)
                     caccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACa                                                                                                               <  1:103364/149‑1 (MQ=255)
                     caccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACa                                                                                                               <  3:46380/149‑1 (MQ=255)
                     caccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCCGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTa                                                                                                                 >  7:39240/1‑147 (MQ=255)
                                  aCATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGc                                                                                                   <  1:126111/148‑1 (MQ=255)
                                                 agcagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcagccagc                                                                                   >  5:116629/1‑149 (MQ=255)
                                                            cAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCCGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAAc                                                                         <  8:39240/148‑1 (MQ=255)
                                                             aGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCAtt                                                                                                         <  2:63662/115‑1 (MQ=255)
                                                             aGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCAtt                                                                                                         >  1:63662/1‑115 (MQ=255)
                                                                                        cTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTc                                            <  6:116629/149‑1 (MQ=255)
                                                                                         tGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATc                                                                <  7:38202/128‑1 (MQ=255)
                                                                                         tGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATc                                                                >  8:38202/1‑128 (MQ=255)
                                                                                                           atatatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGtt                           <  8:98869/147‑1 (MQ=255)
                                                                                                             atatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGac                                                  >  2:60292/1‑122 (MQ=255)
                                                                                                             atatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGac                                                  <  1:60292/122‑1 (MQ=255)
                                                                                                              tatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCa                      >  8:149542/1‑149 (MQ=255)
                                                                                                               atTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCt                                                            <  4:126489/110‑1 (MQ=255)
                                                                                                               atTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCt                                                            >  3:126489/1‑110 (MQ=255)
                                                                                                               atTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTtgtg                                                        <  5:188070/114‑1 (MQ=255)
                                                                                                               atTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTtgtg                                                        >  6:188070/1‑114 (MQ=255)
                                                                                                                             aTTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATc                                                                <  4:120840/92‑1 (MQ=255)
                                                                                                                             aTTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATc                                                                >  3:120840/1‑92 (MQ=255)
                                                                                                                                ctgtcGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCAt        >  2:163837/5‑145 (MQ=255)
                                                                                                                                ctgtcGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCAt        <  1:163837/141‑1 (MQ=255)
                                                                                                                                 tgtcGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTcc                                     >  7:22777/4‑115 (MQ=255)
                                                                                                                                 tgtcGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTcc                                     <  8:22777/112‑1 (MQ=255)
                                                                                                                                   tcGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTc  >  1:65500/2‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                         
ACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTC  >  NC_000913/2032195‑2032473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑