Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	756,396	Δ1 bp	coding (490/1767 nt)	sdhA →	succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,396	0	T	.	97.1%	298.7 / 1.6	70	coding (490/1767 nt)	sdhA	succinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base . (35/33);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCC  >  NC_000913/756253‑756539                                                                                                                                                |                                                                                                                                                aaCGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATg                                                                                                                                           >  7:152694/1‑149 (MQ=255) aaCGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATg                                                                                                                                           >  2:365862/1‑149 (MQ=255) aaCGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATg                                                                                                                                           >  5:190768/1‑149 (MQ=255)     tCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCt                                                                                                                                       >  1:319070/1‑149 (MQ=255)         tttGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGAt                                                                                                                                   >  2:19426/1‑149 (MQ=255)             gCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTgg                                                                                                                               <  5:142791/149‑1 (MQ=255)              cGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGt                                                                                                                              <  8:278894/149‑1 (MQ=255)              cGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGt                                                                                                                              <  1:365862/149‑1 (MQ=255)              cGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACGGCGGCAGCAGCTGACCGTACCGGGCACGCACTGTTGCACACGCTTTATCAGCAGACCCTGAACAACCACACCACCATTTTCTCCGAG‑GGGATGCGCTGGAGCTGGt                                                                                                                              <  8:322637/149‑1 (MQ=255)                     tCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCCCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACCCCACCATTTTCTCCGAG‑GGGATGCGCTGGATCTGGTGaacaac                                                                                                                       >  8:6107/1‑149 (MQ=255)                     tCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAAc                                                                                                                       >  6:402485/1‑149 (MQ=255)                        aaaaaCTGCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCt                                                                                                                                 <  1:239005/136‑1 (MQ=255)                        aaaaaCTCCGGCGGCGAGCAGGCGGCTCGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAGCCACCGCACCATTTTCTCCGAG‑GGTATGCGCTGGATCt                                                                                                                                 >  2:239005/1‑136 (MQ=255)                         aaacCTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAg                                                                                                                    >  5:21561/1‑148 (MQ=255)                          aaaCTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGa                                                                                                                  >  4:223254/1‑149 (MQ=255)                              ttCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAgg                                                                                                                   <  8:59954/144‑1 (MQ=255)                              ttCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAgg                                                                                                                   >  7:59954/1‑144 (MQ=255)                                         gCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTggg                                                                                                   <  3:223254/149‑1 (MQ=255)                                         gCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTggg                                                                                                   >  3:173449/1‑149 (MQ=255)                                           aGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGtt                                                                                                 <  5:402485/149‑1 (MQ=255)                                           aGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGtt                                                                                                 >  6:206037/1‑149 (MQ=255)                                            ggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTg                                                                                                <  2:377593/149‑1 (MQ=255)                                                gcacgcacTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCCCCATTTTCTCCGAG‑GGTCTGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTAcc                                                                                            <  7:6107/149‑1 (MQ=255)                                                    gcacTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCAc                                                                                        >  8:303376/1‑149 (MQ=255)                                                     cacTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACt                                                                                       <  6:21561/149‑1 (MQ=255)                                                      acTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGCTGGGTGGTACCGCACtg                                                                                      >  2:323866/1‑149 (MQ=255)                                                       cTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACtgt                                                                                     <  2:319160/149‑1 (MQ=255)                                                           ggCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCAt                                                                                 <  2:319070/149‑1 (MQ=255)                                                            gcagcaGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATc                                                                                <  8:419042/149‑1 (MQ=255)                                                            gcagcaGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATc                                                                                <  8:152694/149‑1 (MQ=255)                                                                     gACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGt                                                                       <  7:314516/149‑1 (MQ=255)                                                                      aCCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGt                                                                       <  4:173449/148‑1 (MQ=255)                                                                        cGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCCCACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATCGCGCGGTGCTGGGTTGTACCGCACTGTGCATCGAACCCGGTg                                                                      >  8:340906/1‑147 (MQ=255)                                                                           aCCGGTCTCGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTAt                                                                                                                                            <  3:160635/74‑1 (MQ=255)                                                                           aCCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTAt                                                                                                                                            >  4:160635/1‑74 (MQ=255)                                                                                   cGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTAttt                                                         <  1:19426/149‑1 (MQ=255)                                                                                   cGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTAttt                                                         <  3:127662/149‑1 (MQ=255)                                                                                      aCTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCa                                                       >  2:64062/1‑148 (MQ=255)                                                                                             cacaCGCTTTATCAGCAGAACCTGAAAAACCACACCACTATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCcgc                                               >  8:60329/1‑149 (MQ=255)                                                                                              acacGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGt                                                               <  6:298725/132‑1 (MQ=255)                                                                                              acacGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACAAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGt                                                               >  5:298725/1‑132 (MQ=255)                                                                                                acGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCgcgc                                             >  5:96083/1‑148 (MQ=255)                                                                                                acGCTTCATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCgcgc                                             >  1:243744/1‑148 (MQ=255)                                                                                                    tttATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCg                                        >  5:132331/1‑149 (MQ=255)                                                                                                       aTCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGc                                     >  8:211594/1‑149 (MQ=255)                                                                                                           gcagAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCTAAACCGGTGAAGTGGTTTATTGCAAAGCCCGCGCTACCGTGCTGGc                                 >  7:223097/1‑149 (MQ=255)                                                                                                           gcagAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGc                                 >  6:315523/1‑149 (MQ=255)                                                                                                              gAACCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGAc                              >  3:387292/1‑149 (MQ=255)                                                                                                                aCCTGAAAAACCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAATCCGGTGAAGTGGTTTATTTCTAAGCCCGCGCTACCGTGCTGGCGACTg                            >  2:376625/1‑149 (MQ=255)                                                                                                                     aaaaaCCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGa                       <  5:206037/149‑1 (MQ=255)                                                                                                                        aaCCACACCACCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCa                    <  2:45141/149‑1 (MQ=255)                                                                                                                         accacaccacCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGGGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAg                   <  1:200329/149‑1 (MQ=255)                                                                                                                          ccacaccacCATTTTGTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAgg                  <  2:243744/149‑1 (MQ=255)                                                                                                                          ccacaccacCATTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAgg                  >  6:300285/1‑149 (MQ=255)                                                                                                                           caccccacCATTTTCTCCGACTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGGACCGCACTGTGCATCGAAACCGGGGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAgg                  <  4:319210/149‑1 (MQ=255)                                                                                                                               ccaccaTTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTa             <  4:397112/149‑1 (MQ=255)                                                                                                                                caccaTTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTAt            <  2:379089/149‑1 (MQ=255)                                                                                                                                caccaTTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTGGTACCGCACTGTGCATCGATACCGGTGAAGTGGTTTATTTCACCGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTAt            >  4:294804/1‑149 (MQ=255)                                                                                                                                 accaTTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTAtt           >  5:13502/1‑149 (MQ=255)                                                                                                                                 accaTTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTAtt           >  5:104959/1‑149 (MQ=255)                                                                                                                                 accaTTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTAtt           >  8:181610/1‑149 (MQ=255)                                                                                                                                    aTTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTAt        >  3:21324/1‑149 (MQ=255)                                                                                                                                    aTTTTCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGGGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTAt        <  7:340906/149‑1 (MQ=255)                                                                                                                                    aTTTTCTACGA‑TGGTATGCGCTTGATCTGGTGAAAAACCAGGATTGCGCGGTTGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTAAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTAt        <  1:231591/149‑1 (MQ=255)                                                                                                                                       ttCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCTAAACCGGTGAAGTGGTTTATTTCAATGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCa      <  1:321591/148‑1 (MQ=255)                                                                                                                                       ttCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCa      <  5:300285/148‑1 (MQ=255)                                                                                                                                       ttCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCa      <  5:315523/148‑1 (MQ=255)                                                                                                                                       ttCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCa      <  6:132331/148‑1 (MQ=255)                                                                                                                                       ttCTCCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCa      <  6:13502/148‑1 (MQ=255)                                                                                                                                          tcCGAG‑GGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTAGTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTcc  >  2:283594/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                AACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCC  >  NC_000913/756253‑756539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑