Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,215,9890GC22.2% 59.2 / 13.6 27P168R (CCG→CGG) yqjHputative siderophore interacting protein
Reads supporting (aligned to +/- strand):  ref base G (10/11);  new base C (2/4);  total (12/15)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTG  >  NC_000913/3215841‑3216137
                                                                                                                                                    |                                                                                                                                                    
gTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCg                                                                                                                                                      <  3:128175/149‑1 (MQ=255)
    tcatcGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCCGtt                                                                                                                                                   >  5:7552/1‑148 (MQ=255)
    tcatcGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCCGtt                                                                                                                                                   >  5:5160/1‑148 (MQ=255)
                aTTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTATCCAGCGCACTAACTTGCCGTTAAACCGCAAGtta                                                                                                                                      <  6:7552/149‑2 (MQ=255)
                aTTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCCGTTAAACCGCAAGtta                                                                                                                                      <  8:41759/149‑2 (MQ=255)
                aTTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCCGTTAAACCGCAAGtta                                                                                                                                      <  6:5160/149‑2 (MQ=255)
                 tttGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTg                                                                                                                                     >  5:49284/1‑149 (MQ=255)
                 tttGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCCGTTAAACCGCAAGttag                                                                                                                                     <  3:70131/149‑3 (MQ=255)
                      aTCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCaa                                                                                                                                <  1:179914/149‑1 (MQ=255)
                                gACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAgg                                                                                                                      <  4:31214/149‑1 (MQ=255)
                                      cATCTACCGCCTGCTCATCATGGGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAggcggcgg                                                                                                                <  8:116501/149‑1 (MQ=255)
                                               ccTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATg                                                                                                         >  8:136791/1‑147 (MQ=255)
                                                cTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCTAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAg                                                                                                      <  7:136791/149‑1 (MQ=255)
                                                   cTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAg                                                                                                      >  2:34445/1‑146 (MQ=255)
                                                   cTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAg                                                                                                      <  1:34445/146‑1 (MQ=255)
                                                                              aaaaCCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGc                                                                         <  6:49284/148‑1 (MQ=255)
                                                                                     tCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACa                                                                           <  5:72002/139‑1 (MQ=255)
                                                                                     tCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACa                                                                           >  6:72002/1‑139 (MQ=255)
                                                                                                              aGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGaa                                        >  2:5616/1‑149 (MQ=255)
                                                                                                                        ccGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGAc                              >  5:58418/1‑149 (MQ=255)
                                                                                                                         cGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATa                                                                             <  5:59364/101‑1 (MQ=255)
                                                                                                                         cGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATa                                                                             >  6:59364/1‑101 (MQ=255)
                                                                                                                         cGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGAcc                             >  1:85209/1‑149 (MQ=255)
                                                                                                                          gCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCt                            <  1:5616/149‑1 (MQ=255)
                                                                                                                              gCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACAt                                                                              >  4:117482/1‑95 (MQ=255)
                                                                                                                              gCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACAt                                                                              <  3:117482/95‑1 (MQ=255)
                                                                                                                                   ccAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTc                                                        >  4:88115/1‑112 (MQ=255)
                                                                                                                                                  gCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCgg     <  6:58418/148‑1 (MQ=255)
                                                                                                                                                    ggTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTg  >  1:25792/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTG  >  NC_000913/3215841‑3216137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑