Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,947,465	Δ1 bp	10.0%	intergenic (+2/‑32)	metZ → / → metW	tRNA‑Met/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,947,465	0	T	.	10.0%	153.5 / 10.1	50	intergenic (+2/‑32)	metZ/metW	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (14/31);  new base . (2/3);  total (16/34)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGT  >  NC_000913/2947318‑2947547                                                                                                                                                    |                                                                                   cAAATCAGTGATCGGGTTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCCTCCACGGCCTCTGGGTGGCTCAGCCTGGAATCTCTTCTGGCTCATAAACCGAAGGTCGTCGGTTCAAATCAGGACACCGCAACCAAt                                                                                    >  8:459275/1‑148 (MQ=21)  aaaTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAtt                                                                                   <  1:189652/148‑1 (MQ=255)       cGTGATCGGTGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaaa                                                                               <  8:433000/147‑1 (MQ=255)       cGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaat                                                                               <  1:169018/147‑2 (MQ=255)        gTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAtt                                                                             <  7:477057/148‑1 (MQ=255)           ctCGGGGTAACAACACGGGTGCATGAACCCGCGAGCGGAGTATAGCGCGCATCCTCGGTCGCGGGGGGGAGCAGCCTGGGAGCTCGTCGGGCTCATACCCCGAAGGTCTTCGGTTCATATCCGGCCCCCGCAACCAATTAAAATTtg                                                                           <  4:319069/146‑1 (MQ=18)           agcgtgttAAAAATAAGGTTGCATGAAATCTCTATCGTAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtga                                                                          <  2:217280/140‑1 (MQ=17)           aTCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtg                                                                           <  6:373148/147‑1 (MQ=255)           aTCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtg                                                                           <  8:129350/147‑1 (MQ=255)              ggggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCGCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgatga                                                                       <  7:482188/148‑1 (MQ=255)                 gTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGt                                                                    <  7:352564/148‑1 (MQ=255)                 gTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGt                                                                    <  3:314630/148‑1 (MQ=255)                   aaaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTa                                                                   >  2:334603/1‑147 (MQ=255)                    aaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTaa                                                                  <  1:95449/147‑1 (MQ=255)                      aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgatga                                                                       >  2:234316/1‑140 (MQ=255)                      aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgatga                                                                       <  1:234316/140‑1 (MQ=255)                      aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGc                                                               <  1:334603/148‑1 (MQ=255)                         aaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGt                                                            <  8:380165/148‑1 (MQ=255)                           ggTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTAc                                                          <  4:247443/148‑1 (MQ=255)                             ttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACgg                                                        <  4:276016/148‑1 (MQ=255)                                 aTGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAttt                                                                            <  7:58078/124‑1 (MQ=255)                                 aTGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAttt                                                                            >  8:58078/1‑124 (MQ=255)                                  tGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtg                                                                           <  3:175918/124‑1 (MQ=255)                                  tGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtg                                                                           >  4:175918/1‑124 (MQ=255)                                  tGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACg                                                         >  7:454018/1‑142 (MQ=255)                                  tGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACg                                                         <  8:454018/142‑1 (MQ=255)                                  tGAAAAAGAGAGCGTAGTATATTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAACAA‑TCAAATTTGATGAAGTaaaagc                                                              <  5:282557/136‑4 (MQ=14)                                   gAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAACAA‑TAAAATTTTATGAATTaaaagca                                                             >  6:282557/1‑132 (MQ=17)                                    aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGAcgcg                                                 <  2:1690/148‑1 (MQ=255)                                      aaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCggg                                               <  7:279170/148‑1 (MQ=255)                                       aCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTaaaagc                                                              <  1:207048/131‑4 (MQ=255)                                       aCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTaaaagc                                                              >  2:207048/1‑128 (MQ=255)                                        ctctaGCGTAGTATAGTGAGCATCCTCTGACGAGGTGTGTAGCAGCCTGGTATCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCATTACGGTGACGCGGGGt                                             <  5:296279/144‑1 (MQ=17)                                          cgAGTGGAGTAAAGTGCGCATCCAAGGACGCGGGGTTTAGCAGCATGGTAGCTAGTGTGTCTAATAACCCGAAGGTCGGCGGTTCAAATCCGACCCCCTCAACCAATTATAATTTGATGAAGTAAAGCAGTACTGTGACGCGGGGTgg                                           <  8:248020/148‑1 (MQ=255)                                                              tCCACGGACGCGGGGTGTAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGt                                                       <  7:327204/116‑1 (MQ=255)                                                                  cGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGTCGCgg                                                <  8:294913/119‑1 (MQ=255)                                                                  cGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCgg                                                >  7:294913/1‑119 (MQ=255)                                                                                             cTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTgg                                 <  4:122061/107‑1 (MQ=255)                                                                                             cTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTgg                                 >  3:122061/1‑107 (MQ=255)                                                                                                       tCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCggg                                               <  6:235409/83‑1 (MQ=255)                                                                                                       tCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCggg                                               >  5:235409/1‑83 (MQ=255)                                                                                                        cATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGc                                                               <  1:173945/66‑1 (MQ=255)                                                                                                        cATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGc                                                               >  2:173945/1‑66 (MQ=255)                                                                                                        cATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTAATAAATTAAAGCAGTACGGTGACGCGGGGTGGagc                                        >  2:353724/1‑89 (MQ=14)                                                                                                         aTAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAg                                                             >  5:131636/1‑67 (MQ=255)                                                                                                         aTAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAg                                                             <  6:131636/67‑1 (MQ=255)                                                                                                                         cgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAg                                                                <  6:68573/48‑1 (MQ=255)                                                                                                                         cgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAg                                                                >  5:68573/1‑48 (MQ=255)                                                                                                                                                 cAATTAAAATTTTATTAATTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCt                                   <  1:419033/53‑1 (MQ=17)                                                                                                                                                  aaTTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTg                                  >  2:419033/1‑53 (MQ=255)                                                                                                                                                  aaTTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt  >  8:188883/1‑85 (MQ=255)                                                                                                                                                  aaTTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt  <  7:188883/85‑1 (MQ=255)                                                                                                                                                    |                                                                                   CAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGT  >  NC_000913/2947318‑2947547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑