Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	757,972:1	+A	7.2%	coding (284/717 nt)	sdhB →	succinate dehydrogenase, FeS subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	757,972	1	.	A	7.2%	313.7 / 22.3	83	coding (284/717 nt)	sdhB	succinate dehydrogenase, FeS subunit
Reads supporting (aligned to +/- strand):  ref base . (39/38);  new base A (3/3);  total (42/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

TCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGA  >  NC_000913/757826‑758119                                                                                                                                                    |                                                                                                                                                     tCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑a                                                                                                                                                    <  8:104214/148‑1 (MQ=255)       ccTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTa                                                                                                                                              <  1:222437/148‑1 (MQ=255)           tCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTAcc                                                                                                                                            <  5:206672/146‑1 (MQ=255)           tCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCgg                                                                                                                                          <  1:228412/148‑1 (MQ=255)             gTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGt                                                                                                                                         <  3:321004/147‑1 (MQ=255)                gcgccgcTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAATATTCGTCTGGCCTGTATTACCCCGATTTCGGCACGCAACCTGCCGGGCAAGAAGATTGGGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGAt                                                                                                                                      <  5:82562/146‑1 (MQ=255)                ccgccgCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGAt                                                                                                                                      <  3:414726/147‑1 (MQ=255)                 cgccgcTCCTGCCGTGAAGGTGGGGGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATcc                                                                                                                                    <  6:21838/148‑1 (MQ=255)                    cgcTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑a                                                                                                                                                    >  7:84730/1‑129 (MQ=255)                    cgcTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑a                                                                                                                                                    <  8:84730/129‑1 (MQ=255)                        ccTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGAttt                                                                                                                             <  1:75557/148‑1 (MQ=255)                           gCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGAAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGAttt                                                                                                                             >  4:137401/1‑145 (MQ=255)                            ccGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTtg                                                                                                                            <  3:137401/145‑1 (MQ=255)                                 aaGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAg                                                                                                                     <  2:54819/147‑1 (MQ=255)                                  aGGTGTGGGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGAc                                                                                                                   <  6:53767/148‑1 (MQ=255)                                    gtgtgtGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACAt                                                                                                                 <  1:281355/148‑1 (MQ=255)                                     tgtgtgCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATg                                                                                                                <  5:425196/148‑1 (MQ=255)                                     tgtgtgCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATg                                                                                                                <  8:254204/148‑1 (MQ=255)                                          gCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGAc                                                                                                            >  1:351753/1‑147 (MQ=255)                                           cGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACAtaacagc                                                                                                           >  1:166059/1‑141 (MQ=255)                                           cGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACa                                                                                                           <  7:446656/147‑1 (MQ=255)                                            ggTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAAt                                                                                                         <  1:160702/148‑1 (MQ=255)                                                ccGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCt                                                                                                      <  7:22159/147‑1 (MQ=255)                                                ccGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATACGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCt                                                                                                      <  6:140321/147‑1 (MQ=255)                                                ccGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTGCGGCACGCTACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGCTCCGCGATTTGGTGGTAGACATGGGACAATTCTa                                                                                                     <  5:96307/148‑1 (MQ=255)                                                 cGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCt                                                                                                      <  8:87765/148‑1 (MQ=255)                                                        cTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAat                                                                                             <  4:234334/148‑1 (MQ=255)                                                         tGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCt                                                                                                      <  3:21701/140‑1 (MQ=255)                                                         tGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCt                                                                                                      >  4:21701/1‑140 (MQ=255)                                                          gAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACATTTCTATGCGAAatat                                                                                           >  6:52190/1‑148 (MQ=255)                                                           aaCATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATg                                                                                          <  2:113713/148‑1 (MQ=255)                                                            aCATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAatat                                                                                           >  6:142723/1‑146 (MQ=255)                                                                gAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGaa                                                                                      >  6:304388/1‑147 (MQ=255)                                                                   cGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAAt                                                                                   >  3:220879/1‑147 (MQ=255)                                                                    ggCAAGAATGGGCTGGCCTGTATTACCCCGATTTCGGCACTCATCCAGGCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTTGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTa                                                                                 <  6:224037/148‑1 (MQ=255)                                                                      cAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAg                                                                               <  2:351753/148‑1 (MQ=255)                                                                       aataaTGTTCTGGCCTGTATTACCCCGATTTCTGCACTCAACCAGCCGTTCAAGAAGATTGTGATTCGCCCGCTGCA‑‑AGGTTTACAAGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGAGCAATATGAGAAAATTAAg                                                                               <  5:185674/144‑1 (MQ=255)                                                                       aaGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTa                                                                                 >  8:332242/1‑145 (MQ=255)                                                                       aaGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTa                                                                                 <  7:332242/145‑1 (MQ=255)                                                                         gAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCt                                                                            >  5:227593/1‑148 (MQ=255)                                                                           aTGTTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCTGTGATCCTCGATTTGGTGGTAGACATGGGACAATTCTATTATCAATATGAGAAAATTAAGCAtt                                                                           >  4:102932/1‑147 (MQ=255)                                                                                cTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGt                                                                     >  8:267541/1‑148 (MQ=255)                                                                                   gCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGtt                                                                    <  5:142723/146‑1 (MQ=255)                                                                                   gCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTg                                                                   >  8:276531/1‑147 (MQ=255)                                                                                       gTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGaataa                                                              >  7:53859/1‑148 (MQ=255)                                                                                        tATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGaata                                                               <  5:304388/146‑1 (MQ=255)                                                                                           tACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTAcc                                                                        <  8:249343/134‑1 (MQ=255)                                                                                           tACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTAcc                                                                        >  7:249343/1‑134 (MQ=255)                                                                                           tACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGa                                                          >  6:187951/1‑148 (MQ=255)                                                                                           tACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGAGTTGGTGGTAGACATGGGACACTTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGa                                                          >  3:224554/1‑148 (MQ=255)                                                                                             ccccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGAc                                                         >  6:457622/1‑147 (MQ=255)                                                                                              cccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATgg                                                           >  1:133162/1‑144 (MQ=255)                                                                                              cccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATgg                                                           <  2:133162/144‑1 (MQ=255)                                                                                              cccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACa                                                        >  5:28774/1‑147 (MQ=255)                                                                                               ccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACaa                                                       >  7:447885/1‑147 (MQ=255)                                                                                               ccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACaa                                                       >  4:40296/1‑147 (MQ=255)                                                                                                cGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACa                                                        >  3:437890/1‑147 (MQ=255)                                                                                                cGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACaaa                                                      >  8:465165/1‑147 (MQ=255)                                                                                                    ttCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATc                                                   >  5:321095/1‑148 (MQ=255)                                                                                                       ggCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCg                                                 <  6:321095/147‑1 (MQ=255)                                                                                                        gCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAg                                             >  5:333206/1‑148 (MQ=255)                                                                                                         cACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTTATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGa                                                                  <  3:131656/126‑1 (MQ=255)                                                                                                          aCTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATTGGACAATTCTATGCGCAATATGAGAAAATTAATCCTTACCTGTAGaa                                                                 >  4:131656/1‑126 (MQ=255)                                                                                                            tCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTcg                                         >  5:358023/1‑148 (MQ=255)                                                                                                            tCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTcg                                         >  8:383451/1‑148 (MQ=255)                                                                                                             cAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTcgc                                        >  8:305556/1‑148 (MQ=255)                                                                                                             cAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTcgc                                        >  1:216605/1‑148 (MQ=255)                                                                                                             cAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAAGATGAGAAAATTAAGCCGTACCTGGTGAAGAATGGACAAAATCCGCCAGCTcgc                                        >  1:223060/1‑148 (MQ=255)                                                                                                               aCCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTcgc                                        >  6:94803/1‑146 (MQ=255)                                                                                                                ccAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTtggtg                                                                                                                         >  1:133490/1‑64 (MQ=255)                                                                                                                ccAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTtggtg                                                                                                                         <  2:133490/64‑1 (MQ=255)                                                                                                                       ggCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTAc                              <  5:187951/148‑1 (MQ=255)                                                                                                                        gCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTa                               >  3:280330/1‑146 (MQ=255)                                                                                                                         cAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTagag                            <  6:227593/148‑1 (MQ=255)                                                                                                                         cAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAg                            <  3:40296/148‑1 (MQ=255)                                                                                                                         cAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAg                            <  7:465165/148‑1 (MQ=255)                                                                                                                           agaagaTTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGAATTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTGACAGAt                          >  4:273678/1‑148 (MQ=255)                                                                                                                             aagaTTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTTATCCGAGATTTGTTTGAAGACATGGGACAATTATATGCGCAATATGACATAATTAACCCTTACCTGTTGAATAATTGACAAAATCCGACAGCTCGCGAGAATTTAAAGATg                         >  3:353887/1‑147 (MQ=255)                                                                                                                               gaTTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGc                        >  6:295879/1‑146 (MQ=255)                                                                                                                                 ttGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCa                      >  7:271122/1‑146 (MQ=255)                                                                                                                                         cGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTAc                                                                         >  7:367732/1‑87 (MQ=255)                                                                                                                                         cGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAgcgc             <  4:280330/147‑1 (MQ=255)                                                                                                                                           cccGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCACAGCAGCGCGa           >  1:122263/1‑147 (MQ=255)                                                                                                                                            ccGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGaa          >  4:342194/1‑147 (MQ=255)                                                                                                                                             cGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGaaaa        <  7:383451/148‑1 (MQ=255)                                                                                                                                              gCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGGAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGaaaaa       <  6:28774/148‑1 (MQ=255)                                                                                                                                                  cc‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGaaaactcg    <  6:358023/147‑5 (MQ=255)                                                                                                                                                  cc‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACt     <  6:333206/146‑1 (MQ=255)                                                                                                                                                  cc‑‑AGGTTAACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACt     <  5:457622/146‑1 (MQ=255)                                                                                                                                                   c‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGa  >  1:48261/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                     TCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGGTCTGAACATGAACGGCAAGAATGGTCTGGCCTGTATTACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCC‑‑AGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGA  >  NC_000913/757826‑758119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑