Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 812,200 C→G 5.1% Q227E (CAA→GAA)  bioC → malonyl‑ACP O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913812,2000CG 5.1% 299.2 / 3.0 86Q227E (CAA→GAA) bioCmalonyl‑ACP O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (41/40);  new base G (2/3);  total (43/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATTATCAACATCATATTCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGAACGGATACCGAAGTGGGGAAAACTGTCGCCAGTTGTGCACTTTTACAAG  >  NC_000913/812055‑812345
                                                                                                                                                 |                                                                                                                                                 
gCATTATCAACATCATATTCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCaa                                                                                                                                                 >  7:323644/1‑148 (MQ=255)
 cATTATCAACATCATATTCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAAc                                                                                                                                                <  1:91570/148‑1 (MQ=255)
    tATCAACATCATATTCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAActgg                                                                                                                                             >  2:189919/1‑148 (MQ=255)
      tCAACATCATATTCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCGACGCATCTTCATGAAGGGCGCGACGCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAActggcc                                                                                                                                           <  5:12496/148‑1 (MQ=255)
       cAACATCATATTCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAActggcct                                                                                                                                          >  2:119244/1‑148 (MQ=255)
         aCATCATATTCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAActggcctgg                                                                                                                                        >  7:346060/1‑148 (MQ=255)
          catcatATTCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAActggcctggc                                                                                                                                       >  4:5212/1‑148 (MQ=255)
            tcatATTCAGCCCCTCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCg                                                                                                                                     <  6:210190/148‑1 (MQ=255)
            tcatATTCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCTCTGAAAGGAATCGGAGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAATTGCAGCGATTGCAACTGGCCTGGCCg                                                                                                                                     >  6:215399/1‑148 (MQ=255)
             catATTCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCa                                                                                                                                                  >  3:389702/1‑134 (MQ=255)
             catATTCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCa                                                                                                                                                  <  4:389702/134‑1 (MQ=255)
                  tCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAActgg                                                                                                                                             >  3:190958/1‑134 (MQ=255)
                  tCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAActgg                                                                                                                                             <  4:190958/134‑1 (MQ=255)
                    aGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAAcagc                                                                                                                              <  1:189919/147‑1 (MQ=255)
                    aGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTGCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAAcagc                                                                                                                              <  5:59701/147‑1 (MQ=255)
                    aGCCCATCACGCTGTGGTGTGATGCTGCGCTCAGTGCCATGCGCTCGCTGACAGGCATCGGTGCCACGCATCTTCTTGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGGTGCAGCGATTGGTACTGGCCTGGCCGCAAcagc                                                                                                                              <  6:324976/147‑1 (MQ=255)
                      cccATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAgg                                                                                                                           >  2:257477/1‑148 (MQ=255)
                      cccATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAgg                                                                                                                           >  6:386270/1‑148 (MQ=255)
                        cATCACGCTGTGGTTTGATGATGTGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAgggg                                                                                                                         <  6:352301/148‑1 (MQ=255)
                           cACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGTGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGGAAc                                                                                                                                                <  4:169554/122‑1 (MQ=255)
                           cACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGTGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGGAAc                                                                                                                                                >  3:169554/1‑122 (MQ=255)
                           cACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGGAAc                                                                                                                                                <  4:188788/122‑1 (MQ=255)
                           cACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGGAAc                                                                                                                                                >  3:188788/1‑122 (MQ=255)
                                     tttGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGa                                                                                                            >  6:383217/1‑148 (MQ=255)
                                      ttGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTgcc                                                                                                           >  1:379522/1‑146 (MQ=255)
                                           gatgCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTat                                                                                                       >  1:390619/1‑147 (MQ=255)
                                               cgcTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGGCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTatcatc                                                                                                   <  7:360183/147‑1 (MQ=255)
                                                gcTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATAAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCtt                                                                                                 <  6:233488/148‑1 (MQ=255)
                                                    aGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCttttt                                                                                              <  5:386270/147‑1 (MQ=255)
                                                      tGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTg                                                                                           <  5:262909/148‑1 (MQ=255)
                                                      tGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTg                                                                                           <  1:119244/148‑1 (MQ=255)
                                                      tGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTg                                                                                           <  1:80275/148‑1 (MQ=255)
                                                      tGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTg                                                                                           >  2:317730/1‑148 (MQ=255)
                                                        ccATGCGTTCGCTGAAAGGCATCGGTGGCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTggg                                                                                         <  2:428154/148‑1 (MQ=255)
                                                        ccATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCTATATCCTCTGACGTATCATCTTTTTTTggg                                                                                         <  1:257477/148‑1 (MQ=255)
                                                        ccATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTggg                                                                                         <  7:328838/148‑1 (MQ=255)
                                                          aTGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAg                                                                                       <  3:5212/148‑1 (MQ=255)
                                                           tGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGt                                                                                      >  5:373367/1‑148 (MQ=255)
                                                           tGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGt                                                                                      >  5:202811/1‑148 (MQ=255)
                                                            gCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGt                                                                                      >  8:128581/1‑147 (MQ=255)
                                                            gCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGt                                                                                      >  8:472107/1‑147 (MQ=255)
                                                            gCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTg                                                                                     >  2:334607/1‑148 (MQ=255)
                                                                     gAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGCCCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCACCTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGCCGTATCATCTTTTTTTGGGAGTGATTGCTCGt                                                                            >  6:296875/1‑148 (MQ=255)
                                                                     gAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGt                                                                            >  7:283171/1‑148 (MQ=255)
                                                                      aaaTGCATCGGTGCCACGCATCTTCATTAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTTCAGCTATTGCAACTTGAGTGGCCGCAACAGCAGTGGCGATATCATCTGACGTATCATCTTTTTTTGTTAGTGATTGCTCGTg                                                                           <  4:157483/148‑1 (MQ=255)
                                                                      aaaGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTTCAGCGATTGCAACTGGTGTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTg                                                                           <  3:371490/148‑1 (MQ=255)
                                                                          gCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTa                                                                       <  5:89562/148‑1 (MQ=255)
                                                                           cATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTaa                                                                      <  8:146290/148‑1 (MQ=255)
                                                                              cGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTg                                                                                           <  7:286844/124‑1 (MQ=255)
                                                                              cGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTg                                                                                           >  8:286844/1‑124 (MQ=255)
                                                                              cGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACg                                                                   >  3:280450/1‑148 (MQ=255)
                                                                              cGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACg                                                                   >  3:286306/1‑148 (MQ=255)
                                                                                   ccACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTAtt                                                              <  7:128581/148‑1 (MQ=255)
                                                                                    cACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAAAAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTAttt                                                             <  7:472107/148‑1 (MQ=255)
                                                                                      cGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTg                                                           >  3:378798/1‑148 (MQ=255)
                                                                                      cGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTg                                                           >  2:364746/1‑148 (MQ=255)
                                                                                          tCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCAc                                                       >  5:349590/1‑148 (MQ=255)
                                                                                           cTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCttttt                                                                                              <  7:8040/108‑1 (MQ=255)
                                                                                           cTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCttttt                                                                                              >  8:8040/1‑108 (MQ=255)
                                                                                           cTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGa                                                                                        <  5:96933/114‑1 (MQ=255)
                                                                                           cTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGa                                                                                        >  6:96933/1‑114 (MQ=255)
                                                                                           cTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGt                                                          >  2:429638/1‑144 (MQ=255)
                                                                                            ttCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTTGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCg                                                     >  4:108500/1‑148 (MQ=255)
                                                                                             tCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTg                                                                                     >  2:465854/1‑115 (MQ=255)
                                                                                             tCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTg                                                                                     <  1:465854/115‑1 (MQ=255)
                                                                                              cATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCAc                                                       <  1:429638/144‑1 (MQ=255)
                                                                                               aTGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGaa                                                  <  5:383217/148‑1 (MQ=255)
                                                                                                tGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTAtttt                                                            <  8:283405/136‑1 (MQ=255)
                                                                                                tGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTAtttt                                                            >  7:283405/1‑136 (MQ=255)
                                                                                                  aaGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCTCCGGAACg                                                >  5:457490/1‑147 (MQ=255)
                                                                                                         cgACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGAACGGATACCg                                         >  7:280436/1‑147 (MQ=255)
                                                                                                         cgACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGAACGGATACCGa                                        <  4:280450/148‑1 (MQ=255)
                                                                                                         cgACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGAACGGATACCGa                                        <  1:162887/148‑1 (MQ=255)
                                                                                                         cgACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGAACGGATACCGa                                        <  4:286306/148‑1 (MQ=255)
                                                                                                          gACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGa                                                   >  2:257099/1‑136 (MQ=255)
                                                                                                          gACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGa                                                   <  1:257099/136‑1 (MQ=255)
                                                                                                            cccGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGAACGGATACCGAAGt                                     <  6:202811/148‑1 (MQ=255)
                                                                                                                   atatTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGAACGGATACCGAAGTGGGGaa                               >  2:461356/1‑147 (MQ=255)
                                                                                                                               tCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGa                                                                                    <  2:386116/82‑1 (MQ=255)
                                                                                                                               tCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGa                                                                                    >  1:386116/1‑82 (MQ=255)
                                                                                                                               tCGCAGTTGCAGCGATTGCAACAGGCCTGGCGGCAACAGCAGGGGCGATATACTCTGACGTATAATCATTTTTTGGGAGAGATTGCACGTGAGTAAACGAGATTTTGTCACCGGAACGGCTACCGAAGAGGGGAATACTGTCGCCAGt                  >  2:189610/1‑148 (MQ=255)
                                                                                                                                     ttGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGAACGGATACCGAAGTGGGGAAAACTGTCGCCAGTTGTGCa            >  7:263979/1‑148 (MQ=255)
                                                                                                                                      tGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGAACGGATACCGAAGTGGGGAAAACTGTCGCCAGTTGTGc             >  8:211404/1‑146 (MQ=255)
                                                                                                                                      tGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGAACGGATACCGAAGTGGGGAAAACTGTCGCCAGTTGTGc             <  7:211404/146‑1 (MQ=255)
                                                                                                                                             aTTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGGATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGAACGGATACCGAAGTGGGGAAAACTGTCGCCAGTTGTGCACTTTTACa    <  1:364746/148‑1 (MQ=255)
                                                                                                                                                gCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACAGGAACGGATACCGAAGTGGGGAAAACTGTCGCCAGTTGTGCACTTTTACAAg  <  6:373367/147‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCATTATCAACATCATATTCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCGCTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGTTCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACGTATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAAACGTTATTTTGTCACCGGAACGGATACCGAAGTGGGGAAAACTGTCGCCAGTTGTGCACTTTTACAAG  >  NC_000913/812055‑812345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: