Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,240,641 C→T 6.0% P477P (CCG→CCA cvrA ← putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,240,6410CT 6.0% 316.3 / 5.9 99P477P (CCG→CCAcvrAputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (44/49);  new base T (3/3);  total (47/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

GCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGT  >  NC_000913/1240496‑1240779
                                                                                                                                                 |                                                                                                                                          
gctTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCg                                                                                                                                           >  7:280797/1‑147 (MQ=255)
     tCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGa                                                                                                                                      <  5:316128/147‑1 (MQ=255)
     tCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGAt                                                                                                                                     <  8:280797/148‑1 (MQ=255)
      cACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGa                                                                                                                                      <  5:138763/146‑1 (MQ=255)
      cACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGa                                                                                                                                      >  6:138763/1‑146 (MQ=255)
       aCGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGAtag                                                                                                                                   >  1:377234/1‑146 (MQ=255)
       aCGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATcg                                                                                                                                   <  3:157925/148‑1 (MQ=255)
         gATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATcgcg                                                                                                                                 >  1:122532/1‑148 (MQ=255)
             ttCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGtt                                                                                                                               >  1:282032/1‑146 (MQ=255)
             ttCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGtt                                                                                                                               <  2:282032/146‑1 (MQ=255)
               ctcGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGTCTTCGAGAATTAAGTCACCAAAGAATCGATGATCCATCGCGACCGGCTGCGACTGGCTGTACAGTTTACCGAGCGCCGGAAGATCGCGTTccc                                                                                                                            >  3:242455/1‑147 (MQ=255)
                 cGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGCTCGCGTTCCCGAc                                                                                                                         >  5:80074/1‑148 (MQ=255)
                 cGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGAc                                                                                                                         >  6:304012/1‑148 (MQ=255)
                   cccGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCa                                                                                                                       <  5:304012/148‑1 (MQ=255)
                           ccAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTac                                                                                                                  <  7:481249/145‑1 (MQ=255)
                             aGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTAcacaca                                                                                                             >  5:406975/1‑148 (MQ=255)
                              ggCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGCATGAAGTCACCAAAGCAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGAACGCGTTCCCGCCCAAGTAcacacac                                                                                                            >  6:170184/1‑147 (MQ=255)
                                   tatatCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCTAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACAt                                                                                                       >  1:285710/1‑148 (MQ=255)
                                   tatatCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACAt                                                                                                       >  1:179061/1‑148 (MQ=255)
                                    atatCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCTGGAAGATCGCGTTCCCGACCAATTACACACAACACATc                                                                                                      >  6:281769/1‑148 (MQ=255)
                                    atatCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATc                                                                                                      >  5:260033/1‑148 (MQ=255)
                                    atatCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATc                                                                                                      >  1:316086/1‑148 (MQ=255)
                                     tatCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATc                                                                                                      <  3:69043/147‑1 (MQ=255)
                                        cAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCt                                                                                                  >  7:130503/1‑148 (MQ=255)
                                        cAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCt                                                                                                  >  3:52306/1‑148 (MQ=255)
                                           cgcAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTcg                                                                                               <  6:115441/148‑1 (MQ=255)
                                              aaCATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCgcgc                                                                                            <  7:296575/148‑1 (MQ=255)
                                              aaCATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCgcgc                                                                                            <  7:296579/148‑1 (MQ=255)
                                              aaCATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCgcgc                                                                                            <  3:230711/148‑1 (MQ=255)
                                              aaCATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCgcgc                                                                                            <  1:24497/148‑1 (MQ=255)
                                                cATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAATGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGCGCGCCGGAAGATCGCGTTCCCGCCCAATTACACACAACACATCGCCTTCGCGCAg                                                                                          >  4:4372/1‑148 (MQ=255)
                                                   cAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTAATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGATCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCg                                                                                       >  1:359121/1‑148 (MQ=255)
                                                    aGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCg                                                                                       <  5:48922/147‑1 (MQ=255)
                                                    aGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCg                                                                                       <  8:4024/147‑1 (MQ=255)
                                                    aGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCg                                                                                       <  2:96274/147‑1 (MQ=255)
                                                         atTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGtgct                                                                                 >  3:390000/1‑148 (MQ=255)
                                                         atTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGtgct                                                                                 >  4:337548/1‑148 (MQ=255)
                                                         atTTGGCGCTGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCgcgc                                                                                            >  8:139077/1‑136 (MQ=255)
                                                         atTTGGCGCTGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCgcgc                                                                                            <  7:139077/136‑1 (MQ=255)
                                                            tGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATACAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGCCCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGcc                                                                              >  7:342089/1‑148 (MQ=255)
                                                               cgcTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGt                                                                           <  3:220508/148‑1 (MQ=255)
                                                                gcTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTa                                                                          <  2:226100/148‑1 (MQ=255)
                                                                 cTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAg                                                                         >  2:201396/1‑148 (MQ=255)
                                                                  tGGCTTCGTGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGGGCTGCCGGTAg                                                                         >  8:468154/1‑147 (MQ=255)
                                                                    gCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCTACTGGATGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAgg                                                                        <  1:372336/146‑1 (MQ=255)
                                                                    gCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAgg                                                                        <  6:260033/146‑1 (MQ=255)
                                                                     cTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGAt                                                                      >  4:64543/1‑147 (MQ=255)
                                                                     cTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGAt                                                                      >  4:133449/1‑147 (MQ=255)
                                                                          agaATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCa                                                                <  4:52306/148‑1 (MQ=255)
                                                                           gaATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCGGCCGGTAGGATGAAGCaa                                                               <  1:378342/148‑1 (MQ=255)
                                                                             ctGAAGTCTCCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGTCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCGGCGGGTAGGATGAAGAAACt                                                             <  4:242455/147‑1 (MQ=255)
                                                                               gAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCa                                                                <  3:421971/143‑1 (MQ=255)
                                                                               gAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCa                                                                >  4:421971/1‑143 (MQ=255)
                                                                               gAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTgg                                                           >  6:38029/1‑148 (MQ=255)
                                                                                 aGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGtt                                                         <  1:165937/148‑1 (MQ=255)
                                                                                 aGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCCCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGtt                                                         <  2:316086/148‑1 (MQ=255)
                                                                                  gacACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCAGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGGAGGATGAAGCAACTGGtt                                                         >  1:233907/3‑147 (MQ=255)
                                                                                   tCACCAAAGTAGCGTTGTTCCAGCGCGACGGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTAt                                                       <  4:218816/148‑1 (MQ=255)
                                                                                   tCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTAt                                                       >  2:408443/1‑148 (MQ=255)
                                                                                    cACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAgg                                                                        <  5:53694/130‑1 (MQ=255)
                                                                                    cACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAgg                                                                        >  6:53694/1‑130 (MQ=255)
                                                                                     aCCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCa                                                     <  8:130503/148‑1 (MQ=255)
                                                                                       cAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCTGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCggg                                                                                     <  8:31717/114‑1 (MQ=255)
                                                                                       cAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCTGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCggg                                                                                     >  5:264055/1‑114 (MQ=255)
                                                                                       cAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCTGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCggg                                                                                     >  7:31717/1‑114 (MQ=255)
                                                                                       cAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCTGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCggg                                                                                     <  6:264055/114‑1 (MQ=255)
                                                                                       cAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGtt                                                                                                                               <  5:273501/72‑1 (MQ=255)
                                                                                       cAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGtt                                                                                                                               >  6:273501/1‑72 (MQ=255)
                                                                                       cAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCGGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCAc                                                    >  3:102983/1‑147 (MQ=255)
                                                                                       cAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCAc                                                    >  6:124326/1‑147 (MQ=255)
                                                                                         aagaagCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGa                                                  >  6:271132/1‑147 (MQ=255)
                                                                                          agaagCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGaaa                                                <  3:64543/148‑1 (MQ=255)
                                                                                          agaagCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGaaa                                                >  6:319787/1‑148 (MQ=255)
                                                                                                  tGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAgg                                                                        <  3:321382/116‑1 (MQ=255)
                                                                                                  tGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAgg                                                                        >  4:321382/1‑116 (MQ=255)
                                                                                                   gATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGAACAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCg                                        <  2:377234/147‑1 (MQ=255)
                                                                                                    aTCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGc                                       <  2:179061/147‑1 (MQ=255)
                                                                                                       cAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAAt                                    <  1:201396/147‑1 (MQ=255)
                                                                                                       cAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAAt                                    <  8:342089/147‑1 (MQ=255)
                                                                                                       cAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAAt                                    <  2:458543/147‑1 (MQ=255)
                                                                                                         gcgcGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCTGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATAcg                                 <  5:281769/148‑1 (MQ=255)
                                                                                                           gcgACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATAcgcg                               <  5:124326/148‑1 (MQ=255)
                                                                                                                        aCTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCat                  <  4:236947/148‑1 (MQ=255)
                                                                                                                           ggCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTAGCGCAGTCGGGTGCTGCCGGTAGGATGa                                                                    <  5:265778/95‑1 (MQ=255)
                                                                                                                           ggCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTAGCGCAGTCGGGTGCTGCCGGTAGGATGa                                                                    >  6:265778/1‑95 (MQ=255)
                                                                                                                            gCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCatat                <  5:271132/146‑1 (MQ=255)
                                                                                                                               gAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTg                                           <  6:62026/116‑1 (MQ=255)
                                                                                                                               gAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGGAGGATGAAGCAACTGGTTATCACGAAACCGTg                                           >  5:62026/1‑116 (MQ=255)
                                                                                                                                aaCAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGTTTATCACGAAACAGTGCCGCAATCCGCGTCTCTTTTGGCATATGCAAAt          >  6:71886/1‑148 (MQ=255)
                                                                                                                                aaCAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCaa            <  1:408443/146‑1 (MQ=255)
                                                                                                                                     tttACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATcgcg      >  3:260812/1‑147 (MQ=255)
                                                                                                                                     tttACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCgcgc     <  5:71886/148‑1 (MQ=255)
                                                                                                                                     tttACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCgcgc     <  3:337548/148‑1 (MQ=255)
                                                                                                                                      ttACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGa                                                                    >  7:201520/1‑84 (MQ=255)
                                                                                                                                      ttACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGa                                                                    <  8:201520/84‑1 (MQ=255)
                                                                                                                                      ttACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATcgcg      >  3:411942/1‑146 (MQ=255)
                                                                                                                                         ccGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGt  <  2:122532/147‑1 (MQ=255)
                                                                                                                                           gAGCTCCGGAAGATCGCGTTCCCGACCATTTACACACAACACATCGCCTTCGCGCAGTCGGGTGGTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCatat                <  8:57364/131‑1 (MQ=255)
                                                                                                                                           gAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCatat                >  7:57364/1‑131 (MQ=255)
                                                                                                                                                 |                                                                                                                                          
GCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGT  >  NC_000913/1240496‑1240779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: