Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,357,848 A→G 6.1% pseudogene (45/198 nt) ypaA → pseudogene, C‑terminal fragment, Transposase_31 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,357,8480AG 6.1% 296.9 / 6.1 99pseudogene (45/198 nt)ypaApseudogene, C‑terminal fragment, Transposase_31 family
Reads supporting (aligned to +/- strand):  ref base A (53/37);  new base G (4/2);  total (58/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

TAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGAATAA  >  NC_000913/2357707‑2357994
                                                                                                                                             |                                                                                                                                                  
tAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACATCCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCa                                                                                                                                               >  8:89883/1‑147 (MQ=255)
tAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCAGTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCa                                                                                                                                               >  6:419777/1‑147 (MQ=255)
  aaaTTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCaa                                                                                                                                              <  8:318895/146‑1 (MQ=255)
  aaaTTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCaa                                                                                                                                              >  7:318895/1‑146 (MQ=255)
      tGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATtggc                                                                                                                                        >  8:16370/1‑148 (MQ=255)
                 tGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGAAAACCCAACTCACTGATGATGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAgg                                                                                                                             >  8:359330/1‑148 (MQ=255)
                     ggAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaaa                                                                                                                         >  2:463491/1‑148 (MQ=255)
                     ggAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTAACTGATGCTGATATTCCGCACTGTCATTAAAAGGAGAGATTAATGAGTATTGCTGAACGGCTGCGGCAGGAAGTACATCAAATTGGCTGGGAGGGAGGTaag                                                                                                                         >  4:267228/1‑147 (MQ=255)
                       aGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATtgg                                                                                                                                         <  2:82510/130‑1 (MQ=255)
                       aGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATtgg                                                                                                                                         >  1:82510/1‑130 (MQ=255)
                          aGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGa                                                                                                                    <  1:384961/148‑1 (MQ=255)
                             gCTTTGAACGTGACATTGTGCTGGCGACAACCCAACTCACTGATGCAGATATTGCAAACTGTCAGTAAAAGTAAACATTATTGAATTTTGGTGGAGGACGCAGGCAGTTAGAACATAAAATTGGATGGCCGTAAGTTAAAtatg                                                                                                                     >  2:427990/1‑141 (MQ=255)
                                 tGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGtt                                                                                                               <  5:419777/146‑1 (MQ=255)
                                  gAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTg                                                                                                             >  7:94605/1‑147 (MQ=255)
                                   aaCGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGc                                                                                                            <  7:281424/147‑1 (MQ=255)
                                     cGTGAGATTGTGCTGGCGACAACCCAACTCACTGAGGCTGATATTCCGCACTGTCATTATAAGGTGAGATTAATGACTATTGCTGAACGGCTGCGGGAGGAAGGCCATCAAATTGGCTGGCAGGAAGGTAAAGTAGAAGGTTTGCag                                                                                                          >  1:121941/1‑146 (MQ=25)
                                      gTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATg                                                                                                         >  4:99020/1‑147 (MQ=255)
                                       tGAGATTGTGCTGGCGACCACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGaa                                                                                                       >  4:225444/1‑148 (MQ=255)
                                       tGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGaa                                                                                                       >  6:55619/1‑148 (MQ=255)
                                       tGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGaa                                                                                                       <  5:55619/148‑1 (MQ=255)
                                       tGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGaa                                                                                                       >  1:402501/1‑148 (MQ=255)
                                           aTTGTGCTGGCGACCACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCATATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAg                                                                                                   >  1:105982/1‑148 (MQ=255)
                                           aTTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaaa                                                                                                                         >  4:79023/1‑126 (MQ=255)
                                           aTTGTGCTGGCGACAACCCAACTCACTGATGCGGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaaa                                                                                                                         <  3:79023/126‑1 (MQ=255)
                                                    gCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTaaaa                                                                                          <  7:16370/148‑1 (MQ=255)
                                                     cGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAAt                                                                                         >  2:231502/1‑148 (MQ=255)
                                                       aCAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGTTTAATGACTATTGCTGAACGGCTGCTGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGc                                                                                                            <  8:467030/127‑1 (MQ=255)
                                                       aCAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGc                                                                                                            >  7:467030/1‑127 (MQ=255)
                                                        cAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGc                                                                                      >  1:287997/1‑148 (MQ=255)
                                                        cAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGc                                                                                      >  1:294752/1‑148 (MQ=255)
                                                         atcccAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCt                                                                                     <  7:89883/146‑1 (MQ=255)
                                                          aCCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCtt                                                                                    <  1:463491/148‑1 (MQ=255)
                                                           cccAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGa                                                                                                        >  2:257263/1‑127 (MQ=255)
                                                           cccAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGa                                                                                                        <  1:257263/127‑1 (MQ=255)
                                                           cccAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCttt                                                                                   >  1:249322/1‑148 (MQ=255)
                                                             cAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTgc                                                                                 >  3:237561/1‑148 (MQ=255)
                                                               aCTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTCATGACTATTGCTGAAAGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTgcgc                                                                               <  2:287997/148‑1 (MQ=255)
                                                               aCTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTgcg                                                                                >  1:68241/1‑147 (MQ=255)
                                                               aCTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTgcg                                                                                >  8:247622/1‑147 (MQ=255)
                                                                cTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTACTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCa                                                                              >  6:267282/1‑148 (MQ=255)
                                                                  cACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAAATGCTTTGCGCATg                                                                            >  4:183036/1‑148 (MQ=255)
                                                                   aCTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCAt                                                                             <  3:225444/146‑1 (MQ=255)
                                                                   aCTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCAt                                                                             <  2:402501/146‑1 (MQ=255)
                                                                    cTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCt                                                                          >  7:436479/1‑148 (MQ=255)
                                                                       aTGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGa                                                                       <  3:159012/148‑1 (MQ=255)
                                                                         gCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaa                                                                                                                          <  3:155183/95‑1 (MQ=255)
                                                                         gCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaa                                                                                                                          >  4:155183/1‑95 (MQ=255)
                                                                            gATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAgg                                                                  >  4:118120/1‑148 (MQ=255)
                                                                                 tCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTaaaa                                                                                          >  1:347815/1‑119 (MQ=255)
                                                                                 tCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTaaaa                                                                                          <  2:347815/119‑1 (MQ=255)
                                                                                    gAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAGCAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGAt                                                          >  3:179716/1‑148 (MQ=255)
                                                                                    gAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGAt                                                          <  7:359330/148‑1 (MQ=255)
                                                                                    gAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGAt                                                          <  3:118120/148‑1 (MQ=255)
                                                                                           cATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGaa                                                                                                                               >  7:468323/1‑72 (MQ=255)
                                                                                           cATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGaa                                                                                                                               <  8:468323/72‑1 (MQ=255)
                                                                                           cATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGAc                                                    >  3:133747/1‑147 (MQ=255)
                                                                                            aTTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCa                                                  <  1:231502/148‑1 (MQ=255)
                                                                                             ttAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCa                                                  <  7:106666/147‑1 (MQ=255)
                                                                                             ttAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCa                                                  >  8:106666/1‑147 (MQ=255)
                                                                                             ttAAAAGAAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAg                                                 >  4:127726/1‑148 (MQ=255)
                                                                                              tAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAgg                                                >  5:427590/1‑148 (MQ=255)
                                                                                              tAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAgg                                                >  3:145206/1‑148 (MQ=255)
                                                                                                aaaGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaa                                                                                                                          <  1:23278/72‑1 (MQ=40)
                                                                                                aaaGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTg                                              >  2:62328/1‑148 (MQ=255)
                                                                                                aaaGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaa                                                                                                                          >  2:23278/1‑72 (MQ=14)
                                                                                                     agagaTTAATGACTATTGCTGAACGGCTTCGGCAGGAAGGTCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGt                                                                                                                <  4:184446/77‑1 (MQ=255)
                                                                                                     agagaTTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACAt                                                                                                                                                 >  7:419250/1‑44 (MQ=255)
                                                                                                     agagaTTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACAt                                                                                                                                                 <  8:419250/44‑1 (MQ=255)
                                                                                                     agagaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGt                                                                                                                >  3:184446/1‑77 (MQ=255)
                                                                                                          ttAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAAc                                                                     <  6:218113/115‑1 (MQ=255)
                                                                                                          ttAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAAc                                                                     >  5:218113/1‑115 (MQ=255)
                                                                                                            aaTGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAATTTGCGTTGCGCATGCTGGATCAGGGCTTTGATCGTTACCCGGTGCTCGCGGCCAcc                                  >  2:363703/1‑148 (MQ=37)
                                                                                                              tGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCAcc                                  >  7:45448/1‑146 (MQ=255)
                                                                                                               gACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAg                               >  7:319169/1‑148 (MQ=255)
                                                                                                                   agtGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGATTGCATGAACAAGCCATTAAACTTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTaa                           <  3:267228/146‑1 (MQ=255)
                                                                                                                   aTTGCTGCACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTaa                           >  6:10192/1‑148 (MQ=255)
                                                                                                                   aTTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCt                             >  1:34942/1‑146 (MQ=255)
                                                                                                                   aTTGCTGAACGGCTCCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGtt                                                                                                               <  5:179064/64‑1 (MQ=255)
                                                                                                                   aTTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGtt                                                                                                               >  6:179064/1‑64 (MQ=255)
                                                                                                                   aTTGCTGAACGGCTACGGCAGGAAGGCCATCAAATTGGCTGGCAGGAAGGTAAAtt                                                                                                                       <  1:253329/56‑1 (MQ=11)
                                                                                                                   aTTGCTGAACGGCTACGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAAtt                                                                                                                       <  1:246280/56‑1 (MQ=255)
                                                                                                                     tGCTGAACGGCTGCGGTAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGc                         >  6:266738/1‑148 (MQ=255)
                                                                                                                         gAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAAc                                                                     <  2:88359/100‑1 (MQ=255)
                                                                                                                         gAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAAc                                                                     >  1:88359/1‑100 (MQ=255)
                                                                                                                         gAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAg                     >  4:112992/1‑148 (MQ=255)
                                                                                                                          aaCGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAg                     <  2:68241/147‑1 (MQ=255)
                                                                                                                          aaCGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAg                     <  5:267282/147‑1 (MQ=255)
                                                                                                                           aCGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAgg                                                                  >  2:215679/1‑101 (MQ=255)
                                                                                                                           aCGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAgg                                                                  <  1:215679/101‑1 (MQ=255)
                                                                                                                                 acGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAg                                                                                                                     >  6:331822/2‑44 (MQ=11)
                                                                                                                                 acGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAg                                                                                                                     <  5:331822/43‑1 (MQ=255)
                                                                                                                                    gCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGtt                                                                                                               >  7:346460/1‑47 (MQ=255)
                                                                                                                                    gCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGtt                                                                                                               <  8:346460/47‑1 (MQ=255)
                                                                                                                                     cAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGTTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAg         <  8:436479/148‑1 (MQ=255)
                                                                                                                                     cAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAg         <  4:237561/148‑1 (MQ=255)
                                                                                                                                      aggaaggaCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAACCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGc        >  2:217935/1‑148 (MQ=255)
                                                                                                                                         aaggtCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGaa     >  8:294456/1‑148 (MQ=255)
                                                                                                                                         aaggaCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAGCAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGaa     <  4:179716/148‑1 (MQ=255)
                                                                                                                                         aaggaCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGaa     >  7:361968/1‑148 (MQ=255)
                                                                                                                                            gaCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGAATaa  <  4:133747/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
TAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGAATAA  >  NC_000913/2357707‑2357994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: