Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,378,333 A→G 5.9% V309V (GTT→GTC menD ← 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,378,3330AG 5.9% 209.8 / 3.1 68V309V (GTT→GTCmenD2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase
Reads supporting (aligned to +/- strand):  ref base A (30/34);  new base G (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

CATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGCCTAACCAAAGATCGGCACACGG  >  NC_000913/2378189‑2378479
                                                                                                                                                |                                                                                                                                                  
cATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATcaa                                                                                                                                                   <  6:379705/146‑1 (MQ=255)
  ttGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACAAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAAt                                                                                                                                               <  2:374442/148‑1 (MQ=255)
     ccTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATcc                                                                                                                                             <  7:318866/147‑1 (MQ=255)
      cTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAg                                                                                                                                           >  3:78830/1‑148 (MQ=255)
         ttCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGt                                                                                                                                          <  2:235685/146‑1 (MQ=255)
             gCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACtctt                                                                                                                                    <  7:317870/148‑1 (MQ=255)
             gCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACtctt                                                                                                                                    <  7:353070/148‑1 (MQ=255)
              ccAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACtcttc                                                                                                                                   >  5:456648/1‑148 (MQ=255)
                 ggCGCGTGATTTCAACGCACCAGGGCTGGCGGTTTTCTGCCGGATGCAGCTCCAGCCAGGCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTgg                                                                                                                                <  6:282698/148‑1 (MQ=255)
                   cgcgGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGtt                                                                                                                              >  6:162730/1‑148 (MQ=255)
                         aTTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGGCAGTCGGCAATATTGGCAATTAAGCGACGTCGGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGc                                                                                                                        <  1:170492/148‑1 (MQ=255)
                             cAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTtg                                                                                                                     <  4:235876/147‑1 (MQ=255)
                               aCGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTtgcct                                                                                                                  >  7:415309/1‑148 (MQ=255)
                                      aGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTg                                                                                                           >  4:290374/1‑148 (MQ=255)
                                            ggCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAAt                                                                                                                                               <  8:190243/106‑1 (MQ=255)
                                            ggCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAAt                                                                                                                                               >  7:190243/1‑106 (MQ=255)
                                            ggCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGACAATATTGGCAATTATGCGACGTCCGCGATGGTGTGCTGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAg                                                                                                     >  7:141790/1‑148 (MQ=255)
                                                 ttttCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGtt                                                                                                >  3:392264/1‑148 (MQ=255)
                                                   ttCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGttt                                                                                               <  4:78830/147‑1 (MQ=255)
                                                    tCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTc                                                                                              <  2:39975/147‑1 (MQ=255)
                                                    tCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTc                                                                                              <  3:290374/147‑1 (MQ=255)
                                                      tGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTcccc                                                                                           >  4:370945/1‑148 (MQ=255)
                                                       gCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGc                                                                                                                        <  1:421330/118‑1 (MQ=255)
                                                       gCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGc                                                                                                                        >  2:421330/1‑118 (MQ=255)
                                                         cGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCACTCGCATCAACAATCCAGTACTCTTCTGGTGCACAGCTTGCCTGCCATTGCATGAGCCGTTTCCCCggc                                                                                        <  3:225184/148‑3 (MQ=255)
                                                         cGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTc                                                                                        <  6:456648/148‑1 (MQ=255)
                                                         cGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTc                                                                                        <  4:392264/148‑1 (MQ=255)
                                                                       gccagTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCGACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAg                                                                          >  3:174365/1‑148 (MQ=255)
                                                                       gccagTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCGACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAg                                                                          >  3:167926/1‑148 (MQ=255)
                                                                         cagTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATcc                                                                                                                                             <  8:390706/79‑1 (MQ=255)
                                                                         cagTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATcc                                                                                                                                             >  7:390706/1‑79 (MQ=255)
                                                                           ggaggCAATATTGGCAATCAAGCGACGTCCGCGATGGTGTGCCGGATCATGTCGCCCTTCAATGTCATCAACAATCAAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGc                                                                      <  2:434047/145‑1 (MQ=255)
                                                                           gTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGc                                                                      <  7:17060/148‑1 (MQ=255)
                                                                             cGGCAATATTGGCAATTAAGCGACGTCAGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAg                                                                                                        >  7:142755/1‑112 (MQ=255)
                                                                             cGGCAATATTGGCAATTAAGCGACGTCAGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAg                                                                                                        <  8:142755/112‑1 (MQ=255)
                                                                                     ttGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCGACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAAtt                                                             <  4:174365/147‑1 (MQ=255)
                                                                                     ttGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCGACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAAtt                                                             <  4:167926/147‑1 (MQ=255)
                                                                                      tGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTg                                                           >  3:359440/1‑148 (MQ=255)
                                                                                          aaTTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGcc                                                       >  6:50852/1‑148 (MQ=255)
                                                                                          aaTTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGcc                                                       >  5:123513/1‑148 (MQ=255)
                                                                                           aTTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCct                                                      >  6:4048/1‑148 (MQ=255)
                                                                                                  gACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGca                                                                     <  7:39630/126‑1 (MQ=255)
                                                                                                  gACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGca                                                                     >  8:39630/1‑126 (MQ=255)
                                                                                                       ccGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGc                                          >  6:385730/1‑148 (MQ=255)
                                                                                                        cgcgATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGc                                          <  8:415309/147‑1 (MQ=255)
                                                                                                         gcgATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGcacc                                                                   >  8:294239/1‑121 (MQ=255)
                                                                                                         gcgATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGcacc                                                                   <  7:294239/121‑1 (MQ=255)
                                                                                                           gATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATATGCGCCTGCTGCAGCTCGCTGGt                                      <  7:87097/148‑1 (MQ=255)
                                                                                                                 gtgCTGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCttt                                <  8:141790/148‑1 (MQ=255)
                                                                                                                  tgCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCttt                                <  5:4048/147‑1 (MQ=255)
                                                                                                                   gCCGGATCAAGTCGCCCTTCAATGTCATCAAGAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTgg                              <  3:88175/148‑1 (MQ=255)
                                                                                                                   gCCGGATCAAGTCGCCCTTCAATCTCATCAACAAACCAGTACACTTCTGGAACACCGCGTGCCTGACATTGCAGGACACGATTCCCCGTCAGCCTGCTTCCCAGTTCCCCCCCAATTAGCGCCTGCTGCAGCTCGCTGCACGCTTTgg                              >  4:345469/1‑148 (MQ=255)
                                                                                                                     cGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCa                            <  6:438408/148‑1 (MQ=255)
                                                                                                                     cGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCa                            <  4:359440/148‑1 (MQ=255)
                                                                                                                        aTCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTg                         <  6:123513/148‑1 (MQ=255)
                                                                                                                        aTCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCATGCTGCAGCTCGCTGGTCGCTTTGGCATTg                         >  5:212356/1‑148 (MQ=255)
                                                                                                                         tCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGc                        >  4:307592/1‑148 (MQ=255)
                                                                                                                             gTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGCCTaa                    >  7:104987/1‑148 (MQ=255)
                                                                                                                                 cccTTCAACGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCAGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGCCTAACCaa                <  8:477920/148‑1 (MQ=255)
                                                                                                                                     tCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCa                            >  2:279430/1‑132 (MQ=255)
                                                                                                                                     tCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCa                            <  1:279430/132‑1 (MQ=255)
                                                                                                                                     tCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGCCTAACCa                 >  7:151230/1‑143 (MQ=255)
                                                                                                                                     tCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGCCTAACCa                 >  4:62401/1‑143 (MQ=255)
                                                                                                                                     tCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGACGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGCCTAACCa                 >  1:399383/1‑143 (MQ=255)
                                                                                                                                      cAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGCCTAACCAAAGa             <  5:162730/146‑1 (MQ=255)
                                                                                                                                        aTGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGCCTAACCAAAGATCgg         >  2:67660/1‑148 (MQ=255)
                                                                                                                                          gTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCa                            >  2:216910/1‑127 (MQ=255)
                                                                                                                                          gTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCa                            <  1:216910/127‑1 (MQ=255)
                                                                                                                                          gTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGCCTAACCAAAGATCGGca       >  8:306270/1‑148 (MQ=255)
                                                                                                                                            catcaACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGCCTAACCAAAGATCGGcaca     >  8:29694/1‑148 (MQ=255)
                                                                                                                                               caacaaTCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCt                                  >  2:211950/1‑116 (MQ=255)
                                                                                                                                               caacaaTCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCt                                  <  1:211950/116‑1 (MQ=255)
                                                                                                                                               caacaaTCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGCCTAACCAAAGa             <  6:446546/137‑1 (MQ=255)
                                                                                                                                               caacaaTCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGCCTAACCAAAGa             >  5:446546/1‑137 (MQ=255)
                                                                                                                                               caacaaTCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCAAGCTGCAGCTCGATGGTCGCTTTGGCATTGCCTAACCAAAGAACGCCACACgg  >  2:104535/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATCCAGTACTCTTCTGGTTCACAGCTTGCCTGCCATTGCAGGAGCCGTTTCCCCGTCAGGCTGCTTCCCAGTTGCACCACAATTTGCGCCTGCTGCAGCTCGCTGGTCGCTTTGGCATTGCCTAACCAAAGATCGGCACACGG  >  NC_000913/2378189‑2378479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: