New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 249927628 (0.830)5 (0.160) 5/246 2.9 15.1% coding (606/1698 nt) ypdA sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system
?NC_000913 = 2499293 30 (0.940)coding (623/1698 nt) ypdA sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system
Rejected: Frequency below/above cutoff threshold.

GTAATTGTCTGGGCACCAACTACCGCGTTGGGGATCGATATCGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCAT  >  NC_000913/2499136‑2499423
                                                                                                                                            |                                                                                                                                                   
gTAATTGTCTGGGCACCAACTACCGCGTTGGGGATCGATATCGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCgg                                                                                                                                               >  2:92618/1‑147 (MQ=255)
gTAATTGTCTGGGCACCAACTACCGCGTTGGGGATCGATATCGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCa                                                                                                                                             <  5:57868/149‑1 (MQ=255)
                 aaCTACCGCGTTGGGGATCGATATCGTCTCTAAAATCGGCATTCCAATGAGCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCagcttggcctgccgcgcggcgctgg                                                                                                                            <  1:179317‑M1/149‑26 (MQ=255)
                       cgcgTTGGGGATCGATATCGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATc                                                                                                                       <  4:86839/148‑1 (MQ=255)
                       cgcgTTGGGGATCGATATCGTCTCTAAAATCGGCATTCAAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATc                                                                                                                       >  3:86839/1‑148 (MQ=255)
                              gggATCGATATCGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCcaaca                                                                                                                >  8:75003/1‑148 (MQ=255)
                                    gATATCGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCagcttggcctgccgcgcggcgctggcctctttttcgccctcaac                                                                                                         >  8:124413‑M1/1‑105 (MQ=255)
                                       atCGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCa                                                                                                                                             <  6:141830/110‑1 (MQ=255)
                                         cGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCagcttggcctgccgcgcggcgctggcctctttttcgccctcaacgcttt                                                                                                    <  7:124413‑M1/149‑50 (MQ=255)
                                              ctAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCagcttggcctgccgcgcggcgctg                                                                                                                             <  2:128928‑M1/119‑25 (MQ=255)
                                              ctAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCagcttggcctgccgcgcggcgctg                                                                                                                             >  1:128928‑M1/1‑95 (MQ=255)
                                                 aaaTCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCTGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGc                                                                                            <  6:84664/149‑1 (MQ=255)
                                                 aaaTCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATc                                                                                                                       >  6:32707/1‑122 (MQ=255)
                                                 aaaTCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATc                                                                                                                       <  5:32707/122‑1 (MQ=255)
                                                 aaaTCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGc                                                                                            >  6:67553/1‑149 (MQ=255)
                                                          ttCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCaa                                                                                                                                       <  6:54326/97‑1 (MQ=255)
                                                          ttCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCaa                                                                                                                                       >  5:54326/1‑97 (MQ=255)
                                                          ttCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCAt                                                                                         <  7:93758/143‑1 (MQ=255)
                                                          ttCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCAt                                                                                         >  8:93758/1‑143 (MQ=255)
                                                              aaTGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGt                                                                                       >  1:118130/1‑141 (MQ=255)
                                                              aaTGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGt                                                                                       <  2:118130/141‑1 (MQ=255)
                                                              aaTGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATa                                                                                  >  1:104180/1‑146 (MQ=255)
                                                              aaTGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATa                                                                                  <  2:104180/146‑1 (MQ=255)
                                                                  aTCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTc                                                                           <  5:67553/149‑1 (MQ=255)
                                                                    ccTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCAt                                                                         >  7:67126/1‑149 (MQ=255)
                                                                                              ttGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCcgc                                               <  7:75003/149‑1 (MQ=255)
                                                                                                   cTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGc                                                           <  4:110804/132‑1 (MQ=255)
                                                                                                   cTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGc                                                           >  3:110804/1‑132 (MQ=255)
                                                                                                          tGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGaaa                                                       <  3:68993/129‑1 (MQ=255)
                                                                                                          tGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGaaa                                                       >  4:68993/1‑129 (MQ=255)
                                                                                                             aaaGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTggcgctggcctctttttcgccctcaacgctttgcaccagaagcacaataaagccgatacagacgctaccgaggatcattggaatgccgattttagagacg                                <  7:124413‑M2/149‑101 (MQ=255)
                                                                                                                  gTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTggcgctggcctctttttcgccctcaacgctttgcaccagaagcacaataaagccgatacagacgctaccgaggatcattggaatgccgattttagagacgatatc                           >  8:124413‑M2/1‑44 (MQ=255)
                                                                                                                                    gCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGGGGCGATTACTAATAc         >  3:19307/1‑149 (MQ=255)
                                                                                                                                     ccAGCGCCGCGCGGCAGGCCAAGCTggcgctggcctctttttcgccctcaacgctttgcaccagaagcacaataaagccgatacagacgctaccgaggctcattggaatgccgattttagagacgatatcgatccccaacgcggtagtt        <  1:179317‑M2/149‑125 (MQ=255)
                                                                                                                                      cAGCGCCGCGCGGCAGGCCAAGCTggcgctggcctctttttcgccctcaacgctttgcaccagaagcacaataaagccgatacagacgctaccgaggatcattggaatgccgattttag                                     <  2:128928‑M2/119‑96 (MQ=255)
                                                                                                                                      cAGCGCCGCGCGGCAGGCCAAGCTggcgctggcctctttttcgccctcaacgctttgcaccagaagcacaataaagccgatacagacgctaccgaggatcattggaatgccgattttag                                     >  1:128928‑M2/1‑24 (MQ=255)
                                                                                                                                      cAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATAcc        >  1:61212/1‑148 (MQ=255)
                                                                                                                                           ccgcGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACAATCACGCCGATGCGGTGGCGATTACTAATACCGATCAt  <  8:67126/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
GTAATTGTCTGGGCACCAACTACCGCGTTGGGGATCGATATCGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCAT  >  NC_000913/2499136‑2499423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.