New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3705896 =56 (1.650)6 (0.190) 6/246 2.6 10.2% intergenic (‑106/+202) dppB/dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
?NC_000913 3705924 = NA (NA)intergenic (‑134/+174) dppB/dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Rejected: Frequency below/above cutoff threshold.

GGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAA  >  NC_000913/3705750‑3706044
                                                                                                                                                  |                                                                                                                                                    
gggATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATg                                                                                                                                                     <  3:121044/148‑1 (MQ=255)
     gACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTg                                                                                                                                               >  5:156139/1‑149 (MQ=255)
     aaatgcctgatgcgctccgcttatcaggcctacgaaaattctgcaatgtattgaatttgcacgattttgtaggccggataaggcgttaacgccgcatccggcataaacaaagcgcactttgtcaacaatctgtatacccggTGGCGCTg                                                                                                                                               <  7:27009‑M1/8‑1 (MQ=255)
           tCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACg                                                                                                                                           >  1:137141/1‑147 (MQ=255)
           tCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACg                                                                                                                                           >  2:177946/1‑147 (MQ=255)
                       gagaATAAACTGCAACATAACCCGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAgcgc                                                                                                                             >  5:147142/1‑149 (MQ=255)
                               aCTGCAACATAACCCGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCggg                                                                                                                      >  8:25183/1‑148 (MQ=255)
                                  gCAACATAACCCGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGtata                                                                                                                  >  5:137009/1‑149 (MQ=255)
                                     aCATAACCCGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGtata                                                                                                                  >  6:148080/1‑146 (MQ=255)
                                           cccGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGAttgtt                                                                                                         <  6:147142/149‑1 (MQ=255)
                                           cccGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGAttgtt                                                                                                         <  7:25183/149‑1 (MQ=255)
                                            ccGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGcac                                                                                                                                  >  4:105393/1‑123 (MQ=255)
                                            ccGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGcac                                                                                                                                  <  3:105393/123‑1 (MQ=255)
                                            ccGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGAttgtt                                                                                                         >  6:112547/1‑148 (MQ=255)
                                                 aatgtattgaatttgcacgattttgtaggccggataaggcgttaacgccgcatccggcataaacaaagcgcactttgtcaacaatctgtatacccggTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACa                                                                                                     <  8:57754‑M1/50‑1 (MQ=255)
                                                             aCCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGt                                                                                        <  6:156139/148‑1 (MQ=255)
                                                                cGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTAt                                                                                    <  6:137009/149‑1 (MQ=255)
                                                                 gCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGAGTGTTGACAAAGTGCGCTTTGTTTATg                                                                                   >  8:27009/1‑149 (MQ=255)
                                                                   gattttgtaggccggataaggcgttaacgccgcatccggcataaacaaagcgcactttgtcaacaatctgtatacccggTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGAttgtt                                                                                                         >  6:3814‑M1/80‑125 (MQ=255)
                                                                   gattttgtaggccggataaggcgttaacgccgcatccggcataaacaaagcgcactttgtcaacaatctgtatacccggTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGAttgtt                                                                                                         <  5:3814‑M1/46‑1 (MQ=255)
                                                                       tGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGAttgt                                                                                                          <  4:118136/120‑1 (MQ=255)
                                                                          taggccggatcaggcgttaacgccgcatccggcataaacaaagcgcactttgtcaacaatctgtatacccggTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                          >  7:57754‑M1/73‑149 (MQ=255)
                                                                          aaCTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                          <  5:148080/149‑1 (MQ=255)
                                                                            ggccggataaggcgttaacgccgcatccggcataaacaaagcgcactttgtcaacaatctgtatacccggTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGAtt                                                                                                            >  7:37057‑M1/71‑113 (MQ=255)
                                                                            ggccggataaggcgttaacgccgcatccggcataaacaaagcgcactttgtcaacaatctgtatacccggTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGAtt                                                                                                            <  8:37057‑M1/43‑1 (MQ=255)
                                                                              gCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTAt                                                                                    >  8:6457/1‑135 (MQ=255)
                                                                              gCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTAt                                                                                    <  7:6457/135‑1 (MQ=255)
                                                                                   tCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGt                                                                                        <  1:169222/126‑1 (MQ=255)
                                                                                   tCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGt                                                                                        >  2:169222/1‑126 (MQ=255)
                                                                                   tCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAc                                                                  <  2:137141/148‑1 (MQ=255)
                                                                                    cTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATg                                                                                   >  4:73134/1‑130 (MQ=255)
                                                                                    cTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATg                                                                                   <  3:73134/130‑1 (MQ=255)
                                                                                     tGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACa                                                                                                                <  5:76509/100‑1 (MQ=255)
                                                                                     tGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACa                                                                                                                >  6:76509/1‑100 (MQ=255)
                                                                                     tGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                               >  1:173141/1‑149 (MQ=255)
                                                                                            cTCACAAATCAGTTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                               >  4:123822/1‑142 (MQ=255)
                                                                                            cTCACAAATCAGTTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                               <  3:123822/142‑1 (MQ=255)
                                                                                                caAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                    <  1:177946/149‑1 (MQ=255)
                                                                                                 aaaTCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                   >  2:174610/1‑149 (MQ=255)
                                                                                                           ccGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAGTCGTg                                         >  5:141236/1‑149 (MQ=255)
                                                                                                                   aTCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                   <  5:112547/147‑1 (MQ=255)
                                                                                                                          aGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCg                                                        >  6:88198/1‑119 (MQ=255)
                                                                                                                          aGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCg                                                        <  5:88198/119‑1 (MQ=255)
                                                                                                                          aGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                         >  3:42251/1‑134 (MQ=255)
                                                                                                                          aGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                         <  4:42251/134‑1 (MQ=255)
                                                                                                                          aGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                          >  8:18160/1‑149 (MQ=255)
                                                                                                                           gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                         <  6:141236/149‑1 (MQ=255)
                                                                                                                            ggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGt                                                                                                 <  3:113787/76‑1 (MQ=255)
                                                                                                                            ggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGt                                                                                                 >  4:113787/1‑76 (MQ=255)
                                                                                                                              aaTGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGt                                                                                        <  1:142320/83‑1 (MQ=255)
                                                                                                                              aaTGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGt                                                                                        >  2:142320/1‑83 (MQ=255)
                                                                                                                                tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGAttgtt                                                                                                         >  6:138179/1‑64 (MQ=255)
                                                                                                                                tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGAttgtt                                                                                                         <  5:138179/64‑1 (MQ=255)
                                                                                                                                     tATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGc                                                                                  >  7:50888/1‑82 (MQ=255)
                                                                                                                                     tATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGc                                                                                  <  8:50888/82‑1 (MQ=255)
                                                                                                                                          ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAgg          >  2:79062/1‑149 (MQ=255)
                                                                                                                                             ccGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                         <  6:96745/115‑1 (MQ=255)
                                                                                                                                             ccGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                         >  5:96745/1‑115 (MQ=255)
                                                                                                                                             ccGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTa            <  1:176432/144‑1 (MQ=255)
                                                                                                                                             ccGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTa            >  2:176432/1‑144 (MQ=255)
                                                                                                                                             ccGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCt       >  7:128403/1‑149 (MQ=255)
                                                                                                                                                gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGa     >  7:58777/1‑148 (MQ=255)
                                                                                                                                                gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGAt    <  1:174610/149‑1 (MQ=255)
                                                                                                                                                  TGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATaa  >  2:163491‑M1/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCTATAGTGACCCGCAGCCTGGAACTGCGTGTCTGTATTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAA  >  NC_000913/3705750‑3706044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.