Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 755,790 G→A W77* (TGG→TGA)  sdhD → succinate dehydrogenase, membrane subunit, binds cytochrome b556

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913755,7900GA100.0% 149.6 / NA 43W77* (TGG→TGA) sdhDsuccinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/22);  total (21/22)

TCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGATG  >  NC_000913/755660‑755934
                                                                                                                                  |                                                                                                                                                
tCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTAc                                                                                                                                >  3:96949/1‑149 (MQ=255)
             tACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACAAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTgg                                                                                                                   >  2:168691/1‑149 (MQ=255)
                cAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACACTGCTGGCGCTGTTTTCTATCTTGATCCATGACTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCtt                                                                                                                >  5:11498/1‑149 (MQ=255)
                           tGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtg                                                                                                     >  2:64573/1‑149 (MQ=255)
                              cATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgctg                                                                                                  >  3:93915/1‑149 (MQ=255)
                                tatGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTaaa                                                                                                                          <  1:20087/123‑1 (MQ=255)
                                tatGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTaaa                                                                                                                          >  2:20087/1‑123 (MQ=255)
                                  tGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAAc                                                                                              >  4:60763/1‑149 (MQ=255)
                                    aaGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTg                                                                                            >  4:29950/1‑149 (MQ=255)
                                      gTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGt                                                                                          >  3:55659/1‑149 (MQ=255)
                                                gTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGtt                                                                                >  1:102030/1‑149 (MQ=255)
                                                gTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGtt                                                                                >  4:73675/1‑149 (MQ=255)
                                                gTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGtt                                                                                <  1:141881/149‑1 (MQ=255)
                                                gTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGtt                                                                                <  1:168691/149‑1 (MQ=255)
                                                    cttcGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCa                                                                             <  4:55659/148‑1 (MQ=255)
                                                               cGTTCACCAAAGTGTTCACCCTGCTTTCGCTGTTTTCTATCTTGATCCATGCCTGGATCTGCATGTGACAGGTGTTGACCGACTACGTTAAAGCGCTTGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGTTTTAc                                                                 <  6:11498/149‑1 (MQ=255)
                                                                gTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAAc                                                                                              <  5:71199/119‑1 (MQ=255)
                                                                gTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAAc                                                                                              >  6:71199/1‑119 (MQ=255)
                                                                gTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACg                                                                >  3:27578/1‑149 (MQ=255)
                                                                gTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACg                                                                <  1:64573/149‑1 (MQ=255)
                                                                      cAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGAttt                                                          <  3:29950/149‑1 (MQ=255)
                                                                             ttCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGAtt                                                   >  2:17409/1‑149 (MQ=255)
                                                                                 cccTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGtt                                               <  2:102030/149‑1 (MQ=255)
                                                                                         gcgcTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtggtgtg                                       <  3:60763/149‑1 (MQ=255)
                                                                                           gcTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTggg                                     >  7:105084/1‑149 (MQ=255)
                                                                                               ttttCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACtggt                                                                        <  8:103875/110‑1 (MQ=255)
                                                                                                        ttGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTc                                                                                   <  4:3233/90‑1 (MQ=255)
                                                                                                        ttGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTc                                                                                   >  3:3233/1‑90 (MQ=255)
                                                                                                          gATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGgtgtgt                               <  1:134564/140‑1 (MQ=255)
                                                                                                          gATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGgtgtgt                               >  2:134564/1‑140 (MQ=255)
                                                                                                          gATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAAtt                      >  6:916/1‑149 (MQ=255)
                                                                                                           aTCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAAtt                      <  1:17409/148‑1 (MQ=255)
                                                                                                           aTCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTg                     <  5:916/149‑1 (MQ=255)
                                                                                                            tCCATGCCTGGATCGGCATGTGACAGGTGTTGACAGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGc                    <  8:105084/149‑1 (MQ=255)
                                                                                                              cATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCa                  <  4:96949/149‑1 (MQ=255)
                                                                                                               aTGCCTGGATCGGCATGTGACAGGTGTTGACCTACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtggtgtg                                       <  8:105875/127‑1 (MQ=255)
                                                                                                               aTGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtggtgtg                                       >  7:105875/1‑127 (MQ=255)
                                                                                                                  ccTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAATCCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCa              <  3:73675/149‑1 (MQ=255)
                                                                                                                     ggATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGtgatg                           <  5:30262/133‑1 (MQ=255)
                                                                                                                     ggATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGtgatg                           >  6:30262/1‑133 (MQ=255)
                                                                                                                            cATGTGACAGGTGTTGACCGACTACGTTAAACCGCTgg                                                                                                                   >  5:115310/1‑38 (MQ=255)
                                                                                                                            cATGTGACAGGTGTTGACCGACTACGTTAAACCGCTgg                                                                                                                   <  6:115310/38‑1 (MQ=255)
                                                                                                                              tgtgACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTGTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGATg  <  4:93915/149‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                                
TCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGATG  >  NC_000913/755660‑755934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑